SIH-391 Search Results


bafa1  (StressMarq)
91
StressMarq bafa1
Effect of OSGIN1 on the autophagic flux. OSGIN1 was either overexpressed by lentiviral vector or suppressed by siRNA in the primary basal cells and then treated with <t>BafA1,</t> a late stage inhibitor of autophagic flux. The changes of autophagy markers MAP1LC3B and SQSTM1 were assessed by western analysis with ACTB as a loading control. (A) Overexpression of OSGIN1 with BafA1 treatment. Fold-changes between lenti-OSGIN1 and lenti-control from 3 independent experiments are shown. Note the consistent upregulation of SQSTM1 and MAP1LC3B-II by lenti-OSGIN1 with or without BafA1 (all fold-changes >1). (B) Knockdown OSGIN1 with BafA1 treatment. Folds changes between si-OSGIN1 and si-control from 3 independent experiments are shown. Note there are no consistent changes.
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Effect of OSGIN1 on the autophagic flux. OSGIN1 was either overexpressed by lentiviral vector or suppressed by siRNA in the primary basal cells and then treated with BafA1, a late stage inhibitor of autophagic flux. The changes of autophagy markers MAP1LC3B and SQSTM1 were assessed by western analysis with ACTB as a loading control. (A) Overexpression of OSGIN1 with BafA1 treatment. Fold-changes between lenti-OSGIN1 and lenti-control from 3 independent experiments are shown. Note the consistent upregulation of SQSTM1 and MAP1LC3B-II by lenti-OSGIN1 with or without BafA1 (all fold-changes >1). (B) Knockdown OSGIN1 with BafA1 treatment. Folds changes between si-OSGIN1 and si-control from 3 independent experiments are shown. Note there are no consistent changes.

Journal: Autophagy

Article Title: Role of OSGIN1 in mediating smoking-induced autophagy in the human airway epithelium

doi: 10.1080/15548627.2017.1301327

Figure Lengend Snippet: Effect of OSGIN1 on the autophagic flux. OSGIN1 was either overexpressed by lentiviral vector or suppressed by siRNA in the primary basal cells and then treated with BafA1, a late stage inhibitor of autophagic flux. The changes of autophagy markers MAP1LC3B and SQSTM1 were assessed by western analysis with ACTB as a loading control. (A) Overexpression of OSGIN1 with BafA1 treatment. Fold-changes between lenti-OSGIN1 and lenti-control from 3 independent experiments are shown. Note the consistent upregulation of SQSTM1 and MAP1LC3B-II by lenti-OSGIN1 with or without BafA1 (all fold-changes >1). (B) Knockdown OSGIN1 with BafA1 treatment. Folds changes between si-OSGIN1 and si-control from 3 independent experiments are shown. Note there are no consistent changes.

Article Snippet: 12,15,17,21 BafA1 (StressMarq, SKC-135) was used (100 nM, 2 h) to block the late-stage autophagic flux.

Techniques: Plasmid Preparation, Western Blot, Over Expression