Journal: PLoS Pathogens

Article Title: Patient genetics is linked to chronic wound microbiome composition and healing

doi: 10.1371/journal.ppat.1008511

Figure Lengend Snippet: a , Boxplot of Hill 1 diversity as a function of genotype at rs8031916 ( TLN2 ) and rs7236481 ( ZNF521 ), each explaining significant components of variation in the two-cohort mbGWAS. b , Distance-based redundancy analysis based on Bray-Curtis dissimilarities with relative abundance species effects indicated by vectors. rs8031916 significantly explained beta diversity (p < 0.01). The distribution of genotypes at rs8031916 across MDS1 and MDS2 are illustrated by boxplots. c , Boxplot illustrating how residual relative abundances of P . aeruginosa and S . epidermidis were significantly explained by rs8031916 (p < 0.05 for each). d , Communities in which P . aeruginosa (Pa) was present were significantly less diverse than communities in which S . epidermidis occurred (p < 0.05).

Article Snippet: Tln2 was localized with a combination of 2 μl/ml Rabbit anti-TLN2 antibody (Cat: R32413, NSJ Bioreagents, San Diego, CA) incubated overnight at 4°C, followed by 2 μl/ml Donkey anti-rabbit Alexa Fluor 488 (Cat: A21206, Life Technologies, Eugene, OR) antibody incubated for 1 hour at room temperature.

Techniques:

Journal: PLoS Pathogens

Article Title: Patient genetics is linked to chronic wound microbiome composition and healing

doi: 10.1371/journal.ppat.1008511

Figure Lengend Snippet: a, Select ontological terms that were enriched in this study. See for a full list of terms. Terms involving TLN2 are colored yellow and number of included genes (out of 15 total included in testing) per term is given in parentheses next to each term. b, Distribution of TLN2 protein coding transcripts on human chromosome 15 with functional domains illustrated per Gough and Goult 2018. Red shading, from left to right denotes the transcript regions amplified from either the canonical isoform, three isoform, or four isoform assay used to compare alternative transcription by genotype (see ). c, Standard curve of RT-qPCR assays, with calculated efficiencies shown per assay. d, TLN2 log isoform ratios (calculated as the log of either the three or four isoform Ct divided by the canonical Ct) were significantly associated by rs8031916 genotype. Here, two levels of pairwise comparison are shown: above the dashed line are overall genotype comparisons, while within-genotype assay comparisons are indicated below the dashed line. Significance annotation is as follows: ns = (p>0.05), * = (p<0.05), and ** = (p<0.01).

Article Snippet: Tln2 was localized with a combination of 2 μl/ml Rabbit anti-TLN2 antibody (Cat: R32413, NSJ Bioreagents, San Diego, CA) incubated overnight at 4°C, followed by 2 μl/ml Donkey anti-rabbit Alexa Fluor 488 (Cat: A21206, Life Technologies, Eugene, OR) antibody incubated for 1 hour at room temperature.

Techniques: Functional Assay, Amplification, Quantitative RT-PCR

Journal: PLoS Pathogens

Article Title: Patient genetics is linked to chronic wound microbiome composition and healing

doi: 10.1371/journal.ppat.1008511

Figure Lengend Snippet: Tln2 was observed in adipocytes (a, b, c), and striated muscle (d, e, f). H&E staining (a, d), IHC negative control (b, e), IHC detection of Tln2 with Alexa Fluor 488 secondary (c, f). Nuclei were stained with DAPI. Scale bars are given for each image.

Article Snippet: Tln2 was localized with a combination of 2 μl/ml Rabbit anti-TLN2 antibody (Cat: R32413, NSJ Bioreagents, San Diego, CA) incubated overnight at 4°C, followed by 2 μl/ml Donkey anti-rabbit Alexa Fluor 488 (Cat: A21206, Life Technologies, Eugene, OR) antibody incubated for 1 hour at room temperature.

Techniques: Staining, Negative Control