a260 Search Results


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  • 99
    Thermo Fisher a 260
    The effect of clozapine treatment on ATP production in 3T3-L1, C2C12, FL83B, and RAW264.7 cells. ATP levels were assessed using a bioluminescence assay in cells treated with 0, 25, 50 and 75 µM clozapine for 24 hours. Luminescence intensity corresponds to relative levels of ATP. Luminescence was corrected by using lysate <t>A260</t> values to correct for the number of viable cells contributing to ATP levels. P values shown are based on a Bonferroni post-hoc test.
    A 260, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a 260/product/Thermo Fisher
    Average 99 stars, based on 126 article reviews
    Price from $9.99 to $1999.99
    a 260 - by Bioz Stars, 2020-02
    99/100 stars
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    97
    Molecular Devices LLC a260
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 97/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a260/product/Molecular Devices LLC
    Average 97 stars, based on 76 article reviews
    Price from $9.99 to $1999.99
    a260 - by Bioz Stars, 2020-02
    97/100 stars
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    99
    Beckman Coulter a260
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 99/100, based on 226 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 226 article reviews
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    a260 - by Bioz Stars, 2020-02
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    99
    SAFAS a260 230
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260 230, supplied by SAFAS, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a260 230 - by Bioz Stars, 2020-02
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    94
    PerkinElmer a260 a280
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260 A280, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 21 article reviews
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    a260 a280 - by Bioz Stars, 2020-02
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    99
    Thermo Fisher a260 a230
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260 A230, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 485 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a260 a230/product/Thermo Fisher
    Average 99 stars, based on 485 article reviews
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    a260 a230 - by Bioz Stars, 2020-02
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    86
    Thermo Fisher a260 value
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260 Value, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a260 value - by Bioz Stars, 2020-02
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    99
    Thermo Fisher a260 230
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260 230, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 317 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a260 230 - by Bioz Stars, 2020-02
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    99
    Thermo Fisher a260 280nm
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260 280nm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a260 280nm - by Bioz Stars, 2020-02
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    77
    Thermo Fisher spectrophotometer a260
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    Spectrophotometer A260, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 77 stars, based on 4 article reviews
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    spectrophotometer a260 - by Bioz Stars, 2020-02
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    98
    GE Healthcare a260 280
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260 280, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 98/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 98 stars, based on 75 article reviews
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    a260 280 - by Bioz Stars, 2020-02
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    99
    Bio-Rad a260 280
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260 280, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a260 280/product/Bio-Rad
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    a260 280 - by Bioz Stars, 2020-02
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    99
    Eppendorf AG a260 a280
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260 A280, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a260 a280/product/Eppendorf AG
    Average 99 stars, based on 54 article reviews
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    a260 a280 - by Bioz Stars, 2020-02
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    99
    GE Healthcare a260 a280
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260 A280, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a260 a280/product/GE Healthcare
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    a260 a280 - by Bioz Stars, 2020-02
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    99
    Beckman Coulter a260 280
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260 280, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 99/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a260 280 - by Bioz Stars, 2020-02
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    94
    Biometra a260 a280
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260 A280, supplied by Biometra, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher a 260 a 280 ratio
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A 260 A 280 Ratio, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a 260 a 280 ratio - by Bioz Stars, 2020-02
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    99
    Bio-Rad a260 a280 ratio
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260 A280 Ratio, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a260 a280 ratio - by Bioz Stars, 2020-02
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    76
    Thermo Fisher a240 a260 ratio
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
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    Agilent technologies a260 280 ratios
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
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    BioSpec a260 a280 ratio
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
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    BioTek Instruments a260 a280 value
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
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    Molecular Devices LLC a260 a280 readings
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
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    Beckman Coulter a260 a280 ratio
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260 A280 Ratio, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 99/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Tecan Systems a260 a280 ratios
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
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    Thermo Fisher a260 a280 value
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260 A280 Value, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beckman Coulter spectrophotometer a260 a280
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
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    Agilent technologies a260
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
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    Bio-Rad a260
    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by <t>A260,</t> and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.
    A260, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Annealing patterns and Zn 2+ binding potentials of ssDNA composed of repetitive nucleotides. (a–d). EtBr-stained 2.5% agarose gels showing the annealing patterns of mononucleotide (a), dinucleotide (b), tri- or hexanucleoride repeats (c), and the ssDNA containing CG or TA-repeats (d). Note that although the equal amounts of DNA were loaded, some ssDNA controls (e.g., lanes 1 and 2 in a–c) were not (or only faintly) visible because these CG/GC sites-lacking DNA oligos are not expected to form stable helical structures (as those shown in Fig. 3 ) that are preferentially targeted by EtBr, a well-known double helical DNA intercalator. (e and f). <t>A295/A260</t> ratios plotted against metal ion concentrations as shown in Fig. 1 . (g) Potential Zn 2+ binding sites in the four types of nucleotides. The O and N atoms in the heterocyclic rings are highlighted in red. Hydrogen-bonding donors and receptors are indicated by gray and black arrows, respectively (adapted from ref. 10 ). Pink spheres representing the coordinated Zn 2+ -water complex (ref. 26 ). Note that adenine lacks O atom and the N3 atom of thymine may not be a preferred binding site for Zn 2+ (dashed lines) due to its bonding with the proton.
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    Image Search Results


    The effect of clozapine treatment on ATP production in 3T3-L1, C2C12, FL83B, and RAW264.7 cells. ATP levels were assessed using a bioluminescence assay in cells treated with 0, 25, 50 and 75 µM clozapine for 24 hours. Luminescence intensity corresponds to relative levels of ATP. Luminescence was corrected by using lysate A260 values to correct for the number of viable cells contributing to ATP levels. P values shown are based on a Bonferroni post-hoc test.

    Journal: PLoS ONE

    Article Title: Clozapine-Induced Mitochondria Alterations and Inflammation in Brain and Insulin-Responsive Cells

    doi: 10.1371/journal.pone.0059012

    Figure Lengend Snippet: The effect of clozapine treatment on ATP production in 3T3-L1, C2C12, FL83B, and RAW264.7 cells. ATP levels were assessed using a bioluminescence assay in cells treated with 0, 25, 50 and 75 µM clozapine for 24 hours. Luminescence intensity corresponds to relative levels of ATP. Luminescence was corrected by using lysate A260 values to correct for the number of viable cells contributing to ATP levels. P values shown are based on a Bonferroni post-hoc test.

    Article Snippet: ATP lysates were also quantified for A260 by NanoDrop (Thermo Scientific, Wilmington, DE, USA).

    Techniques: ATP Bioluminescent Assay

    The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by A260, and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.

    Journal: PLoS Pathogens

    Article Title: The Host Cell Sulfonation Pathway Contributes to Retroviral Infection at a Step Coincident with Provirus Establishment

    doi: 10.1371/journal.ppat.1000207

    Figure Lengend Snippet: The sulfonation pathway does not influence reverse transcription or the level of integrated virus DNA. (A and B) CHO-K1 cell lines were challenged with the VSV-G pseudotyped MLV vector LEGFP. Total DNA (A) or DNA from isolated nuclei (B) were harvested at 0 or 24 hpi, DNA concentration was quantitated by A260, and a real-time PCR amplification analysis was performed to measure the levels of viral DNA that were synthesized. (C) Untreated CHO-K1 cells or CHO-K1 cells that had been pretreated for 16 hrs with 100 mM chlorate and maintained in medium containing this concentration of chlorate were challenged with the MLV vector and subsequently analyzed for reverse transcription products as described in panel A. (D) CHO-K1 cells that were either untreated or treated with 100 mM chlorate, IM2 cells, and MCL7 cells, were challenged with the same VSV-G pseudotyped MLV vector and total DNA was harvested at 1 or 18 days post infection for real time PCR quantitation of viral DNA products. The chemically-mutagenized MCL7 cell line displays a strong block to MLV DNA integration [39] . Chlorate treated CHO-K1 cells were passaged in medium containing 100 mM chlorate for the duration of the experiment. The data shown in panels A–D are the average mean values obtained in independent experiments performed with triplicate samples and each is representative of three independent experiments. Error bars indicate the standard deviation of the data.

    Article Snippet: DNA concentration was calculated by measuring the A260 on a SPECTRAmax Plus 96 well UV spectrophotometer (Molecular Devices, Sunnyvale, CA).

    Techniques: Plasmid Preparation, Isolation, Concentration Assay, Real-time Polymerase Chain Reaction, Amplification, Synthesized, Infection, Quantitation Assay, Blocking Assay, Standard Deviation

    Annealing patterns and Zn 2+ binding potentials of ssDNA composed of repetitive nucleotides. (a–d). EtBr-stained 2.5% agarose gels showing the annealing patterns of mononucleotide (a), dinucleotide (b), tri- or hexanucleoride repeats (c), and the ssDNA containing CG or TA-repeats (d). Note that although the equal amounts of DNA were loaded, some ssDNA controls (e.g., lanes 1 and 2 in a–c) were not (or only faintly) visible because these CG/GC sites-lacking DNA oligos are not expected to form stable helical structures (as those shown in Fig. 3 ) that are preferentially targeted by EtBr, a well-known double helical DNA intercalator. (e and f). A295/A260 ratios plotted against metal ion concentrations as shown in Fig. 1 . (g) Potential Zn 2+ binding sites in the four types of nucleotides. The O and N atoms in the heterocyclic rings are highlighted in red. Hydrogen-bonding donors and receptors are indicated by gray and black arrows, respectively (adapted from ref. 10 ). Pink spheres representing the coordinated Zn 2+ -water complex (ref. 26 ). Note that adenine lacks O atom and the N3 atom of thymine may not be a preferred binding site for Zn 2+ (dashed lines) due to its bonding with the proton.

    Journal: Scientific Reports

    Article Title: Zn2+ Blocks Annealing of Complementary Single-Stranded DNA in a Sequence-Selective Manner

    doi: 10.1038/srep05464

    Figure Lengend Snippet: Annealing patterns and Zn 2+ binding potentials of ssDNA composed of repetitive nucleotides. (a–d). EtBr-stained 2.5% agarose gels showing the annealing patterns of mononucleotide (a), dinucleotide (b), tri- or hexanucleoride repeats (c), and the ssDNA containing CG or TA-repeats (d). Note that although the equal amounts of DNA were loaded, some ssDNA controls (e.g., lanes 1 and 2 in a–c) were not (or only faintly) visible because these CG/GC sites-lacking DNA oligos are not expected to form stable helical structures (as those shown in Fig. 3 ) that are preferentially targeted by EtBr, a well-known double helical DNA intercalator. (e and f). A295/A260 ratios plotted against metal ion concentrations as shown in Fig. 1 . (g) Potential Zn 2+ binding sites in the four types of nucleotides. The O and N atoms in the heterocyclic rings are highlighted in red. Hydrogen-bonding donors and receptors are indicated by gray and black arrows, respectively (adapted from ref. 10 ). Pink spheres representing the coordinated Zn 2+ -water complex (ref. 26 ). Note that adenine lacks O atom and the N3 atom of thymine may not be a preferred binding site for Zn 2+ (dashed lines) due to its bonding with the proton.

    Article Snippet: UV-Vis spectrum analysis Aliquots (100 µl) of solutions (10 mM NaCl, 10 mM Tris pH 7.5) containing ssDNA (1 µm) and metal ions (0–750 µm) or metal ion (750 µm) plus EDTA (1 mM) (control) were incubated in a 96-well clear microtiter plate (Corning) at 37°C for 10 min before scanning for absorbance at 260 nm (A260) and 295 nm (A295) using a Synergy H1 microplate reader (BioTek) with default settings for pathlength correction.

    Techniques: Binding Assay, Staining

    Zn 2+ blocks annealing of complementary ssDNA in a sequence-selective manner. (a) LTEAGE procedure. (b) Ethidium bromide (EtBr)-stained 2.5% agarose gels showing the annealing patterns. Control lanes were loaded with ssDNA (+ or – strand) without Zn 2+ treatment. Lanes 9 were loaded with EDTA-treated (1 mM) samples. Double/single-stranded DNA-specific bands are indicated by black and gray arrows, respectively. M: 50-bp DNA ladder; (c) A295/A260 ratios plotted against metal ion concentrations. Arrows indicate the first points at which the ratio increased significantly. Numbers on the right of the strand symbols indicate the corresponding SD Z values (at 250 µm). (d) and (e) Fluorescence quenching assay. Bars in (c) and (e) indicate standard deviations, most of which in (c) are masked by the squares representing the means; asterisks indicate significant differences ( t -test, P

    Journal: Scientific Reports

    Article Title: Zn2+ Blocks Annealing of Complementary Single-Stranded DNA in a Sequence-Selective Manner

    doi: 10.1038/srep05464

    Figure Lengend Snippet: Zn 2+ blocks annealing of complementary ssDNA in a sequence-selective manner. (a) LTEAGE procedure. (b) Ethidium bromide (EtBr)-stained 2.5% agarose gels showing the annealing patterns. Control lanes were loaded with ssDNA (+ or – strand) without Zn 2+ treatment. Lanes 9 were loaded with EDTA-treated (1 mM) samples. Double/single-stranded DNA-specific bands are indicated by black and gray arrows, respectively. M: 50-bp DNA ladder; (c) A295/A260 ratios plotted against metal ion concentrations. Arrows indicate the first points at which the ratio increased significantly. Numbers on the right of the strand symbols indicate the corresponding SD Z values (at 250 µm). (d) and (e) Fluorescence quenching assay. Bars in (c) and (e) indicate standard deviations, most of which in (c) are masked by the squares representing the means; asterisks indicate significant differences ( t -test, P

    Article Snippet: UV-Vis spectrum analysis Aliquots (100 µl) of solutions (10 mM NaCl, 10 mM Tris pH 7.5) containing ssDNA (1 µm) and metal ions (0–750 µm) or metal ion (750 µm) plus EDTA (1 mM) (control) were incubated in a 96-well clear microtiter plate (Corning) at 37°C for 10 min before scanning for absorbance at 260 nm (A260) and 295 nm (A295) using a Synergy H1 microplate reader (BioTek) with default settings for pathlength correction.

    Techniques: Sequencing, Staining, Fluorescence