Journal: International journal of cancer

Article Title: Human malignant glioma cell lines are sensitive to low radiation doses.

doi: 10.1002/ijc.11033

Figure Lengend Snippet: FIGURE 1 – Survival of human glioma cells following irradiation. Cells were irradiated with 0–2 Gy. For all experiments, all data are in sextuplicates. Errors bars represent SD. (a) G111, G142 and G152 glioma cell lines display HRS at doses below 1 Gy (p 0.05 for 0.7–0.9 or 0.8–1.0 Gy). (b) HRS was observed only with G5 cells (p 0.05 for 0.8–1.0 Gy), whereas its clone, CL35, displayed conventional sensitivity to radiation therapy. (c) HRS was observed with the radioresistant melanoma cell lines M4Be, A375P, MeWo and SKMel2 (p 0.05 for 0.7–0.9 Gy). (d) MRC5 human fibroblasts exhibit HRS (p 0.05 for 0.7–0.9 Gy). The radiation responses of the radiosensitive tumor cell lines H460 (lung cancer) and MCF7 (breast cancer) do not exhibit HRS but rather the LQ model.

Article Snippet: Four human melanoma cell lines were used: SKMel2, MeWo and A375P were obtained from the ATCC (Bethesda, MD) and M4Be was established in our laboratory.13 Cells were grown in McCoy’s 5A medium with L-glutamine supplemented with 10% FBS, which was heat-inactivated at 56°C for 30 min, 100 units/ml penicillin and 100 mg/ml streptomycin.

Techniques: Irradiation

Journal: Materials Today Bio

Article Title: Cyclic mechanical stretch suppresses intrinsic apoptosis in high metastatic melanoma

doi: 10.1016/j.mtbio.2026.103132

Figure Lengend Snippet: Cyclic mechanical strain engages the same anti-apoptotic pathway in high metastatic human melanoma as in the murine model. (A) Schematic of the experimental workflow translating the murine findings to human melanoma (B) Quantification of caspase-3/7-positive cells after 6 h of cyclic stretch. The apoptotic cell ratio was significantly reduced in the Stretch group compared to the static group in A375SM, whereas it showed a slight increase in A375P (n = 25). (C) Effect of BAX inhibition (BAI1) ± stretch. In A375SM, stretch alone lowers apoptosis more than BAI1 alone, and the combination produces an additional reduction (n = 25). (D) Effect of BCL-2 family inhibition (BH3I1) ± stretch. BH3I1 and stretch individually reduce apoptosis to a similar extent in A375SM, with no further decrease in the combined group (n = 25). (E) Effect of BAD inhibition (NPB) ± stretch. NPB and stretch each lower apoptosis in A375SM, and together yield the greatest suppression (n = 25). (F) Piezo1 inhibition (isoquercetin) raises apoptosis in A375SM irrespective of stretch, indicating a conserved mechano-protective role for Piezo1 (n = 25). (G) qPCR fold change in apoptosis-promoting genes (stretch/static). The response to these genes was blunted in A375SM compared to A375P (n = 3). (H) Fold change of anti-apoptotic genes is greater in A375SM than A375P (n = 3). (I) Fold change of proliferation markers is higher in A375SM, suggesting that caspase-3 activity may be redirected toward growth under mechanical stimulation (n = 3). ∗∗∗p < 0.0005; ∗∗p < 0.005; ∗p < 0.05, based on one-way ANOVA with Tukey HSD post-hoc testing. Error bars represent mean ± SD.

Article Snippet: B16F1, B16F10, A375P, and A375SM cell lines were acquired from the Korean Cell Line Bank.

Techniques: Inhibition, Activity Assay