RL-1002-25 Search Results


rhodamine labelled concanavalin a rhodamine cona  (Vector Laboratories)
93
Vector Laboratories rhodamine labelled concanavalin a rhodamine cona
Rhodamine Labelled Concanavalin A Rhodamine Cona, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/RL-1002-25/Rhodamine+labeled+Concanavalin+A+(Con+A)/pm27915303-54-11-15
Average 93 stars, based on 1 article reviews
rhodamine labelled concanavalin a rhodamine cona - by Bioz Stars, 2026-11
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concanavalin a  (Bio-Techne corporation)
93
Bio-Techne corporation concanavalin a
Effect of CCL8 on immune cells and MSCs. ( A ) T cells, B cells, and NK cells (1 × 10 5 cells/well) were added to the upper wells of transwell plates. CCL8 was added to the lower wells at 30 or 100 ng/ml. The number of cells that migrated to the lower wells over 2 h was counted using a flow cytometer. ( B ) MSCs (2 × 10 4 cells/well) were added to the upper wells of transwell plates. CCL8 was added to the lower wells at 30 or 100 ng/ml. The number of migrating MSCs was counted as described in the “ ”. ( C ) T cells isolated from the spleen of MRL. Fas lpr mice were activated with <t>concanavalin</t> <t>A</t> (Con A, 1 μg/ml) in the presence or absence of recombinant CCL8 for 72 h. IFN-γ level in the medium was determined by ELISA. ( D ) MSCs were pretreated with recombinant CCL8 at 30–300 ng/ml for 24 h. T cells (1 × 10 5 cells/well) were co-cultured with MSCs (1 × 10 4 cells/well) in the presence of anti-CD3 and anti-CD28 antibodies (1 μg/ml each) for 72 h. IFN-γ level in the medium was determined by ELISA. ( E ) MSCs were treated with recombinant CCL8 (300 ng/ml). The levels of mRNAs of the indicated soluble factors were determined by RT-qPCR. ( F ) MSCs were pretreated with recombinant CCL8 (300 ng/ml; CCL8-MSCs). CCL8-MSCs were transfected with negative-control (neg), IL-10, IDO, TGF-β1, or iNOS siRNA. T cells (1 × 10 5 cells/well) were co-cultured with MSCs (0.1 × 10 5 cells/well) for 72 h. Anti-CD3 and anti-CD28 antibodies (1 μg/ml each) were added to activate T cells. IFN-γ level in the medium was determined by ELISA. RQ, Relative quantitation. UN, Untreated. n = 3 in all panels. * p < 0.01.
Concanavalin A, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/RL-1002-25/Concanavalin+A/pmc10421856-295-11-35
Average 93 stars, based on 1 article reviews
concanavalin a - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier

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Effect of CCL8 on immune cells and MSCs. ( A ) T cells, B cells, and NK cells (1 × 10 5 cells/well) were added to the upper wells of transwell plates. CCL8 was added to the lower wells at 30 or 100 ng/ml. The number of cells that migrated to the lower wells over 2 h was counted using a flow cytometer. ( B ) MSCs (2 × 10 4 cells/well) were added to the upper wells of transwell plates. CCL8 was added to the lower wells at 30 or 100 ng/ml. The number of migrating MSCs was counted as described in the “ ”. ( C ) T cells isolated from the spleen of MRL. Fas lpr mice were activated with concanavalin A (Con A, 1 μg/ml) in the presence or absence of recombinant CCL8 for 72 h. IFN-γ level in the medium was determined by ELISA. ( D ) MSCs were pretreated with recombinant CCL8 at 30–300 ng/ml for 24 h. T cells (1 × 10 5 cells/well) were co-cultured with MSCs (1 × 10 4 cells/well) in the presence of anti-CD3 and anti-CD28 antibodies (1 μg/ml each) for 72 h. IFN-γ level in the medium was determined by ELISA. ( E ) MSCs were treated with recombinant CCL8 (300 ng/ml). The levels of mRNAs of the indicated soluble factors were determined by RT-qPCR. ( F ) MSCs were pretreated with recombinant CCL8 (300 ng/ml; CCL8-MSCs). CCL8-MSCs were transfected with negative-control (neg), IL-10, IDO, TGF-β1, or iNOS siRNA. T cells (1 × 10 5 cells/well) were co-cultured with MSCs (0.1 × 10 5 cells/well) for 72 h. Anti-CD3 and anti-CD28 antibodies (1 μg/ml each) were added to activate T cells. IFN-γ level in the medium was determined by ELISA. RQ, Relative quantitation. UN, Untreated. n = 3 in all panels. * p < 0.01.

Journal: Scientific Reports

Article Title: Mesenchymal stem cells enhance CCL8 expression by podocytes in lupus-prone MRL. Fas lpr mice

doi: 10.1038/s41598-023-40346-8

Figure Lengend Snippet: Effect of CCL8 on immune cells and MSCs. ( A ) T cells, B cells, and NK cells (1 × 10 5 cells/well) were added to the upper wells of transwell plates. CCL8 was added to the lower wells at 30 or 100 ng/ml. The number of cells that migrated to the lower wells over 2 h was counted using a flow cytometer. ( B ) MSCs (2 × 10 4 cells/well) were added to the upper wells of transwell plates. CCL8 was added to the lower wells at 30 or 100 ng/ml. The number of migrating MSCs was counted as described in the “ ”. ( C ) T cells isolated from the spleen of MRL. Fas lpr mice were activated with concanavalin A (Con A, 1 μg/ml) in the presence or absence of recombinant CCL8 for 72 h. IFN-γ level in the medium was determined by ELISA. ( D ) MSCs were pretreated with recombinant CCL8 at 30–300 ng/ml for 24 h. T cells (1 × 10 5 cells/well) were co-cultured with MSCs (1 × 10 4 cells/well) in the presence of anti-CD3 and anti-CD28 antibodies (1 μg/ml each) for 72 h. IFN-γ level in the medium was determined by ELISA. ( E ) MSCs were treated with recombinant CCL8 (300 ng/ml). The levels of mRNAs of the indicated soluble factors were determined by RT-qPCR. ( F ) MSCs were pretreated with recombinant CCL8 (300 ng/ml; CCL8-MSCs). CCL8-MSCs were transfected with negative-control (neg), IL-10, IDO, TGF-β1, or iNOS siRNA. T cells (1 × 10 5 cells/well) were co-cultured with MSCs (0.1 × 10 5 cells/well) for 72 h. Anti-CD3 and anti-CD28 antibodies (1 μg/ml each) were added to activate T cells. IFN-γ level in the medium was determined by ELISA. RQ, Relative quantitation. UN, Untreated. n = 3 in all panels. * p < 0.01.

Article Snippet: Purified T cells (1 × 10 5 cells/well) were activated with concanavalin A (Con A, 1 μg/ml) or CD3/CD28 antibodies (1 μg/ml each) for 72 h. IFN-γ level in the medium was determined by ELISA (Biotechne) .

Techniques: Flow Cytometry, Isolation, Recombinant, Enzyme-linked Immunosorbent Assay, Cell Culture, Quantitative RT-PCR, Transfection, Negative Control, Quantitation Assay

Effect of CCL5 on immune cells and MSCs. ( A ) Podocytes (1 × 10 5 cells/well) were loaded into the lower wells of transwell plates. MSCs (1 × 10 4 cells/well) were added to the upper wells to prevent contact or to the lower wells to allow contact with podocytes. After incubation for 24 h, CCL5 level in the medium was determined by ELISA. ( B ) The levels of mRNAs of the indicated soluble factors in MSCs were determined by RT-qPCR. (C, D) MSCs were transfected with negative-control, TGF-β, IDO, or iNOS siRNAs. MSCs (1 × 10 4 cells/well) were co-cultured with podocytes (1 × 10 5 cells/well). After incubation for 24 h, ( C ) expression of CCL5 by podocytes was determined by RT-PCR and ( D ) CCL5 level in the medium was determined by ELISA. ( E ) Podocytes (1 × 10 5 cells/well) were treated with L-kynurenine or recombinant TGF-β1. After incubation for 24 h, CCL5 level in the medium was determined by ELISA. ( F ) T cells, B cells, or NK cells (1 × 10 5 cells/well) were added to the upper wells of transwell plates. Recombinant CCL5 was added to the lower wells at 30 or 100 ng/ml. The number of immune cells that had migrated to the lower wells over 2 h was counted using a flow cytometer. ( G ) MSCs (2 × 10 4 cells/well) were added to the upper wells of transwell plates. Recombinant CCL5 was added to the lower wells at 30 or 100 ng/ml. The number of migrating MSCs was counted as described in the “ ”. ( H ) T cells isolated from the spleen of MRL. Fas lpr mice were activated with concanavalin A (Con A, 1 μg/ml) in the presence or absence of recombinant CCL5 for 72 h. IFN-γlevel in the medium was determined by ELISA. ( I ) Recombinant CCL5 was added to the culture of MSCs for 24 h. The levels of mRNAs of the indicated soluble factors were determined by RT-qPCR. TNF-α was used at 50 ng/ml in all experiments. RQ, Relative quantitation. UN, Untreated. n = 3 in all panels. * p < 0.01.

Journal: Scientific Reports

Article Title: Mesenchymal stem cells enhance CCL8 expression by podocytes in lupus-prone MRL. Fas lpr mice

doi: 10.1038/s41598-023-40346-8

Figure Lengend Snippet: Effect of CCL5 on immune cells and MSCs. ( A ) Podocytes (1 × 10 5 cells/well) were loaded into the lower wells of transwell plates. MSCs (1 × 10 4 cells/well) were added to the upper wells to prevent contact or to the lower wells to allow contact with podocytes. After incubation for 24 h, CCL5 level in the medium was determined by ELISA. ( B ) The levels of mRNAs of the indicated soluble factors in MSCs were determined by RT-qPCR. (C, D) MSCs were transfected with negative-control, TGF-β, IDO, or iNOS siRNAs. MSCs (1 × 10 4 cells/well) were co-cultured with podocytes (1 × 10 5 cells/well). After incubation for 24 h, ( C ) expression of CCL5 by podocytes was determined by RT-PCR and ( D ) CCL5 level in the medium was determined by ELISA. ( E ) Podocytes (1 × 10 5 cells/well) were treated with L-kynurenine or recombinant TGF-β1. After incubation for 24 h, CCL5 level in the medium was determined by ELISA. ( F ) T cells, B cells, or NK cells (1 × 10 5 cells/well) were added to the upper wells of transwell plates. Recombinant CCL5 was added to the lower wells at 30 or 100 ng/ml. The number of immune cells that had migrated to the lower wells over 2 h was counted using a flow cytometer. ( G ) MSCs (2 × 10 4 cells/well) were added to the upper wells of transwell plates. Recombinant CCL5 was added to the lower wells at 30 or 100 ng/ml. The number of migrating MSCs was counted as described in the “ ”. ( H ) T cells isolated from the spleen of MRL. Fas lpr mice were activated with concanavalin A (Con A, 1 μg/ml) in the presence or absence of recombinant CCL5 for 72 h. IFN-γlevel in the medium was determined by ELISA. ( I ) Recombinant CCL5 was added to the culture of MSCs for 24 h. The levels of mRNAs of the indicated soluble factors were determined by RT-qPCR. TNF-α was used at 50 ng/ml in all experiments. RQ, Relative quantitation. UN, Untreated. n = 3 in all panels. * p < 0.01.

Article Snippet: Purified T cells (1 × 10 5 cells/well) were activated with concanavalin A (Con A, 1 μg/ml) or CD3/CD28 antibodies (1 μg/ml each) for 72 h. IFN-γ level in the medium was determined by ELISA (Biotechne) .

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Transfection, Negative Control, Cell Culture, Expressing, Reverse Transcription Polymerase Chain Reaction, Recombinant, Flow Cytometry, Isolation, Quantitation Assay