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il18r1  (ABclonal Biotechnology)
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ABclonal Biotechnology il18r1
(A) Flow cytometry contour plots and quantification of Helios + Treg frequency among lung Tregs in WT and vimentin cKO mice after influenza infection. (B) Helios expression on lung Tregs of WT and vimentin cKO after influenza infection. (C) Helios + Treg number in whole lung after influenza infection. (D and G) Heatmap of differentially expressed genes ( p < 0.05). (E, F, H, I, and N) Normalized expression of Nt5e , Entpd1 , <t>Il18r1</t> , Areg , and Prkcq in Helios + and Helios − Tregs. (J) Frequency of Helios + IL-18R + , Helios + IL-18R − , and Helios − IL-18R + cells among lung Tregs after influenza infection. (K and L) Frequency and absolute number of Helios + IL-18R + Tregs in the whole lung tissue after influenza infection. (M) Relative mRNA expression of Il18r1 , Areg , and Vim in WT and Vim -deficient splenic nTregs. Data are mean ± SD, n = 4–5 mice/group. p values were calculated by two-way ANOVA (A–C and K–M) and two-tailed unpaired Student’s t test (E, F, H, I, and N). * p < 0.05, *** p < 0.0005, and **** p < 0.0001.
Il18r1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a2706/IL18R1+Rabbit+pAb/pmc11804169-454-24-25
Average 93 stars, based on 1 article reviews
il18r1 - by Bioz Stars, 2026-10
93/100 stars
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(A) Flow cytometry contour plots and quantification of Helios + Treg frequency among lung Tregs in WT and vimentin cKO mice after influenza infection. (B) Helios expression on lung Tregs of WT and vimentin cKO after influenza infection. (C) Helios + Treg number in whole lung after influenza infection. (D and G) Heatmap of differentially expressed genes ( p < 0.05). (E, F, H, I, and N) Normalized expression of Nt5e , Entpd1 , Il18r1 , Areg , and Prkcq in Helios + and Helios − Tregs. (J) Frequency of Helios + IL-18R + , Helios + IL-18R − , and Helios − IL-18R + cells among lung Tregs after influenza infection. (K and L) Frequency and absolute number of Helios + IL-18R + Tregs in the whole lung tissue after influenza infection. (M) Relative mRNA expression of Il18r1 , Areg , and Vim in WT and Vim -deficient splenic nTregs. Data are mean ± SD, n = 4–5 mice/group. p values were calculated by two-way ANOVA (A–C and K–M) and two-tailed unpaired Student’s t test (E, F, H, I, and N). * p < 0.05, *** p < 0.0005, and **** p < 0.0001.

Journal: Cell reports

Article Title: Vimentin modulates regulatory T cell receptor-ligand interactions at distal pole complex, leading to dysregulated host response to viral pneumonia

doi: 10.1016/j.celrep.2024.115056

Figure Lengend Snippet: (A) Flow cytometry contour plots and quantification of Helios + Treg frequency among lung Tregs in WT and vimentin cKO mice after influenza infection. (B) Helios expression on lung Tregs of WT and vimentin cKO after influenza infection. (C) Helios + Treg number in whole lung after influenza infection. (D and G) Heatmap of differentially expressed genes ( p < 0.05). (E, F, H, I, and N) Normalized expression of Nt5e , Entpd1 , Il18r1 , Areg , and Prkcq in Helios + and Helios − Tregs. (J) Frequency of Helios + IL-18R + , Helios + IL-18R − , and Helios − IL-18R + cells among lung Tregs after influenza infection. (K and L) Frequency and absolute number of Helios + IL-18R + Tregs in the whole lung tissue after influenza infection. (M) Relative mRNA expression of Il18r1 , Areg , and Vim in WT and Vim -deficient splenic nTregs. Data are mean ± SD, n = 4–5 mice/group. p values were calculated by two-way ANOVA (A–C and K–M) and two-tailed unpaired Student’s t test (E, F, H, I, and N). * p < 0.05, *** p < 0.0005, and **** p < 0.0001.

Article Snippet: Subsequently, cells were incubated with primary antibodies (vimentin [Invitrogen PA1–10003, 1:500]; Foxp3 [Novus Biologicals NB100–39002, 1:500]; CD43 [Invitrogen PA580067, 1:100]; ezrin [Abclonal A0703, 1:100]; IL18R1 [Abclonal A2706, 1:100]; CD73 [Invitrogen 41–0200, 1:50]; and PKC-θ [Cell Signaling Technologies 13643, 1:400]) either for 2 h at room temperature or overnight at 4°C.

Techniques: Flow Cytometry, Infection, Expressing, Two Tailed Test

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Vimentin modulates regulatory T cell receptor-ligand interactions at distal pole complex, leading to dysregulated host response to viral pneumonia

doi: 10.1016/j.celrep.2024.115056

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Subsequently, cells were incubated with primary antibodies (vimentin [Invitrogen PA1–10003, 1:500]; Foxp3 [Novus Biologicals NB100–39002, 1:500]; CD43 [Invitrogen PA580067, 1:100]; ezrin [Abclonal A0703, 1:100]; IL18R1 [Abclonal A2706, 1:100]; CD73 [Invitrogen 41–0200, 1:50]; and PKC-θ [Cell Signaling Technologies 13643, 1:400]) either for 2 h at room temperature or overnight at 4°C.

Techniques: Purification, Blocking Assay, Plasmid Preparation, Functional Assay, Control, Virus, Recombinant, Avidin-Biotin Assay, Cell Isolation, Enzyme-linked Immunosorbent Assay, Software