S-133 Search Results


human mouse rat phospho creb s133 duoset ic elisa kit  (R&D Systems)
93
R&D Systems human mouse rat phospho creb s133 duoset ic elisa kit
Human Mouse Rat Phospho Creb S133 Duoset Ic Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3165009a vendor p rb  (fluidigm)
94
fluidigm 3165009a vendor p rb
3165009a Vendor P Rb, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3176005a pakt 152sm  (fluidigm)
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fluidigm 3176005a pakt 152sm
3176005a Pakt 152sm, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti p creb  (Boster Bio)
92
Boster Bio rabbit anti p creb
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3165034d  (fluidigm)
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fluidigm 3165034d
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anti phospho creb1 ser133 antibody  (Biorbyt)
93
Biorbyt anti phospho creb1 ser133 antibody
Compound 145 activates <t>cAMP/PKA/CREB</t> dependent pathway in TGF-β 1 -induced MRC-5 cells. ( A ) Lung fibroblasts were cultured for 24 h and then serum-deprived for 2 h. The cAMP level was measured after 30 min of incubation with the studied compounds (10 µM) and TGF-β 1 (5 ng/mL). Each bar represents the mean (± S.E.M.) The cAMP concentration (fmol/mL/mg of protein; n = 4). ( B ) Protein kinase A activity in MRC-5 was determined after 30 min of incubation with the studied compounds (10 µM), and 30 min of incubation with TGF-β 1 ( n = 4). ( C ) Representative immunoblot and densitometry analysis of p-CREB in MRC-5 ( n = 4). The ROD signal was normalized to the GAPDH control level. Each bar represents the mean value (± S.E.M.). ( D ) Immunofluorescence staining for p-CREB protein: MRC-5 were fixed, permeabilized, blocked, and incubated with anti-p-CREB antibody followed by an Alexa Fluor 546 conjugated antibody, nuclei were stained with Hoechst 33342 dye. Microphotographs were taken using a Leica DMi8 microscope, 40× objective, bar = 50 µm. ( E ) Cells exhibiting co-localization of p-CREB and nuclear signal were counted in 20 randomly selected fields of view and expressed as a percentage fraction in the entire MRC-5 population. The results were considered statistically significant at the p level of 0.05, against control (*).
Anti Phospho Creb1 Ser133 Antibody, supplied by Biorbyt, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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duoset elisa  (R&D Systems)
92
R&D Systems duoset elisa
Compound 145 activates <t>cAMP/PKA/CREB</t> dependent pathway in TGF-β 1 -induced MRC-5 cells. ( A ) Lung fibroblasts were cultured for 24 h and then serum-deprived for 2 h. The cAMP level was measured after 30 min of incubation with the studied compounds (10 µM) and TGF-β 1 (5 ng/mL). Each bar represents the mean (± S.E.M.) The cAMP concentration (fmol/mL/mg of protein; n = 4). ( B ) Protein kinase A activity in MRC-5 was determined after 30 min of incubation with the studied compounds (10 µM), and 30 min of incubation with TGF-β 1 ( n = 4). ( C ) Representative immunoblot and densitometry analysis of p-CREB in MRC-5 ( n = 4). The ROD signal was normalized to the GAPDH control level. Each bar represents the mean value (± S.E.M.). ( D ) Immunofluorescence staining for p-CREB protein: MRC-5 were fixed, permeabilized, blocked, and incubated with anti-p-CREB antibody followed by an Alexa Fluor 546 conjugated antibody, nuclei were stained with Hoechst 33342 dye. Microphotographs were taken using a Leica DMi8 microscope, 40× objective, bar = 50 µm. ( E ) Cells exhibiting co-localization of p-CREB and nuclear signal were counted in 20 randomly selected fields of view and expressed as a percentage fraction in the entire MRC-5 population. The results were considered statistically significant at the p level of 0.05, against control (*).
Duoset Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s133  (Biorbyt)
92
Biorbyt s133
Compound 145 activates <t>cAMP/PKA/CREB</t> dependent pathway in TGF-β 1 -induced MRC-5 cells. ( A ) Lung fibroblasts were cultured for 24 h and then serum-deprived for 2 h. The cAMP level was measured after 30 min of incubation with the studied compounds (10 µM) and TGF-β 1 (5 ng/mL). Each bar represents the mean (± S.E.M.) The cAMP concentration (fmol/mL/mg of protein; n = 4). ( B ) Protein kinase A activity in MRC-5 was determined after 30 min of incubation with the studied compounds (10 µM), and 30 min of incubation with TGF-β 1 ( n = 4). ( C ) Representative immunoblot and densitometry analysis of p-CREB in MRC-5 ( n = 4). The ROD signal was normalized to the GAPDH control level. Each bar represents the mean value (± S.E.M.). ( D ) Immunofluorescence staining for p-CREB protein: MRC-5 were fixed, permeabilized, blocked, and incubated with anti-p-CREB antibody followed by an Alexa Fluor 546 conjugated antibody, nuclei were stained with Hoechst 33342 dye. Microphotographs were taken using a Leica DMi8 microscope, 40× objective, bar = 50 µm. ( E ) Cells exhibiting co-localization of p-CREB and nuclear signal were counted in 20 randomly selected fields of view and expressed as a percentage fraction in the entire MRC-5 population. The results were considered statistically significant at the p level of 0.05, against control (*).
S133, supplied by Biorbyt, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-phospho-creb (s133)  (ABclonal Biotechnology)
90
ABclonal Biotechnology anti-phospho-creb (s133)
Compound 145 activates <t>cAMP/PKA/CREB</t> dependent pathway in TGF-β 1 -induced MRC-5 cells. ( A ) Lung fibroblasts were cultured for 24 h and then serum-deprived for 2 h. The cAMP level was measured after 30 min of incubation with the studied compounds (10 µM) and TGF-β 1 (5 ng/mL). Each bar represents the mean (± S.E.M.) The cAMP concentration (fmol/mL/mg of protein; n = 4). ( B ) Protein kinase A activity in MRC-5 was determined after 30 min of incubation with the studied compounds (10 µM), and 30 min of incubation with TGF-β 1 ( n = 4). ( C ) Representative immunoblot and densitometry analysis of p-CREB in MRC-5 ( n = 4). The ROD signal was normalized to the GAPDH control level. Each bar represents the mean value (± S.E.M.). ( D ) Immunofluorescence staining for p-CREB protein: MRC-5 were fixed, permeabilized, blocked, and incubated with anti-p-CREB antibody followed by an Alexa Fluor 546 conjugated antibody, nuclei were stained with Hoechst 33342 dye. Microphotographs were taken using a Leica DMi8 microscope, 40× objective, bar = 50 µm. ( E ) Cells exhibiting co-localization of p-CREB and nuclear signal were counted in 20 randomly selected fields of view and expressed as a percentage fraction in the entire MRC-5 population. The results were considered statistically significant at the p level of 0.05, against control (*).
Anti Phospho Creb (S133), supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s133  (Toray Industries)
90
Toray Industries s133
Compound 145 activates <t>cAMP/PKA/CREB</t> dependent pathway in TGF-β 1 -induced MRC-5 cells. ( A ) Lung fibroblasts were cultured for 24 h and then serum-deprived for 2 h. The cAMP level was measured after 30 min of incubation with the studied compounds (10 µM) and TGF-β 1 (5 ng/mL). Each bar represents the mean (± S.E.M.) The cAMP concentration (fmol/mL/mg of protein; n = 4). ( B ) Protein kinase A activity in MRC-5 was determined after 30 min of incubation with the studied compounds (10 µM), and 30 min of incubation with TGF-β 1 ( n = 4). ( C ) Representative immunoblot and densitometry analysis of p-CREB in MRC-5 ( n = 4). The ROD signal was normalized to the GAPDH control level. Each bar represents the mean value (± S.E.M.). ( D ) Immunofluorescence staining for p-CREB protein: MRC-5 were fixed, permeabilized, blocked, and incubated with anti-p-CREB antibody followed by an Alexa Fluor 546 conjugated antibody, nuclei were stained with Hoechst 33342 dye. Microphotographs were taken using a Leica DMi8 microscope, 40× objective, bar = 50 µm. ( E ) Cells exhibiting co-localization of p-CREB and nuclear signal were counted in 20 randomly selected fields of view and expressed as a percentage fraction in the entire MRC-5 population. The results were considered statistically significant at the p level of 0.05, against control (*).
S133, supplied by Toray Industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-phosphocreb1 (s133)  (Abmart Inc)
90
Abmart Inc anti-phosphocreb1 (s133)
Compound 145 activates <t>cAMP/PKA/CREB</t> dependent pathway in TGF-β 1 -induced MRC-5 cells. ( A ) Lung fibroblasts were cultured for 24 h and then serum-deprived for 2 h. The cAMP level was measured after 30 min of incubation with the studied compounds (10 µM) and TGF-β 1 (5 ng/mL). Each bar represents the mean (± S.E.M.) The cAMP concentration (fmol/mL/mg of protein; n = 4). ( B ) Protein kinase A activity in MRC-5 was determined after 30 min of incubation with the studied compounds (10 µM), and 30 min of incubation with TGF-β 1 ( n = 4). ( C ) Representative immunoblot and densitometry analysis of p-CREB in MRC-5 ( n = 4). The ROD signal was normalized to the GAPDH control level. Each bar represents the mean value (± S.E.M.). ( D ) Immunofluorescence staining for p-CREB protein: MRC-5 were fixed, permeabilized, blocked, and incubated with anti-p-CREB antibody followed by an Alexa Fluor 546 conjugated antibody, nuclei were stained with Hoechst 33342 dye. Microphotographs were taken using a Leica DMi8 microscope, 40× objective, bar = 50 µm. ( E ) Cells exhibiting co-localization of p-CREB and nuclear signal were counted in 20 randomly selected fields of view and expressed as a percentage fraction in the entire MRC-5 population. The results were considered statistically significant at the p level of 0.05, against control (*).
Anti Phosphocreb1 (S133), supplied by Abmart Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dmw 2010; 135: s133–s192  (Georg Thieme Verlag KG)
90
Georg Thieme Verlag KG dmw 2010; 135: s133–s192
Compound 145 activates <t>cAMP/PKA/CREB</t> dependent pathway in TGF-β 1 -induced MRC-5 cells. ( A ) Lung fibroblasts were cultured for 24 h and then serum-deprived for 2 h. The cAMP level was measured after 30 min of incubation with the studied compounds (10 µM) and TGF-β 1 (5 ng/mL). Each bar represents the mean (± S.E.M.) The cAMP concentration (fmol/mL/mg of protein; n = 4). ( B ) Protein kinase A activity in MRC-5 was determined after 30 min of incubation with the studied compounds (10 µM), and 30 min of incubation with TGF-β 1 ( n = 4). ( C ) Representative immunoblot and densitometry analysis of p-CREB in MRC-5 ( n = 4). The ROD signal was normalized to the GAPDH control level. Each bar represents the mean value (± S.E.M.). ( D ) Immunofluorescence staining for p-CREB protein: MRC-5 were fixed, permeabilized, blocked, and incubated with anti-p-CREB antibody followed by an Alexa Fluor 546 conjugated antibody, nuclei were stained with Hoechst 33342 dye. Microphotographs were taken using a Leica DMi8 microscope, 40× objective, bar = 50 µm. ( E ) Cells exhibiting co-localization of p-CREB and nuclear signal were counted in 20 randomly selected fields of view and expressed as a percentage fraction in the entire MRC-5 population. The results were considered statistically significant at the p level of 0.05, against control (*).
Dmw 2010; 135: S133–S192, supplied by Georg Thieme Verlag KG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Compound 145 activates cAMP/PKA/CREB dependent pathway in TGF-β 1 -induced MRC-5 cells. ( A ) Lung fibroblasts were cultured for 24 h and then serum-deprived for 2 h. The cAMP level was measured after 30 min of incubation with the studied compounds (10 µM) and TGF-β 1 (5 ng/mL). Each bar represents the mean (± S.E.M.) The cAMP concentration (fmol/mL/mg of protein; n = 4). ( B ) Protein kinase A activity in MRC-5 was determined after 30 min of incubation with the studied compounds (10 µM), and 30 min of incubation with TGF-β 1 ( n = 4). ( C ) Representative immunoblot and densitometry analysis of p-CREB in MRC-5 ( n = 4). The ROD signal was normalized to the GAPDH control level. Each bar represents the mean value (± S.E.M.). ( D ) Immunofluorescence staining for p-CREB protein: MRC-5 were fixed, permeabilized, blocked, and incubated with anti-p-CREB antibody followed by an Alexa Fluor 546 conjugated antibody, nuclei were stained with Hoechst 33342 dye. Microphotographs were taken using a Leica DMi8 microscope, 40× objective, bar = 50 µm. ( E ) Cells exhibiting co-localization of p-CREB and nuclear signal were counted in 20 randomly selected fields of view and expressed as a percentage fraction in the entire MRC-5 population. The results were considered statistically significant at the p level of 0.05, against control (*).

Journal: International Journal of Molecular Sciences

Article Title: A Novel, Pan-PDE Inhibitor Exerts Anti-Fibrotic Effects in Human Lung Fibroblasts via Inhibition of TGF-β Signaling and Activation of cAMP/PKA Signaling

doi: 10.3390/ijms21114008

Figure Lengend Snippet: Compound 145 activates cAMP/PKA/CREB dependent pathway in TGF-β 1 -induced MRC-5 cells. ( A ) Lung fibroblasts were cultured for 24 h and then serum-deprived for 2 h. The cAMP level was measured after 30 min of incubation with the studied compounds (10 µM) and TGF-β 1 (5 ng/mL). Each bar represents the mean (± S.E.M.) The cAMP concentration (fmol/mL/mg of protein; n = 4). ( B ) Protein kinase A activity in MRC-5 was determined after 30 min of incubation with the studied compounds (10 µM), and 30 min of incubation with TGF-β 1 ( n = 4). ( C ) Representative immunoblot and densitometry analysis of p-CREB in MRC-5 ( n = 4). The ROD signal was normalized to the GAPDH control level. Each bar represents the mean value (± S.E.M.). ( D ) Immunofluorescence staining for p-CREB protein: MRC-5 were fixed, permeabilized, blocked, and incubated with anti-p-CREB antibody followed by an Alexa Fluor 546 conjugated antibody, nuclei were stained with Hoechst 33342 dye. Microphotographs were taken using a Leica DMi8 microscope, 40× objective, bar = 50 µm. ( E ) Cells exhibiting co-localization of p-CREB and nuclear signal were counted in 20 randomly selected fields of view and expressed as a percentage fraction in the entire MRC-5 population. The results were considered statistically significant at the p level of 0.05, against control (*).

Article Snippet: After incubation in blocking solution the following primary antibodies: rabbit polyclonal, anti-phospho-Smad-2 (Ser465, Ser467) antibody (#44-244G, Thermo Fisher Scientific, Waltham, MA, USA); rabbit polyclonal, anti-phospho-CREB1 (Ser133) antibody (#orb213775, Biorbyt, Cambridge, United Kingdom); mouse monoclonal, anti-α-SMA antibody (#MA5-11547, Thermo Fisher Scientific, Waltham, MA, USA) and secondary antibodies: goat anti-rabbit Alexa Fluor 546 conjugated antibody and goat anti-mouse Alexa Fluor 488 conjugated antibody (#A-11010 and #A-11001, respectively, Invitrogen, Carlsbad, CA, USA) were used.

Techniques: Cell Culture, Incubation, Concentration Assay, Activity Assay, Western Blot, Control, Immunofluorescence, Staining, Microscopy

Compound 145 interaction with TGF-β 1 -induced MRC-5 cells and possible targets related to its anti-fibrotic effects. Abbreviations: ATP—adenosine triphosphate; 5′-AMP—5′-adenosine monophosphate; CRE—CREB response element; SBE—Smad binding element, SARA—Smad anchor for receptor activation

Journal: International Journal of Molecular Sciences

Article Title: A Novel, Pan-PDE Inhibitor Exerts Anti-Fibrotic Effects in Human Lung Fibroblasts via Inhibition of TGF-β Signaling and Activation of cAMP/PKA Signaling

doi: 10.3390/ijms21114008

Figure Lengend Snippet: Compound 145 interaction with TGF-β 1 -induced MRC-5 cells and possible targets related to its anti-fibrotic effects. Abbreviations: ATP—adenosine triphosphate; 5′-AMP—5′-adenosine monophosphate; CRE—CREB response element; SBE—Smad binding element, SARA—Smad anchor for receptor activation

Article Snippet: After incubation in blocking solution the following primary antibodies: rabbit polyclonal, anti-phospho-Smad-2 (Ser465, Ser467) antibody (#44-244G, Thermo Fisher Scientific, Waltham, MA, USA); rabbit polyclonal, anti-phospho-CREB1 (Ser133) antibody (#orb213775, Biorbyt, Cambridge, United Kingdom); mouse monoclonal, anti-α-SMA antibody (#MA5-11547, Thermo Fisher Scientific, Waltham, MA, USA) and secondary antibodies: goat anti-rabbit Alexa Fluor 546 conjugated antibody and goat anti-mouse Alexa Fluor 488 conjugated antibody (#A-11010 and #A-11001, respectively, Invitrogen, Carlsbad, CA, USA) were used.

Techniques: Binding Assay, Activation Assay