Journal: Viruses

Article Title: Histone Deacetylase Inhibitor Panobinostat Benefits the Therapeutic Efficacy of Oncolytic Herpes Simplex Virus Combined with PD-1/PD-L1 Blocking in Glioma and Squamous Cell Carcinoma Models

doi: 10.3390/v14122796

Figure Lengend Snippet: Panobinostat treatment facilitated oHSV replication and tumor cytotoxicity in human squamous cell carcinoma SCC9 cells. Human squamous cell carcinoma SCC9 ( A , B ) and Tca8113 cells ( C , D ) were pretreated with panobinostat (1, 10, or 100 nM) for 14 h and then infected with T1012G (0.1 or 1 PFU/Cell) for another 48 h, respectively. The infected cell pellets were harvested at 48 hpi. The titration was measured by conventional plaque assay on Vero cells after three freeze–thaw cycles. The cytotoxicity activity was assessed using a CCK8 assay. SCC9 ( E ) and Tca8113 ( F ) cells were mock- or pretreated with panobinostat as above, following infection with T1012G for 48 h. The cell viability of the infected cells was measured with a CCK8 assay (mean ± SD). The assay was performed in triplicate. Differences between datasets were assessed by Student’s t -test (two-tailed) using GraphPad Prism software. * p < 0.05 and ** p < 0.01.

Article Snippet: A172, D54, and Tca8113 cells were purchased from JOINN Biologics, SCC9 cells were purchased from the BeNa Culture Collection, and CT2A cells were purchased from BLUEFBIO.

Techniques: Infection, Titration, Plaque Assay, Activity Assay, CCK-8 Assay, Two Tailed Test, Software

Journal: Viruses

Article Title: Histone Deacetylase Inhibitor Panobinostat Benefits the Therapeutic Efficacy of Oncolytic Herpes Simplex Virus Combined with PD-1/PD-L1 Blocking in Glioma and Squamous Cell Carcinoma Models

doi: 10.3390/v14122796

Figure Lengend Snippet: Downregulation of IFN-β- and IFN-stimulated antiviral genes when treated with panobinostat in human glioma and squamous cell carcinoma cells. A172 ( A1 , B1 , C1 , D1 ), D54 ( A2 , B2 , C2 , D2 ), SCC9 ( E1 , F1 , G1 , H1 ), or Tca8113 ( E2 , F2 , G2 , H2 ) cells were mock-treated or treated with panobinostat at different concentrations of 1, 10, or 100 nM for 14 h, respectively. Then, the cell pellets were harvested and total RNAs were extracted and reverse-transcribed to cDNA as described in the Materials and Methods section. The IFN-β, STAT1, PKR, and PML mRNAs were quantified and normalized with respect to 18S rRNA and are shown as fold changes compared to the mRNAs from the mock-treated and infected cells. Differences between datasets were assessed by Student’s t -test (two-tailed) using GraphPad Prism software. NS p >0.05, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: A172, D54, and Tca8113 cells were purchased from JOINN Biologics, SCC9 cells were purchased from the BeNa Culture Collection, and CT2A cells were purchased from BLUEFBIO.

Techniques: Reverse Transcription, Infection, Two Tailed Test, Software

Journal: Viruses

Article Title: Histone Deacetylase Inhibitor Panobinostat Benefits the Therapeutic Efficacy of Oncolytic Herpes Simplex Virus Combined with PD-1/PD-L1 Blocking in Glioma and Squamous Cell Carcinoma Models

doi: 10.3390/v14122796

Figure Lengend Snippet: Suppression of the transcriptional expression of cGAS/STING in human glioma and squamous cell carcinoma cells when treated with panobinostat. A172 ( A1 , B1 , C1 , D1 ), D54 ( A2 , B2 , C2 , D2 ), SCC9 ( E1 , F1 , G1 , H1 ), or Tca8113 ( E2 , F2 , G2 , H2 ) cells were mock-treated or treated with panobinostat at different concentrations of 1, 10, or 100 nM for 14 h, respectively. Then, the cell pellets were harvested and total RNAs were extracted and reverse-transcribed to cDNA as described in the Materials and Methods section. cGAS, STING, TBK1, and IRF3 were quantified and normalized with respect to 18S rRNA and shown as fold changes. Differences between datasets were assessed by Student’s t -test (two-tailed) using GraphPad Prism software. * p < 0.05, ** p < 0.01.

Article Snippet: A172, D54, and Tca8113 cells were purchased from JOINN Biologics, SCC9 cells were purchased from the BeNa Culture Collection, and CT2A cells were purchased from BLUEFBIO.

Techniques: Expressing, Reverse Transcription, Two Tailed Test, Software

Journal: Viruses

Article Title: Histone Deacetylase Inhibitor Panobinostat Benefits the Therapeutic Efficacy of Oncolytic Herpes Simplex Virus Combined with PD-1/PD-L1 Blocking in Glioma and Squamous Cell Carcinoma Models

doi: 10.3390/v14122796

Figure Lengend Snippet: Panobinostat treatment upregulated the PD-L1 expression in glioma and squamous cell carcinoma cells. A172 ( A , B ), SCC9 ( C , D ), D54 ( E ), Tca8113 ( F ), CT-2A ( G ), or SCC7 ( H ) cells were mock-treated or treated with panobinostat at different concentrations of 1, 10, or 100 nM for 14 h, respectively. Then, the cell pellets were harvested. For each condition, half of the cell pellets were used for total RNA extraction, following reverse transcription to cDNA. The other half of the cell pellets were lysed and used for Western blot (WB) as described in the Materials and Methods section. The assay was performed in triplicate. Differences between datasets were assessed by Student’s t -test (two-tailed) using GraphPad Prism software. * p < 0.05, ** p < 0.01.

Article Snippet: A172, D54, and Tca8113 cells were purchased from JOINN Biologics, SCC9 cells were purchased from the BeNa Culture Collection, and CT2A cells were purchased from BLUEFBIO.

Techniques: Expressing, RNA Extraction, Reverse Transcription, Western Blot, Two Tailed Test, Software