R32494 Search Results


phorbol 12 myristate 13 acetate  (Shanghai Yuanye Biochemicals)
86
Shanghai Yuanye Biochemicals phorbol 12 myristate 13 acetate
TTP suppressed T helper 2 (Th2)-type inflammation in AR mice. A , schematics of splenocytes isolation from AR-mice and the differentiation of splenic naive CD4+ T cells model in vitro . B , the levels of Interleukin (IL)-4, IL-5, and IL-13, which belong to the Th2 cytokine family, were measured by ELISA assays in the cell supernatant. The cell supernatant was collected from mononuclear cells of the spleen that had been treated with ionomycin and <t>phorbol</t> <t>12-myristate</t> <t>13-acetate</t> (PMA) for 6 h. C , after treatment with ionomycin, PMA, and GolgiPlug, the cells with the double stain of anti-CD4 and anti-IL-4-APC were detected by flow cytometry to analyze the percentage of Th2 cells. Data are shown as means ± SD (N = 3 or 6). One-way analysis of variance (ANOVA) was used for statistical analysis of control mice, AR mice, and AR mice transduced with control vectors or TTP overexpression vectors.
Phorbol 12 Myristate 13 Acetate, supplied by Shanghai Yuanye Biochemicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phorbol 12 myristate 13 acetate/product/Shanghai Yuanye Biochemicals
Average 86 stars, based on 1 article reviews
phorbol 12 myristate 13 acetate - by Bioz Stars, 2026-05
86/100 stars
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antkmt human sirna oligo duplex  (OriGene)
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FAM173A Human 3 unique 27mer siRNA duplexes 2 nmol each
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fcrl2 human sirna oligo duplex  (OriGene)
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FCRL2 Human 3 unique 27mer siRNA duplexes 2 nmol each
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mmp9 antibody / matrix metalloproteinase-9  (NSJ Bioreagents)
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Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is an enzyme that in humans is encoded by the MMP9 gene. Proteins of the matrix
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adamts13 antibody  (NSJ Bioreagents)
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ADAMTS13 (A disintegrin and metalloproteinase with athrombospondin type 1 motif, member 13), also known as VWFCP, is a zinc-containing metalloprotease enzyme that cleaves von Willebrand factor (vWf), a large protein involved in blood clotting. It
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ada2 human sirna oligo duplex  (OriGene)
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CECR1 Human 3 unique 27mer siRNA duplexes 2 nmol each
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tmem39a human sirna oligo duplex  (OriGene)
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TMEM39A Human 3 unique 27mer siRNA duplexes 2 nmol each
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fbxw7 human sirna oligo duplex  (OriGene)
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FBXW7 Human 3 unique 27mer siRNA duplexes 2 nmol each
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bap29 bcap29 human sirna oligo duplex  (OriGene)
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BCAP29 Human 3 unique 27mer siRNA duplexes 2 nmol each
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pf4 antibody / platelet factor 4  (NSJ Bioreagents)
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Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). By in situ hybridization, the CXCL4 gene is mapped to
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irak4 human sirna oligo duplex  (OriGene)
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IRAK4 Human 3 unique 27mer siRNA duplexes 2 nmol each
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yipf1 human sirna oligo duplex  (OriGene)
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YIPF1 Human 3 unique 27mer siRNA duplexes 2 nmol each
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Image Search Results


TTP suppressed T helper 2 (Th2)-type inflammation in AR mice. A , schematics of splenocytes isolation from AR-mice and the differentiation of splenic naive CD4+ T cells model in vitro . B , the levels of Interleukin (IL)-4, IL-5, and IL-13, which belong to the Th2 cytokine family, were measured by ELISA assays in the cell supernatant. The cell supernatant was collected from mononuclear cells of the spleen that had been treated with ionomycin and phorbol 12-myristate 13-acetate (PMA) for 6 h. C , after treatment with ionomycin, PMA, and GolgiPlug, the cells with the double stain of anti-CD4 and anti-IL-4-APC were detected by flow cytometry to analyze the percentage of Th2 cells. Data are shown as means ± SD (N = 3 or 6). One-way analysis of variance (ANOVA) was used for statistical analysis of control mice, AR mice, and AR mice transduced with control vectors or TTP overexpression vectors.

Journal: The Journal of Biological Chemistry

Article Title: RNA-binding protein tristetraprolin inhibits Th2 cell activation and differentiation in allergic rhinitis by promoting TRIM18 mRNA decay

doi: 10.1016/j.jbc.2026.111240

Figure Lengend Snippet: TTP suppressed T helper 2 (Th2)-type inflammation in AR mice. A , schematics of splenocytes isolation from AR-mice and the differentiation of splenic naive CD4+ T cells model in vitro . B , the levels of Interleukin (IL)-4, IL-5, and IL-13, which belong to the Th2 cytokine family, were measured by ELISA assays in the cell supernatant. The cell supernatant was collected from mononuclear cells of the spleen that had been treated with ionomycin and phorbol 12-myristate 13-acetate (PMA) for 6 h. C , after treatment with ionomycin, PMA, and GolgiPlug, the cells with the double stain of anti-CD4 and anti-IL-4-APC were detected by flow cytometry to analyze the percentage of Th2 cells. Data are shown as means ± SD (N = 3 or 6). One-way analysis of variance (ANOVA) was used for statistical analysis of control mice, AR mice, and AR mice transduced with control vectors or TTP overexpression vectors.

Article Snippet: The splenocytes were incubated in RPMI 1640 medium containing 10% fetal bovine serum, 50 ng/ml phorbol 12-myristate 13-acetate (PMA; Shanghai Yuanye Bio-Technology, Cat No. R32414 , China) and 1 μg/ml ionomycin (Macklin, Cat No.I838446, China), at 37 °C and 5% CO 2 .

Techniques: Isolation, In Vitro, Enzyme-linked Immunosorbent Assay, Staining, Flow Cytometry, Control, Transduction, Over Expression