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  • 94
    R&D Systems human trem 2 antibody
    Human Trem 2 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Jackson Laboratory jackson laboratory rrid imsr jax
    Jackson Laboratory Rrid Imsr Jax, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sino Biological trem 2 matched elisa antibody pair set human
    Trem 2 Matched Elisa Antibody Pair Set Human, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher anti erk1 2
    <t>Ras-ERK1/2</t> repressed H3K9 acetylation in SW48 cells via degradation of PCAF. a The mRNA level of PCAF after the indicated transfected was tested. b and c Western blot analysis was performed to measure the expression of PCAF, HDAC2, and H3K9ac following the indicated transfection. Anti-HA antibody was used for testing the exogenous levels of PCAF and HDAC2. d ChIP analysis for testing PCAF levels when different genes were expressed. e 25 μM of MG132 was used to treat cells, after which Western blot was performed to reassess PCAF level. Protein expression of H3K9ac was monitored in the f absence or g presence of MG132. ** P
    Anti Erk1 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti erk1 2/product/Thermo Fisher
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    Image Search Results


    Ras-ERK1/2 repressed H3K9 acetylation in SW48 cells via degradation of PCAF. a The mRNA level of PCAF after the indicated transfected was tested. b and c Western blot analysis was performed to measure the expression of PCAF, HDAC2, and H3K9ac following the indicated transfection. Anti-HA antibody was used for testing the exogenous levels of PCAF and HDAC2. d ChIP analysis for testing PCAF levels when different genes were expressed. e 25 μM of MG132 was used to treat cells, after which Western blot was performed to reassess PCAF level. Protein expression of H3K9ac was monitored in the f absence or g presence of MG132. ** P

    Journal: BMC Cancer

    Article Title: Ras-ERK1/2 signaling contributes to the development of colorectal cancer via regulating H3K9ac

    doi: 10.1186/s12885-018-5199-3

    Figure Lengend Snippet: Ras-ERK1/2 repressed H3K9 acetylation in SW48 cells via degradation of PCAF. a The mRNA level of PCAF after the indicated transfected was tested. b and c Western blot analysis was performed to measure the expression of PCAF, HDAC2, and H3K9ac following the indicated transfection. Anti-HA antibody was used for testing the exogenous levels of PCAF and HDAC2. d ChIP analysis for testing PCAF levels when different genes were expressed. e 25 μM of MG132 was used to treat cells, after which Western blot was performed to reassess PCAF level. Protein expression of H3K9ac was monitored in the f absence or g presence of MG132. ** P

    Article Snippet: Anti-p-ERK1/2 (MA5–15173) and anti-PCAF (MA5–11186) were purchased from Invitrogen; anti-ERK1/2 (ab17942), anti-H3K9ac (ab4441), anti-H3 (ab12079), anti-HA (ab1424), anti-GFP (ab6556), anti-actin (ab8227), anti-His (ab197049), and anti-MDM2 (ab38618) were all purchased from Abcam (Cambridge, MA).

    Techniques: Transfection, Western Blot, Expressing, Chromatin Immunoprecipitation

    Ras-ERK1/2 regulated H3K9ac via MDM2-mediated PCAF degradation. SW48 cells were transfected with pEGFP-K-Ras G12V/T35S , PCAF-HA, MDM2-His (with increasing amount 0.5, 1, and 2 g) and pEGFP-N1. a Exogenous and b endogenous expression of PCAF was measured by Western blot. SW48 cells were transfected either by MDM2-His (2 g) or by MDM2 mutated type (MDM2-MU), then c exogenous and d endogenous expression of PCAF were reassessed. Anti-His and anti-HA antibodies were used for testing the exogenous levels of MDM2 and PCAF, respectively. e MDM2 and H3K9ac expression in cells transfected with pEGFP-K-Ras G12V/T35S or pEGFP-N1. f The protein level of MDM2 after siRNA transfection was tested. g After the indicated transfection, H3K9ac level was tested by Western blot

    Journal: BMC Cancer

    Article Title: Ras-ERK1/2 signaling contributes to the development of colorectal cancer via regulating H3K9ac

    doi: 10.1186/s12885-018-5199-3

    Figure Lengend Snippet: Ras-ERK1/2 regulated H3K9ac via MDM2-mediated PCAF degradation. SW48 cells were transfected with pEGFP-K-Ras G12V/T35S , PCAF-HA, MDM2-His (with increasing amount 0.5, 1, and 2 g) and pEGFP-N1. a Exogenous and b endogenous expression of PCAF was measured by Western blot. SW48 cells were transfected either by MDM2-His (2 g) or by MDM2 mutated type (MDM2-MU), then c exogenous and d endogenous expression of PCAF were reassessed. Anti-His and anti-HA antibodies were used for testing the exogenous levels of MDM2 and PCAF, respectively. e MDM2 and H3K9ac expression in cells transfected with pEGFP-K-Ras G12V/T35S or pEGFP-N1. f The protein level of MDM2 after siRNA transfection was tested. g After the indicated transfection, H3K9ac level was tested by Western blot

    Article Snippet: Anti-p-ERK1/2 (MA5–15173) and anti-PCAF (MA5–11186) were purchased from Invitrogen; anti-ERK1/2 (ab17942), anti-H3K9ac (ab4441), anti-H3 (ab12079), anti-HA (ab1424), anti-GFP (ab6556), anti-actin (ab8227), anti-His (ab197049), and anti-MDM2 (ab38618) were all purchased from Abcam (Cambridge, MA).

    Techniques: Transfection, Expressing, Western Blot

    Ras-ERK1/2 repressed H3K9 acetylation in SW48 cells. SW48 cells were transfected with empty pEGFP vector, pEGFP-K-Ras-WT (wild type) or pEGFP-K-Ras G12V/T35S construct. Protein levels of a p-ERK1/2 and b and c H3K9ac were measured by Western blot analysis. ** P

    Journal: BMC Cancer

    Article Title: Ras-ERK1/2 signaling contributes to the development of colorectal cancer via regulating H3K9ac

    doi: 10.1186/s12885-018-5199-3

    Figure Lengend Snippet: Ras-ERK1/2 repressed H3K9 acetylation in SW48 cells. SW48 cells were transfected with empty pEGFP vector, pEGFP-K-Ras-WT (wild type) or pEGFP-K-Ras G12V/T35S construct. Protein levels of a p-ERK1/2 and b and c H3K9ac were measured by Western blot analysis. ** P

    Article Snippet: Anti-p-ERK1/2 (MA5–15173) and anti-PCAF (MA5–11186) were purchased from Invitrogen; anti-ERK1/2 (ab17942), anti-H3K9ac (ab4441), anti-H3 (ab12079), anti-HA (ab1424), anti-GFP (ab6556), anti-actin (ab8227), anti-His (ab197049), and anti-MDM2 (ab38618) were all purchased from Abcam (Cambridge, MA).

    Techniques: Transfection, Plasmid Preparation, Construct, Western Blot

    Ras-ERK1/2 repressed H3K9 acetylation to promote the growth and migration of SW48 cells. a SW48 cells were transfected with pEGFP-H3, pEGFP-K-Ras G12V/T35S plus pEGFP-H3, or pEGFP-K-Ras G12V/T35S plus pEGFP-H3K9Q (with increasing amount 0.5, 1, and 2 g). a Transfection efficiency was tested by Western blot. b MTT assay was performed to assess cell viability. Subsequently, pEGFP-H3, pEGFP-K-Ras G12V/T35S plus pEGFP-H3, or pEGFP-K-Ras G12V/T35S plus pEGFP-H3K9Q (2 g) was transfected into cell, and c number of colonies and d cell migration were respectively determined by colony formation assay and transwell assay. ** P

    Journal: BMC Cancer

    Article Title: Ras-ERK1/2 signaling contributes to the development of colorectal cancer via regulating H3K9ac

    doi: 10.1186/s12885-018-5199-3

    Figure Lengend Snippet: Ras-ERK1/2 repressed H3K9 acetylation to promote the growth and migration of SW48 cells. a SW48 cells were transfected with pEGFP-H3, pEGFP-K-Ras G12V/T35S plus pEGFP-H3, or pEGFP-K-Ras G12V/T35S plus pEGFP-H3K9Q (with increasing amount 0.5, 1, and 2 g). a Transfection efficiency was tested by Western blot. b MTT assay was performed to assess cell viability. Subsequently, pEGFP-H3, pEGFP-K-Ras G12V/T35S plus pEGFP-H3, or pEGFP-K-Ras G12V/T35S plus pEGFP-H3K9Q (2 g) was transfected into cell, and c number of colonies and d cell migration were respectively determined by colony formation assay and transwell assay. ** P

    Article Snippet: Anti-p-ERK1/2 (MA5–15173) and anti-PCAF (MA5–11186) were purchased from Invitrogen; anti-ERK1/2 (ab17942), anti-H3K9ac (ab4441), anti-H3 (ab12079), anti-HA (ab1424), anti-GFP (ab6556), anti-actin (ab8227), anti-His (ab197049), and anti-MDM2 (ab38618) were all purchased from Abcam (Cambridge, MA).

    Techniques: Migration, Transfection, Western Blot, MTT Assay, Colony Assay, Transwell Assay

    Ras-ERK1/2 repressed H3K9 acetylation to affect the transcription of Ras downstream genes. SW48 cells were transfected with pEGFP-H3, pEGFP-K-Ras G12V/T35S plus pEGFP-H3, or pEGFP-K-Ras G12V/T35S plus pEGFP-H3K9Q. a RT-qPCR was performed to assess the expression levels of these genes. b ChIP was conducted to assess the levels of H3K9ac when different genes were expressed. * P

    Journal: BMC Cancer

    Article Title: Ras-ERK1/2 signaling contributes to the development of colorectal cancer via regulating H3K9ac

    doi: 10.1186/s12885-018-5199-3

    Figure Lengend Snippet: Ras-ERK1/2 repressed H3K9 acetylation to affect the transcription of Ras downstream genes. SW48 cells were transfected with pEGFP-H3, pEGFP-K-Ras G12V/T35S plus pEGFP-H3, or pEGFP-K-Ras G12V/T35S plus pEGFP-H3K9Q. a RT-qPCR was performed to assess the expression levels of these genes. b ChIP was conducted to assess the levels of H3K9ac when different genes were expressed. * P

    Article Snippet: Anti-p-ERK1/2 (MA5–15173) and anti-PCAF (MA5–11186) were purchased from Invitrogen; anti-ERK1/2 (ab17942), anti-H3K9ac (ab4441), anti-H3 (ab12079), anti-HA (ab1424), anti-GFP (ab6556), anti-actin (ab8227), anti-His (ab197049), and anti-MDM2 (ab38618) were all purchased from Abcam (Cambridge, MA).

    Techniques: Transfection, Quantitative RT-PCR, Expressing, Chromatin Immunoprecipitation