SPC-506 Search Results


a11 oligomers  (StressMarq)
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StressMarq a11 oligomers
( a-c ) Recombinant purified myc-αSyn was shaken at 37°C in presence of concentrated extracellular medium from mouse cortical neuron cultures collected over 7 days (DIV 42-49), generated either from wild type (WT) mice, or from Tg x2 -αSyn A53T mice with or without lentiviral expression of VAMP7 dominant-negative fragment (VAMP7 DN ; infected at DIV 7). Aggregation of myc-αSyn was analyzed at the indicated days of incubation by the following assays: ( a ) Congo-red derivative, amyloid-binding dye K114 fluorescence at 390/535 nm (n=4). ( b ) Amyloid-binding dye Thioflavin-T fluorescence at 450/485 nm (n=4). ( c ) Quantitative immunoblotting for the myc epitope-tag, where aggregation is measured as disappearance of monomeric myc-αSyn (top; n=4); dot-blotting for filamentous myc-αSyn aggregates using αSyn Fila antibody (middle; n=4); and dot-blotting for amyloid-type myc-αSyn aggregates using αSyn Amyl <t>A11</t> antibody (bottom; n=4). All data represent means ± SEM, where each ‘n’ is an independent aggregation experiment. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 by RM 2-way ANOVA.
A11 Oligomers, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/SPC-506/Anti-Amyloid+Oligomers+(A11)+Antibody/bio_rxiv__2021__04__10__439302-212-3-5
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a11 oligomers - by Bioz Stars, 2026-10
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( a-c ) Recombinant purified myc-αSyn was shaken at 37°C in presence of concentrated extracellular medium from mouse cortical neuron cultures collected over 7 days (DIV 42-49), generated either from wild type (WT) mice, or from Tg x2 -αSyn A53T mice with or without lentiviral expression of VAMP7 dominant-negative fragment (VAMP7 DN ; infected at DIV 7). Aggregation of myc-αSyn was analyzed at the indicated days of incubation by the following assays: ( a ) Congo-red derivative, amyloid-binding dye K114 fluorescence at 390/535 nm (n=4). ( b ) Amyloid-binding dye Thioflavin-T fluorescence at 450/485 nm (n=4). ( c ) Quantitative immunoblotting for the myc epitope-tag, where aggregation is measured as disappearance of monomeric myc-αSyn (top; n=4); dot-blotting for filamentous myc-αSyn aggregates using αSyn Fila antibody (middle; n=4); and dot-blotting for amyloid-type myc-αSyn aggregates using αSyn Amyl A11 antibody (bottom; n=4). All data represent means ± SEM, where each ‘n’ is an independent aggregation experiment. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 by RM 2-way ANOVA.

Journal: bioRxiv

Article Title: Lysosomal Exocytosis Releases Pathogenic α-Synuclein Species from Neurons

doi: 10.1101/2021.04.10.439302

Figure Lengend Snippet: ( a-c ) Recombinant purified myc-αSyn was shaken at 37°C in presence of concentrated extracellular medium from mouse cortical neuron cultures collected over 7 days (DIV 42-49), generated either from wild type (WT) mice, or from Tg x2 -αSyn A53T mice with or without lentiviral expression of VAMP7 dominant-negative fragment (VAMP7 DN ; infected at DIV 7). Aggregation of myc-αSyn was analyzed at the indicated days of incubation by the following assays: ( a ) Congo-red derivative, amyloid-binding dye K114 fluorescence at 390/535 nm (n=4). ( b ) Amyloid-binding dye Thioflavin-T fluorescence at 450/485 nm (n=4). ( c ) Quantitative immunoblotting for the myc epitope-tag, where aggregation is measured as disappearance of monomeric myc-αSyn (top; n=4); dot-blotting for filamentous myc-αSyn aggregates using αSyn Fila antibody (middle; n=4); and dot-blotting for amyloid-type myc-αSyn aggregates using αSyn Amyl A11 antibody (bottom; n=4). All data represent means ± SEM, where each ‘n’ is an independent aggregation experiment. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 by RM 2-way ANOVA.

Article Snippet: β-Actin: Sigma (A1978); A11 oligomers: Stressmarq (SPC-506D); ATP5G: Abcam (ab181243); Calreticulin: Thermo Fisher (OTI15F5) and Novus (NB600-103); Cathepsin-L: Novus (JM10-78); CD81: Novus (SN206-01); EEA1: Thermo Fisher (MA5-14794); Flag: Sigma Cl.

Techniques: Recombinant, Purification, Generated, Expressing, Dominant Negative Mutation, Infection, Incubation, Binding Assay, Fluorescence, Western Blot