Journal: Nature Communications
Article Title: Quantitative evaluation of protective antibody response induced by hepatitis E vaccine in humans
Figure Lengend Snippet: Study design and clinical measures of HEV vaccine response. a Immunization regimen and critical time points in the longitudinal analysis of human B-cell response to the HEV vaccine, Hecolin ® . The first, second, and third doses are indicated above the time axis at days 0, 31, and 183, respectively, with black arrows and needles. The seven time points selected for antibody-repertoire sequencing are indicated below the time axis with red arrows and “NGS.” Vaccine-induced, p239(1)-specific monoclonal antibodies (mAbs) were isolated from samples collected 1 month after the third dose (3M1), which is labeled below the time axis. Dynamics of anti-HEV IgG titer ( b ), anti-HEV IgG avidity ( c ), and serum neutralization measured by the 50% inhibitory dose (ID 50 ) ( d ) during vaccination. Error bars indicate standard deviation (s.d.) in ( b ). The dash lines represent the detection limit for the anti-HEV IgG and the threshold of neutralizing capacity for the serum neutralization assays. *Only anti-HEV IgG positive sera were tested for anti-HEV IgG avidity. Source data are provided in the Source Data file.
Article Snippet: The membranes were blocked with 5% skim milk and then incubated with sera or p239(1)-specific mAbs for 30 min. After rinse, the membranes were incubated with anti-human HRP-conjugated antibody (Sino Biological inc, Cat# SSA001).
Techniques: Sequencing, Isolation, Labeling, Neutralization, Standard Deviation