SMC-346 Search Results


iv mouse anti gaba a α1 subunit  (StressMarq)
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StressMarq iv mouse anti gaba a α1 subunit
Increased hippocampal levels of <t>GABA</t> <t>A</t> receptor <t>α1</t> subunit correlate with flurothyl seizure susceptibility. Shown are representative confocal images (20×/0.8 objective) of hippocampal pyramidal (Py) and oriens (Or) layers for PV (Alexa 488, green; A 1 ), somatostatin (SST, Alexa 568, red; A 2 ), GABA A receptor α1 subunit (GABA A Rα1, Alexa 647, magenta; A 3 ), and merged (A 4 ) with nuclear DAPI (blue) in sham, normothermia (NT), and therapeutic hypothermia (TH) mice. Box and whisker plots demonstrate the percent expression of GABA A Rα1 in each group (B 1 ) and the correlations with the number of PV + interneurons (INs) in the CA1 region (B 2 ). In some instances, GABA A Rα1 immunoreactivity was extreme despite blocking methods (C a ) and absent of non-specific staining in negative controls (C b ). Correlations between GABA A Rα1 and hippocampal (Hp) injury scores (D), Hp volume measurements (E), and latency to stage 5 (S5) seizures with flurothyl (F) are shown. Adjustment of the number of PV + INs by their relative expression of GABA A Rα1 affects the correlation with latency to S5 seizures with flurothyl (G), now becoming linear. Boxes are limited by the 25th and 75th percentiles (interquartile range, IQR) and whiskers are limited by the last data point within 1.5 times the IQR from the median (continuous line inside the box), with outliers included. Kruskal–Wallis ANOVA with Dunn-Bonferoni post-hoc testing for pair analysis was applied. *, p < 0.05. In correlation charts, the continuous lines represent the fitted line derived from regression modeling and the discontinuous lines represent the 95% confidence boundries.
Iv Mouse Anti Gaba A α1 Subunit, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Increased hippocampal levels of GABA A receptor α1 subunit correlate with flurothyl seizure susceptibility. Shown are representative confocal images (20×/0.8 objective) of hippocampal pyramidal (Py) and oriens (Or) layers for PV (Alexa 488, green; A 1 ), somatostatin (SST, Alexa 568, red; A 2 ), GABA A receptor α1 subunit (GABA A Rα1, Alexa 647, magenta; A 3 ), and merged (A 4 ) with nuclear DAPI (blue) in sham, normothermia (NT), and therapeutic hypothermia (TH) mice. Box and whisker plots demonstrate the percent expression of GABA A Rα1 in each group (B 1 ) and the correlations with the number of PV + interneurons (INs) in the CA1 region (B 2 ). In some instances, GABA A Rα1 immunoreactivity was extreme despite blocking methods (C a ) and absent of non-specific staining in negative controls (C b ). Correlations between GABA A Rα1 and hippocampal (Hp) injury scores (D), Hp volume measurements (E), and latency to stage 5 (S5) seizures with flurothyl (F) are shown. Adjustment of the number of PV + INs by their relative expression of GABA A Rα1 affects the correlation with latency to S5 seizures with flurothyl (G), now becoming linear. Boxes are limited by the 25th and 75th percentiles (interquartile range, IQR) and whiskers are limited by the last data point within 1.5 times the IQR from the median (continuous line inside the box), with outliers included. Kruskal–Wallis ANOVA with Dunn-Bonferoni post-hoc testing for pair analysis was applied. *, p < 0.05. In correlation charts, the continuous lines represent the fitted line derived from regression modeling and the discontinuous lines represent the 95% confidence boundries.

Journal: Neurobiology of disease

Article Title: Sex specific correlation between GABAergic disruption in the dorsal hippocampus and flurothyl seizure susceptibility after neonatal hypoxic-ischemic brain injury

doi: 10.1016/j.nbd.2020.105222

Figure Lengend Snippet: Increased hippocampal levels of GABA A receptor α1 subunit correlate with flurothyl seizure susceptibility. Shown are representative confocal images (20×/0.8 objective) of hippocampal pyramidal (Py) and oriens (Or) layers for PV (Alexa 488, green; A 1 ), somatostatin (SST, Alexa 568, red; A 2 ), GABA A receptor α1 subunit (GABA A Rα1, Alexa 647, magenta; A 3 ), and merged (A 4 ) with nuclear DAPI (blue) in sham, normothermia (NT), and therapeutic hypothermia (TH) mice. Box and whisker plots demonstrate the percent expression of GABA A Rα1 in each group (B 1 ) and the correlations with the number of PV + interneurons (INs) in the CA1 region (B 2 ). In some instances, GABA A Rα1 immunoreactivity was extreme despite blocking methods (C a ) and absent of non-specific staining in negative controls (C b ). Correlations between GABA A Rα1 and hippocampal (Hp) injury scores (D), Hp volume measurements (E), and latency to stage 5 (S5) seizures with flurothyl (F) are shown. Adjustment of the number of PV + INs by their relative expression of GABA A Rα1 affects the correlation with latency to S5 seizures with flurothyl (G), now becoming linear. Boxes are limited by the 25th and 75th percentiles (interquartile range, IQR) and whiskers are limited by the last data point within 1.5 times the IQR from the median (continuous line inside the box), with outliers included. Kruskal–Wallis ANOVA with Dunn-Bonferoni post-hoc testing for pair analysis was applied. *, p < 0.05. In correlation charts, the continuous lines represent the fitted line derived from regression modeling and the discontinuous lines represent the 95% confidence boundries.

Article Snippet: Sections were then exposed to the following primary antibodies: i) chicken anti-parvalbumin (PV, Novus Biological LLC, Centennial, CO; 1:250), ii) rabbit anti-somatostatin (SST, Genetex Inc., Irvine, CA; 1:400), iii) rabbit anti-calretinin (CAL, Abcam PLC, Cambridge, MA; 1:250), or mouse anti-CAL (ProteinTech Inc., Rosemont, IL; 1:250), iv) mouse anti-GABA A α1 subunit (StressMarq Inc., Victoria, British Columbia; 1:400), or rabbit anti-GABA A α1 subunit (Thermo Fisher Scientific Inc., Waltham MA; 1:400), v) mouse anti-GABA B R1 (Abcam PLC; 1:400) and vi) rabbit anti-GABA B R2 (Abcam PLC; 1:400).

Techniques: Whisker Assay, Expressing, Blocking Assay, Staining, Derivative Assay