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snap cell 505 star  (New England Biolabs)
94
New England Biolabs snap cell 505 star
Examination of the functionality of SNAP was determined by using a fluorescent label modified with a BG group (SNAP-Cell ® 505-Star). ( a ) Schematic of surface-display system and ( b ) fluorescent label addition. ( c ) Histogram and ( d ) bar graph from flow cytometry analysis showing the shift in fluorescence of cells labelled after growth with and without inducer. Tests were performed in triplicate with 10 000 events per sample (* P < 0.0001, n = 3). ( e ) Dose response curve of the labelled surface-displayed SNAP.
Snap Cell 505 Star, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Examination of the functionality of SNAP was determined by using a fluorescent label modified with a BG group (SNAP-Cell ® 505-Star). ( a ) Schematic of surface-display system and ( b ) fluorescent label addition. ( c ) Histogram and ( d ) bar graph from flow cytometry analysis showing the shift in fluorescence of cells labelled after growth with and without inducer. Tests were performed in triplicate with 10 000 events per sample (* P < 0.0001, n = 3). ( e ) Dose response curve of the labelled surface-displayed SNAP.

Journal: Synthetic Biology

Article Title: Engineering a living biomaterial via bacterial surface capture of environmental molecules

doi: 10.1093/synbio/ysy017

Figure Lengend Snippet: Examination of the functionality of SNAP was determined by using a fluorescent label modified with a BG group (SNAP-Cell ® 505-Star). ( a ) Schematic of surface-display system and ( b ) fluorescent label addition. ( c ) Histogram and ( d ) bar graph from flow cytometry analysis showing the shift in fluorescence of cells labelled after growth with and without inducer. Tests were performed in triplicate with 10 000 events per sample (* P < 0.0001, n = 3). ( e ) Dose response curve of the labelled surface-displayed SNAP.

Article Snippet: SNAP-Cell ® 505-Star is a fluorescent label purchased from New England Biolabs, Inc., with an excitation peak at 504 nm and an emission peak at 532 nm.

Techniques: Modification, Flow Cytometry, Fluorescence