VR-1564AS-Gt Search Results


goat antiserum against rauscher murine leukemia virus p30  (ATCC)
92
ATCC goat antiserum against rauscher murine leukemia virus p30
Generation of CAR T cells. a Schematic representation of the CAR constructs containing an antigen recognition domain (scFv or DARPin), IgG1 hinge-CH2/CH3 domain, CD28 transmembrane- and intracellular domain, intracellular CD3ζ domain. nc-DARPin is of irrelevant specificity and served as control. b Presence of <t>MLV-gag</t> protein <t>p30</t> as part of γ-retroviral vectors in supernatant of transfected HEK293T/17 cells as detected by Western blot analysis using and MLV p30 specific antibody: mock (lane2) or CAR delivering vector particles (lanes 3–5), supernatant of non-transfected cells served as negative control (lane 1). c CAR expression on the surface of CD3 + human T cells as recorded by flow cytometry, 3 days post-transduction. d Transduction efficiency (left) and Median Fluorescence Intensity (MFI, right) of CD4-specific scFv CARs A8, A12, C1, G2 and DARPin CAR by CD8 + T cells of the unrelated donors D24 and D25 as assessed by flow cytometry 12 h post-transduction
Goat Antiserum Against Rauscher Murine Leukemia Virus P30, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat antiserum against rauscher murine leukemia virus p30/product/ATCC
Average 92 stars, based on 1 article reviews
goat antiserum against rauscher murine leukemia virus p30 - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier
vr-1564as-gt  (LGC Standards)
91
LGC Standards vr-1564as-gt
Generation of CAR T cells. a Schematic representation of the CAR constructs containing an antigen recognition domain (scFv or DARPin), IgG1 hinge-CH2/CH3 domain, CD28 transmembrane- and intracellular domain, intracellular CD3ζ domain. nc-DARPin is of irrelevant specificity and served as control. b Presence of <t>MLV-gag</t> protein <t>p30</t> as part of γ-retroviral vectors in supernatant of transfected HEK293T/17 cells as detected by Western blot analysis using and MLV p30 specific antibody: mock (lane2) or CAR delivering vector particles (lanes 3–5), supernatant of non-transfected cells served as negative control (lane 1). c CAR expression on the surface of CD3 + human T cells as recorded by flow cytometry, 3 days post-transduction. d Transduction efficiency (left) and Median Fluorescence Intensity (MFI, right) of CD4-specific scFv CARs A8, A12, C1, G2 and DARPin CAR by CD8 + T cells of the unrelated donors D24 and D25 as assessed by flow cytometry 12 h post-transduction
Vr 1564as Gt, supplied by LGC Standards, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vr-1564as-gt/product/LGC Standards
Average 91 stars, based on 1 article reviews
vr-1564as-gt - by Bioz Stars, 2026-02
91/100 stars
  Buy from Supplier

Image Search Results


Generation of CAR T cells. a Schematic representation of the CAR constructs containing an antigen recognition domain (scFv or DARPin), IgG1 hinge-CH2/CH3 domain, CD28 transmembrane- and intracellular domain, intracellular CD3ζ domain. nc-DARPin is of irrelevant specificity and served as control. b Presence of MLV-gag protein p30 as part of γ-retroviral vectors in supernatant of transfected HEK293T/17 cells as detected by Western blot analysis using and MLV p30 specific antibody: mock (lane2) or CAR delivering vector particles (lanes 3–5), supernatant of non-transfected cells served as negative control (lane 1). c CAR expression on the surface of CD3 + human T cells as recorded by flow cytometry, 3 days post-transduction. d Transduction efficiency (left) and Median Fluorescence Intensity (MFI, right) of CD4-specific scFv CARs A8, A12, C1, G2 and DARPin CAR by CD8 + T cells of the unrelated donors D24 and D25 as assessed by flow cytometry 12 h post-transduction

Journal: Medical Microbiology and Immunology

Article Title: Designed Ankyrin Repeat Protein (DARPin) to target chimeric antigen receptor (CAR)-redirected T cells towards CD4 + T cells to reduce the latent HIV + cell reservoir

doi: 10.1007/s00430-020-00692-0

Figure Lengend Snippet: Generation of CAR T cells. a Schematic representation of the CAR constructs containing an antigen recognition domain (scFv or DARPin), IgG1 hinge-CH2/CH3 domain, CD28 transmembrane- and intracellular domain, intracellular CD3ζ domain. nc-DARPin is of irrelevant specificity and served as control. b Presence of MLV-gag protein p30 as part of γ-retroviral vectors in supernatant of transfected HEK293T/17 cells as detected by Western blot analysis using and MLV p30 specific antibody: mock (lane2) or CAR delivering vector particles (lanes 3–5), supernatant of non-transfected cells served as negative control (lane 1). c CAR expression on the surface of CD3 + human T cells as recorded by flow cytometry, 3 days post-transduction. d Transduction efficiency (left) and Median Fluorescence Intensity (MFI, right) of CD4-specific scFv CARs A8, A12, C1, G2 and DARPin CAR by CD8 + T cells of the unrelated donors D24 and D25 as assessed by flow cytometry 12 h post-transduction

Article Snippet: Production of retroviral particles was confirmed by Western blot using goat antiserum against Rauscher Murine Leukemia Virus p30 (ATCC, VR1564ASGt) as detection antibody.

Techniques: Construct, Control, Retroviral, Transfection, Western Blot, Plasmid Preparation, Negative Control, Expressing, Flow Cytometry, Transduction, Fluorescence

Killing of autologous CD4 + T cells. a Composition of T cell subsets from peripheral blood after incubation with autologous anti-CD4-DARPin CAR T cells. CD3 + T cells were analyzed by flow cytometry for the presence of CD4 (black) and CD8 (grey) on days 0, 3, 5 and 8 post-transduction with MLV-based vectors encoding the anti-CD30scFv CAR, anti-CD4-DARPin CAR or with empty vectors (mock). Data represent the standard deviation of the mean (SD), n = 6 donors, three independent experiments. b Counts in % of total of autologous CD4 + target T cells after 12 h co-culture with CD8 + T cells engineered with the CD4-specific scFv or DARPin CAR T effector cells. Data were assessed by flow cytometry showing mean SD of technical triplicates. For normalization, CD8 + T cell counts were set to 100% for each sample. Error bars represent mean SD of biological triplicates. Statistical significance compared to untransduced sample (UT) is shown by unpaired student’s t test for donors D24 (left; * p ≤ 0.0001, ** p ≤ 0.00001) and D25 (right; * p ≤ 0.03, ** p ≤ 0.003)

Journal: Medical Microbiology and Immunology

Article Title: Designed Ankyrin Repeat Protein (DARPin) to target chimeric antigen receptor (CAR)-redirected T cells towards CD4 + T cells to reduce the latent HIV + cell reservoir

doi: 10.1007/s00430-020-00692-0

Figure Lengend Snippet: Killing of autologous CD4 + T cells. a Composition of T cell subsets from peripheral blood after incubation with autologous anti-CD4-DARPin CAR T cells. CD3 + T cells were analyzed by flow cytometry for the presence of CD4 (black) and CD8 (grey) on days 0, 3, 5 and 8 post-transduction with MLV-based vectors encoding the anti-CD30scFv CAR, anti-CD4-DARPin CAR or with empty vectors (mock). Data represent the standard deviation of the mean (SD), n = 6 donors, three independent experiments. b Counts in % of total of autologous CD4 + target T cells after 12 h co-culture with CD8 + T cells engineered with the CD4-specific scFv or DARPin CAR T effector cells. Data were assessed by flow cytometry showing mean SD of technical triplicates. For normalization, CD8 + T cell counts were set to 100% for each sample. Error bars represent mean SD of biological triplicates. Statistical significance compared to untransduced sample (UT) is shown by unpaired student’s t test for donors D24 (left; * p ≤ 0.0001, ** p ≤ 0.00001) and D25 (right; * p ≤ 0.03, ** p ≤ 0.003)

Article Snippet: Production of retroviral particles was confirmed by Western blot using goat antiserum against Rauscher Murine Leukemia Virus p30 (ATCC, VR1564ASGt) as detection antibody.

Techniques: Incubation, Flow Cytometry, Transduction, Standard Deviation, Co-Culture Assay