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    New England Biolabs snap cell 505 star
    HDAC1 associates with components of the prefoldin-mediated CCT folding pathway. ( A used to model possible HDAC1 processing by the prefoldin/CCT pathway in the nucleus. ( B . ( C ) VBP1 colocalises with HDAC1 in the nucleus. Flp-In™-293 host cells stably expressing Halo-HDAC1 were transiently transfected with <t>SNAP-FLAG-VBP1</t> and proteins imaged using HaloTag® TMRDirect™ ligand (Halo-HDAC1; red), SNAP-Cell® 505-Star ligand (SNAP-FLAG-VBP1; green), and Hoechst dye (nuclei; blue). ( D network showing established TCP1 (CCT complex) interacting proteins was generated using the following settings: minimum required interaction score - medium confidence (0.4); maximum number of interactors - 50; active interaction source – Experiments; clustering method – kmeans (default settings). Clusters are indicated by node color.
    Snap Cell 505 Star, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap cell 505 star/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
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    snap cell 505 star - by Bioz Stars, 2021-05
    95/100 stars
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    HDAC1 associates with components of the prefoldin-mediated CCT folding pathway. ( A used to model possible HDAC1 processing by the prefoldin/CCT pathway in the nucleus. ( B . ( C ) VBP1 colocalises with HDAC1 in the nucleus. Flp-In™-293 host cells stably expressing Halo-HDAC1 were transiently transfected with SNAP-FLAG-VBP1 and proteins imaged using HaloTag® TMRDirect™ ligand (Halo-HDAC1; red), SNAP-Cell® 505-Star ligand (SNAP-FLAG-VBP1; green), and Hoechst dye (nuclei; blue). ( D network showing established TCP1 (CCT complex) interacting proteins was generated using the following settings: minimum required interaction score - medium confidence (0.4); maximum number of interactors - 50; active interaction source – Experiments; clustering method – kmeans (default settings). Clusters are indicated by node color.

    Journal: Scientific Reports

    Article Title: Differential HDAC1/2 network analysis reveals a role for prefoldin/CCT in HDAC1/2 complex assembly

    doi: 10.1038/s41598-018-32009-w

    Figure Lengend Snippet: HDAC1 associates with components of the prefoldin-mediated CCT folding pathway. ( A used to model possible HDAC1 processing by the prefoldin/CCT pathway in the nucleus. ( B . ( C ) VBP1 colocalises with HDAC1 in the nucleus. Flp-In™-293 host cells stably expressing Halo-HDAC1 were transiently transfected with SNAP-FLAG-VBP1 and proteins imaged using HaloTag® TMRDirect™ ligand (Halo-HDAC1; red), SNAP-Cell® 505-Star ligand (SNAP-FLAG-VBP1; green), and Hoechst dye (nuclei; blue). ( D network showing established TCP1 (CCT complex) interacting proteins was generated using the following settings: minimum required interaction score - medium confidence (0.4); maximum number of interactors - 50; active interaction source – Experiments; clustering method – kmeans (default settings). Clusters are indicated by node color.

    Article Snippet: SNAP-tagged VBP1 was similarly imaged using SNAP-Cell® 505-Star ligand (NEB) according to the manufacturer’s instructions.

    Techniques: Stable Transfection, Expressing, Transfection, Generated