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  • 99
    Cell Signaling Technology Inc akt
    Suppression of the response to angiotensin II by teneligliptin in vascular smooth muscle cells. (A) Western blot analysis of phosphorylation of <t>ERK</t> and <t>Akt</t> in angiotensin (Ang) II (10 −7 M)-stimulated VSMC in the presence or absence (CTRL) of teneligliptin (DPP-4i, 0, 10, 100 µM) treatment is shown. (B) The ratios of phosphorylated ERK/Akt to total ERK/Akt were quantified. N = 5. * P
    Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/akt/product/Cell Signaling Technology Inc
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    99
    Cell Signaling Technology Inc p38
    Inhibition of <t>p38</t> and ERK attenuates Mn-induced oxidative stress and cell death. ( A,B ) After pre-treatment with LRRK2 inhibitors GSK (1 μM) and MLi-2 (50 nM), p38 inhibitor SB 203580, or ERK inhibitor PD 98059 for 90 min, cells were exposed to Mn (250 μM) for the designated time periods, followed by ROS and MTT assays as described in the Methods section, ( ### , p
    P38, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p38/product/Cell Signaling Technology Inc
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    86
    Promega mek inhibitor u0126
    Inhibition of <t>p38</t> and ERK attenuates Mn-induced oxidative stress and cell death. ( A,B ) After pre-treatment with LRRK2 inhibitors GSK (1 μM) and MLi-2 (50 nM), p38 inhibitor SB 203580, or ERK inhibitor PD 98059 for 90 min, cells were exposed to Mn (250 μM) for the designated time periods, followed by ROS and MTT assays as described in the Methods section, ( ### , p
    Mek Inhibitor U0126, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc phospho erk thr202 tyr204
    Inhibition of <t>p38</t> and ERK attenuates Mn-induced oxidative stress and cell death. ( A,B ) After pre-treatment with LRRK2 inhibitors GSK (1 μM) and MLi-2 (50 nM), p38 inhibitor SB 203580, or ERK inhibitor PD 98059 for 90 min, cells were exposed to Mn (250 μM) for the designated time periods, followed by ROS and MTT assays as described in the Methods section, ( ### , p
    Phospho Erk Thr202 Tyr204, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho erk thr202 tyr204/product/Cell Signaling Technology Inc
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    Image Search Results


    Suppression of the response to angiotensin II by teneligliptin in vascular smooth muscle cells. (A) Western blot analysis of phosphorylation of ERK and Akt in angiotensin (Ang) II (10 −7 M)-stimulated VSMC in the presence or absence (CTRL) of teneligliptin (DPP-4i, 0, 10, 100 µM) treatment is shown. (B) The ratios of phosphorylated ERK/Akt to total ERK/Akt were quantified. N = 5. * P

    Journal: Journal of Atherosclerosis and Thrombosis

    Article Title: Suppression of Abdominal Aortic Aneurysm Formation in Mice by Teneligliptin, a Dipeptidyl Peptidase-4 Inhibitor

    doi: 10.5551/jat.42481

    Figure Lengend Snippet: Suppression of the response to angiotensin II by teneligliptin in vascular smooth muscle cells. (A) Western blot analysis of phosphorylation of ERK and Akt in angiotensin (Ang) II (10 −7 M)-stimulated VSMC in the presence or absence (CTRL) of teneligliptin (DPP-4i, 0, 10, 100 µM) treatment is shown. (B) The ratios of phosphorylated ERK/Akt to total ERK/Akt were quantified. N = 5. * P

    Article Snippet: Antibodies against phosphorylated (p) extracellular signal-regulated kinase (ERK, 9106) and pAKT (9271), and total ERK (9102) and AKT (9272) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

    Techniques: Western Blot

    Inhibition of p38 and ERK attenuates Mn-induced oxidative stress and cell death. ( A,B ) After pre-treatment with LRRK2 inhibitors GSK (1 μM) and MLi-2 (50 nM), p38 inhibitor SB 203580, or ERK inhibitor PD 98059 for 90 min, cells were exposed to Mn (250 μM) for the designated time periods, followed by ROS and MTT assays as described in the Methods section, ( ### , p

    Journal: PLoS ONE

    Article Title: LRRK2 kinase plays a critical role in manganese-induced inflammation and apoptosis in microglia

    doi: 10.1371/journal.pone.0210248

    Figure Lengend Snippet: Inhibition of p38 and ERK attenuates Mn-induced oxidative stress and cell death. ( A,B ) After pre-treatment with LRRK2 inhibitors GSK (1 μM) and MLi-2 (50 nM), p38 inhibitor SB 203580, or ERK inhibitor PD 98059 for 90 min, cells were exposed to Mn (250 μM) for the designated time periods, followed by ROS and MTT assays as described in the Methods section, ( ### , p

    Article Snippet: Antibodies for phospho-p38 (4511S) and p38 (9212) were from Cell Signaling Technology (Danvers, MA).

    Techniques: Inhibition, MTT Assay

    Inhibition of LRRK2 kinase activity attenuates Mn-induced MAPK p38 and ERK, but not JNK in RAW 264.7 and HMC3 cells. ( A,C,E ) After pre-treatment with GSK (1 μM) for 90 min, cells were exposed to Mn (250 μM) for 30 min, followed by total protein extraction and western blot analysis as described in the Methods section. ( B,D,F ) The levels of total and phosphorylated p38 (B), ERK (D) and JNK (F) were quantified. @ , p

    Journal: PLoS ONE

    Article Title: LRRK2 kinase plays a critical role in manganese-induced inflammation and apoptosis in microglia

    doi: 10.1371/journal.pone.0210248

    Figure Lengend Snippet: Inhibition of LRRK2 kinase activity attenuates Mn-induced MAPK p38 and ERK, but not JNK in RAW 264.7 and HMC3 cells. ( A,C,E ) After pre-treatment with GSK (1 μM) for 90 min, cells were exposed to Mn (250 μM) for 30 min, followed by total protein extraction and western blot analysis as described in the Methods section. ( B,D,F ) The levels of total and phosphorylated p38 (B), ERK (D) and JNK (F) were quantified. @ , p

    Article Snippet: Antibodies for phospho-p38 (4511S) and p38 (9212) were from Cell Signaling Technology (Danvers, MA).

    Techniques: Inhibition, Activity Assay, Protein Extraction, Western Blot

    LRRK2 mediates Mn-induced MAPK p38 and ERK, but not JNK signaling in RAW 264.7 cells. ( A,C,E ) After LRRK2 WT and KO cells were exposed to Mn (250 μM) for up to 60 min, the protein was extracted, followed by western blot analysis to detect phosphorylation of signaling proteins as described in the Methods section. ( B,D,F ) The levels of total and phosphorylated MAPK p38 (B), ERK (D) and JNK (F) were quantified. **, p

    Journal: PLoS ONE

    Article Title: LRRK2 kinase plays a critical role in manganese-induced inflammation and apoptosis in microglia

    doi: 10.1371/journal.pone.0210248

    Figure Lengend Snippet: LRRK2 mediates Mn-induced MAPK p38 and ERK, but not JNK signaling in RAW 264.7 cells. ( A,C,E ) After LRRK2 WT and KO cells were exposed to Mn (250 μM) for up to 60 min, the protein was extracted, followed by western blot analysis to detect phosphorylation of signaling proteins as described in the Methods section. ( B,D,F ) The levels of total and phosphorylated MAPK p38 (B), ERK (D) and JNK (F) were quantified. **, p

    Article Snippet: Antibodies for phospho-p38 (4511S) and p38 (9212) were from Cell Signaling Technology (Danvers, MA).

    Techniques: Western Blot