Journal: Pharmaceuticals

Article Title: Self-Assembled Supramolecular Nanoparticles Improve the Cytotoxic Efficacy of CK2 Inhibitor THN7

doi: 10.3390/ph11010010

Figure Lengend Snippet: Cytotoxic activity of THN7-loaded α-cyclodextrin nanoparticles on A-427 lung cancer cells. C4: C 4 H 9 ; C6: C 6 H 13 ; C8: C 8 H 17 . (+) loaded cyclodextrins; (−) empty cyclodextrins. Viability of A427 cells were determined using an MTT assay. The viability of cells treated with 1% of the appropriate solvent was set 100%. Staurosporine served as a control for the correctly performance of the MTT assay using A427 cells. In all experiments THN7 was used at a final concentration of 6 μM.

Article Snippet: The nanoparticles were tested on the human lung cancer cell line A427, which was obtained from the German Collection of Microorganisms and Cell Culture (DSMZ, Braunschweig, Germany).

Techniques: Activity Assay, MTT Assay, Solvent, Control, Concentration Assay

Journal: Cells

Article Title: Dauricine Impedes the Tumorigenesis of Lung Adenocarcinoma by Regulating Nrf2 and Reactive Oxygen Species

doi: 10.3390/cells14100698

Figure Lengend Snippet: Dauricine inhibits the proliferation of NSCLC cells. Lung cancer cells ( A ) A549, ( B ) H1299, ( C ) A427, and ( D ) LLC were treated with various concentrations of dauricine (Dau) at 0, 5, 10, 15, and 20 µM for 24 h before exposed to MTT solution to assess growth progression. A colony formation assay was conducted to estimate the proliferation of lung cancer ( E , F ) A549, ( G , H ) H1299 and ( I , J ) A427 cells under the influence of dauricine at concentrations of 5 and 15 µM for 48 h. Post-treatment images were captured to perform a comparative analysis to determine the average sizes of colonies for each condition. Quantification was carried out using ImageJ, and the results are represented in columns; data are shown as mean ± SEM, with *, ** and *** indicating p < 0.05, p < 0.01 and p < 0.001, respectively. n.s., not significant.

Article Snippet: Four non-small-cell lung cancer cells were procured: A549, H1299, and Lewis Lung Carcinoma (LLC) from the American Type Culture Collection (ATCC), while A427 cells were obtained from Procell (Wuhan, China).

Techniques: Colony Assay

Journal: Cells

Article Title: Dauricine Impedes the Tumorigenesis of Lung Adenocarcinoma by Regulating Nrf2 and Reactive Oxygen Species

doi: 10.3390/cells14100698

Figure Lengend Snippet: Dauricine suppresses NSCLC cell migration. LUAD cells allowed to form a confluent monolayer were processed for uniform scratching with a 200 µL tip. ( A , B ) display H1299 cell wound recovery micrographs (Scale bar = 400 µm) of indicated concentrations and a column chart representing the quantification of wound migration. ( C , D ) A427 cell images depicting wound recovery progress along with quantification shown in column graphs for concentrations of 5 and 15 µM. Image J software was used for quantification, showing means ± SEM, with *, ** and *** indicating p < 0.05, p < 0.01 and p < 0.001 respectively.

Article Snippet: Four non-small-cell lung cancer cells were procured: A549, H1299, and Lewis Lung Carcinoma (LLC) from the American Type Culture Collection (ATCC), while A427 cells were obtained from Procell (Wuhan, China).

Techniques: Migration, Software

Journal: Cells

Article Title: Dauricine Impedes the Tumorigenesis of Lung Adenocarcinoma by Regulating Nrf2 and Reactive Oxygen Species

doi: 10.3390/cells14100698

Figure Lengend Snippet: Dauricine restrains the progression of the cell cycle and regulates cell growth. ( A ) A549, ( C ) H1299, and ( E ) A427 were exposed to doses of dauricine (5 and 15 µM) for 24 h, before EdU proliferation assay was performed as shown in the images. Post-staining images were captured using an inverted fluorescence microscope, focusing on three randomly selected fields for each condition. The quantification was analyzed for ( B ) A549, ( D ) H1299, and ( F ) A427 cells through ImageJ software and is presented in column charts. Histograms of ( G ) A549, ( I ) H1299, and ( K ) A427 illustrate the distribution of LUAD cells into distinct phases of the cell cycle after treatment with dauricine (15 µM) for 24 h. The quantification of cells in different phases is displayed on the right for ( H ) A549, ( J ) H1299, and ( L ) A427 cells. All error bars represent the standard error of the mean (n = 3), with *, ** and *** indicating p < 0.05, p < 0.01 and p < 0.001 respectively.

Article Snippet: Four non-small-cell lung cancer cells were procured: A549, H1299, and Lewis Lung Carcinoma (LLC) from the American Type Culture Collection (ATCC), while A427 cells were obtained from Procell (Wuhan, China).

Techniques: Proliferation Assay, Staining, Fluorescence, Microscopy, Software

Journal: Cells

Article Title: Dauricine Impedes the Tumorigenesis of Lung Adenocarcinoma by Regulating Nrf2 and Reactive Oxygen Species

doi: 10.3390/cells14100698

Figure Lengend Snippet: Dauricine increases the levels of ROS and reduces Nrf2 expression. ( A ) A549, ( C ) H1299, and ( E ) A427 cells were treated with dauricine at a dose of 15 µM for 24 h. Before harvesting the cells, incubation with 10 µM of DCFH-DA for 30 min was conducted to examine the ROS contents in each cell line with a flow cytometer and column charts representing the quantification of ROS in ( B ) A549, ( D ) H1299, and ( F ) A427. Cultured LUAD cells subjected to the treatment of dauricine (15 µM) with or without 10 µM of Z-VAD/fmk for 24 h were incubated with MTT solution to measure growth progression in three lung cancer cell line: ( G ) A549, ( H ) H1299, and ( I ) A427. LUAD cells were exposed to dauricine at a concentration of 15 µM for 24 h and lysates of ( J ) A549, ( L ) H1299, ( N ) A427 cells were collected to observe protein expression by Western blotting with indicated antibodies. The quantification was performed with Image Studio software and was shown in column charts for ( K ) A549, ( M ) H1299, and ( O ) A427. All error bars represent the standard error of the mean (n = 3), and * p < 0.05, ** p < 0.01.

Article Snippet: Four non-small-cell lung cancer cells were procured: A549, H1299, and Lewis Lung Carcinoma (LLC) from the American Type Culture Collection (ATCC), while A427 cells were obtained from Procell (Wuhan, China).

Techniques: Expressing, Incubation, Flow Cytometry, Cell Culture, Concentration Assay, Western Blot, Software

Journal: Cells

Article Title: Dauricine Impedes the Tumorigenesis of Lung Adenocarcinoma by Regulating Nrf2 and Reactive Oxygen Species

doi: 10.3390/cells14100698

Figure Lengend Snippet: Dauricine alters the expression of apoptosis-related proteins. ( A ) A549, ( C ) H1299, and ( E ) A427 cells were treated with 15 µM of dauricine prior to cell lysis and immunoblotting was performed with designated antibodies, and GAPDH blots display loading control. Column charts for ( B ) A549, ( D ) H1299, and ( F ) A427 represent the quantification of protein levels. All error bars represent the standard error of the mean (n = 3), and ** and *** indicating p < 0.01 and p < 0.001 respectively.

Article Snippet: Four non-small-cell lung cancer cells were procured: A549, H1299, and Lewis Lung Carcinoma (LLC) from the American Type Culture Collection (ATCC), while A427 cells were obtained from Procell (Wuhan, China).

Techniques: Expressing, Lysis, Western Blot, Control