Journal: Advanced Science

Article Title: Vinburnine Sensitizes Radiotherapy Efficacy in Nasopharyngeal Carcinoma by Triggering Pyroptosis and Immune Responses via Activation of EDAR‐NFκB Pathway

doi: 10.1002/advs.202506139

Figure Lengend Snippet: Vinburnine promotes IR‐EDAR‐NFκB‐induced apoptosis and pyroptosis. A) Reactome analysis of upregulated differential genes after 5µ m Vin / 2Gy IR treatment for 48h. B) Heatmap of differential gene expression in transcriptomics. C) qPCR of EDAR mRNA in NPC cells treated with 5µ m Vin / 2Gy IR for 48h ( n = 3). D) Protein expression of EDAR in the cell membrane/cytoplasm after being treated with 5µ m Vin / 2Gy IR for 48h. E) Western blotting detected the protein expression levels of the NFκB pathway (p65/p50) and pyroptosis index (GSDME/N‐GSDME/ Cleaved‐Caspase3) after 5µ m Vin / 2Gy IR treatment for 48h. Upon activation of the NFκB signaling pathway, its downstream signal, Caspase3, undergoes cleavage. This cleavage then cuts GSDME to form N‐GSDME, ultimately resulting in the pyroptosis of the cells. F) The binding of p65 to the EDAR promoter in the treated SUNE1 cells was detected by ChIP assay. G) The CB‐Dock2 website predicts the structural complex of vinburnine bound with the EDAR protein. Vinburnine is colored green; EDAR is colored grey and yellow. H) SPR technology proved that EDAR is the target of vinburnine. I) The Co‐IP experiment confirmed that after treatment with 5µ m Vin+2Gy IR, EDAR formed more protein complexes with EDARADD/TRAF6. Multiple samples were presented using mean ± standard deviation (SD). C) Statistical analysis with Two‐way ANOVA was used to analyze the statistical differences among multiple groups. F) Statistical analysis with One‐way ANOVA was used to analyze the statistical differences among multiple groups. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns for non‐significant.

Article Snippet: Subsequently, western blotting confirmed that EDAR (Proteintech, China) formed protein complexes with EDARADD (Abclone, China) and TRAF6 (Immunoway, USA).

Techniques: Gene Expression, Expressing, Membrane, Western Blot, Activation Assay, Binding Assay, Co-Immunoprecipitation Assay, Standard Deviation

Journal: Advanced Science

Article Title: Vinburnine Sensitizes Radiotherapy Efficacy in Nasopharyngeal Carcinoma by Triggering Pyroptosis and Immune Responses via Activation of EDAR‐NFκB Pathway

doi: 10.1002/advs.202506139

Figure Lengend Snippet: Knocking down EDAR suppresses the radiosensitization effect of vinburnine. A) The cytotoxic effect of 5uM Vin/2Gy IR on EDAR‐knockdown cells was assessed using the CCK‐8 assay ( n = 5). B) Following EDAR knockdown, cells were treated with 5uM Vin/2Gy IR. Colony formation was evaluated by crystal violet staining (left) and the number of colonies was quantified (right) ( n = 3). C–E) Flow cytometry detected the apoptosis/ROS levels/mitochondrial membrane potential of the Vin±IR‐treated cells after EDAR knockdown ( n = 3). F) After EDAR knockdown, western blotting detected the protein expression (p65/p50/GSDME/N‐GSDME/Cleaved‐Caspase3) in the treated group. Multiple samples were presented using mean ± standard deviation (SD). A–E) Statistical analysis with Two‐way ANOVA was used to analyze the statistical differences among multiple groups. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns for non‐significant.

Article Snippet: Subsequently, western blotting confirmed that EDAR (Proteintech, China) formed protein complexes with EDARADD (Abclone, China) and TRAF6 (Immunoway, USA).

Techniques: Knockdown, CCK-8 Assay, Staining, Flow Cytometry, Membrane, Western Blot, Expressing, Standard Deviation

Journal: ASN NEURO

Article Title: Microglial- and Astrocyte-Specific Expression of Purinergic Signaling Components and Inflammatory Mediators in the Rat Hippocampus During Trimethyltin-Induced Neurodegeneration

doi: 10.1177/17590914211044882

Figure Lengend Snippet: List of Antibodies.

Article Snippet: A 1 R , Rabbit, polyclonal , 1:200 IF , Novus Biologicals, NB300-549, RRID: AB_10002337.

Techniques: Immunohistochemistry-IF