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pd l1 r30949 antibody  (NSJ Bioreagents)
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NSJ Bioreagents pd l1 r30949 antibody
Gallic acid (GA) reduces the programmed death-ligand 1 <t>(PD-L1)</t> expression in non-small-cell lung cancer (NSCLC) cells. ( A ) The expression levels of PD-L1 protein in A549 and H292 cells were detected after GA treatment in concentrations indicated for 48 hours. ( B ) The relative expression levels of PD-L1 protein were determined by densitometry and normalized to β-actin. Data are representative of three independent experiments. *** p < 0.001 ( t- test). ( C ) The expression levels of PD-L1 mRNA in A549 and H292 cells were detected after GA treatment in concentrations indicated for 48 hours. The relative expression levels of PD-L1 mRNA were determined by real time qPCR and normalized to GAPDH mRNA. Data are representative of three independent experiments. *** p < 0.001 ( t- test).
Pd L1 R30949 Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pd l1 r30949 antibody - by Bioz Stars, 2026-02
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Gallic acid (GA) reduces the programmed death-ligand 1 (PD-L1) expression in non-small-cell lung cancer (NSCLC) cells. ( A ) The expression levels of PD-L1 protein in A549 and H292 cells were detected after GA treatment in concentrations indicated for 48 hours. ( B ) The relative expression levels of PD-L1 protein were determined by densitometry and normalized to β-actin. Data are representative of three independent experiments. *** p < 0.001 ( t- test). ( C ) The expression levels of PD-L1 mRNA in A549 and H292 cells were detected after GA treatment in concentrations indicated for 48 hours. The relative expression levels of PD-L1 mRNA were determined by real time qPCR and normalized to GAPDH mRNA. Data are representative of three independent experiments. *** p < 0.001 ( t- test).

Journal: Cancers

Article Title: The Inhibitory Mechanisms of Tumor PD-L1 Expression by Natural Bioactive Gallic Acid in Non-Small-Cell Lung Cancer (NSCLC) Cells

doi: 10.3390/cancers12030727

Figure Lengend Snippet: Gallic acid (GA) reduces the programmed death-ligand 1 (PD-L1) expression in non-small-cell lung cancer (NSCLC) cells. ( A ) The expression levels of PD-L1 protein in A549 and H292 cells were detected after GA treatment in concentrations indicated for 48 hours. ( B ) The relative expression levels of PD-L1 protein were determined by densitometry and normalized to β-actin. Data are representative of three independent experiments. *** p < 0.001 ( t- test). ( C ) The expression levels of PD-L1 mRNA in A549 and H292 cells were detected after GA treatment in concentrations indicated for 48 hours. The relative expression levels of PD-L1 mRNA were determined by real time qPCR and normalized to GAPDH mRNA. Data are representative of three independent experiments. *** p < 0.001 ( t- test).

Article Snippet: PD-L1 (R30949) antibody was purchased from NSJ Bioreagents (San Diego, CA, USA).

Techniques: Expressing

GA increased p53 expression. ( A ) The expression levels of p53 protein in A549 and H292 cells were detected after GA treatment in concentrations indicated for 48 hours. ( B ) The relative expression levels of p53 protein were determined by densitometry and normalized to β-actin. Data are representative of three independent experiments. * p < 0.05 and ** p < 0.01 ( t- test). ( C ) The expression levels of p53 mRNA in A549 and H292 cells were detected by real time qPCR after GA treatment in concentrations indicated for 48 hours. The relative expression levels of p53 mRNA were determined and normalized to GAPDH mRNA. Data are representative of three independent experiments. * p < 0.05 and *** p < 0.001 ( t- test). ( D ) The expression levels of p53 and PD-L1 protein in A549 and H292 cells, treated with GA (A549: 400 μM; H292: 100 μM) or 30 pM p53 siRNA, were detected by western blotting at 48 hours. ( E ) The relative expression levels of p53 and PD-L1 protein were determined using densitometry and normalized to β-actin. Data are representative of three independent experiments. *** p < 0.001 (ANOVA); # p < 0.001 vs. control.

Journal: Cancers

Article Title: The Inhibitory Mechanisms of Tumor PD-L1 Expression by Natural Bioactive Gallic Acid in Non-Small-Cell Lung Cancer (NSCLC) Cells

doi: 10.3390/cancers12030727

Figure Lengend Snippet: GA increased p53 expression. ( A ) The expression levels of p53 protein in A549 and H292 cells were detected after GA treatment in concentrations indicated for 48 hours. ( B ) The relative expression levels of p53 protein were determined by densitometry and normalized to β-actin. Data are representative of three independent experiments. * p < 0.05 and ** p < 0.01 ( t- test). ( C ) The expression levels of p53 mRNA in A549 and H292 cells were detected by real time qPCR after GA treatment in concentrations indicated for 48 hours. The relative expression levels of p53 mRNA were determined and normalized to GAPDH mRNA. Data are representative of three independent experiments. * p < 0.05 and *** p < 0.001 ( t- test). ( D ) The expression levels of p53 and PD-L1 protein in A549 and H292 cells, treated with GA (A549: 400 μM; H292: 100 μM) or 30 pM p53 siRNA, were detected by western blotting at 48 hours. ( E ) The relative expression levels of p53 and PD-L1 protein were determined using densitometry and normalized to β-actin. Data are representative of three independent experiments. *** p < 0.001 (ANOVA); # p < 0.001 vs. control.

Article Snippet: PD-L1 (R30949) antibody was purchased from NSJ Bioreagents (San Diego, CA, USA).

Techniques: Expressing, Western Blot

GA upregulates the PD-L1 expression by p53 via miR-34a. ( A ) Relative expression levels of miR34a after treatment of GA (A549: 400 μM; H292: 100 μM) for 24 and 48 hours. Data are representative of three independent experiments. ** p < 0.01 (ANOVA); & p < 0.01 vs. control. ( B ) The expression levels of miR-34a in A549 and H292 cells, treated with GA (A549: 400 μM; H292: 100 μM), p53 siRNA (60 pM) or GA plus p53 siRNA were detected by RT-PCR at 48 hours. The relative expression levels of miR-34a were determined using densitometry and normalized to U6. Data are representative of three independent experiments. *** p < 0.001 (ANOVA); # p < 0.001 vs. control. ( C ) The expression levels of p53 and PD-L1 protein in A549 and H292 cells, treated with GA (A549: 400 μM; H292: 100 μM) or 30 pM miR-34a inhibitor, were detected by western blotting at 48 hours. ( D ) The relative expression levels of p53 and PD-L1 protein were determined by densitometry and normalized to β-actin. Data are representative of three independent experiments. *** p < 0.001 (ANOVA); # p < 0.001 vs. control.

Journal: Cancers

Article Title: The Inhibitory Mechanisms of Tumor PD-L1 Expression by Natural Bioactive Gallic Acid in Non-Small-Cell Lung Cancer (NSCLC) Cells

doi: 10.3390/cancers12030727

Figure Lengend Snippet: GA upregulates the PD-L1 expression by p53 via miR-34a. ( A ) Relative expression levels of miR34a after treatment of GA (A549: 400 μM; H292: 100 μM) for 24 and 48 hours. Data are representative of three independent experiments. ** p < 0.01 (ANOVA); & p < 0.01 vs. control. ( B ) The expression levels of miR-34a in A549 and H292 cells, treated with GA (A549: 400 μM; H292: 100 μM), p53 siRNA (60 pM) or GA plus p53 siRNA were detected by RT-PCR at 48 hours. The relative expression levels of miR-34a were determined using densitometry and normalized to U6. Data are representative of three independent experiments. *** p < 0.001 (ANOVA); # p < 0.001 vs. control. ( C ) The expression levels of p53 and PD-L1 protein in A549 and H292 cells, treated with GA (A549: 400 μM; H292: 100 μM) or 30 pM miR-34a inhibitor, were detected by western blotting at 48 hours. ( D ) The relative expression levels of p53 and PD-L1 protein were determined by densitometry and normalized to β-actin. Data are representative of three independent experiments. *** p < 0.001 (ANOVA); # p < 0.001 vs. control.

Article Snippet: PD-L1 (R30949) antibody was purchased from NSJ Bioreagents (San Diego, CA, USA).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

Molecular regulatory mechanism of programmed death-ligand 1 (PD-L1) by natural bioactive gallic acid in NSCLC cells and proposed combination effect for NSCLC immunotherapy.

Journal: Cancers

Article Title: The Inhibitory Mechanisms of Tumor PD-L1 Expression by Natural Bioactive Gallic Acid in Non-Small-Cell Lung Cancer (NSCLC) Cells

doi: 10.3390/cancers12030727

Figure Lengend Snippet: Molecular regulatory mechanism of programmed death-ligand 1 (PD-L1) by natural bioactive gallic acid in NSCLC cells and proposed combination effect for NSCLC immunotherapy.

Article Snippet: PD-L1 (R30949) antibody was purchased from NSJ Bioreagents (San Diego, CA, USA).

Techniques: