R32165 Search Results


s glycines  (ATCC)
94
ATCC s glycines
S Glycines, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/s glycines/product/ATCC
Average 94 stars, based on 1 article reviews
s glycines - by Bioz Stars, 2026-04
94/100 stars
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ngal  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology ngal
Figure 3. Intracellular localization of SOD1 in neutrophils. (A) Representative immunofluorescence images of human neutrophils stained <t>with</t> <t>antibodies</t> against NE (red), SOD1 (green), C. albicans (blue) or S. aureus (magenta). (B) Transmission electron microscopy of naïve human neutrophils. Large gold nano-particles indicate SOD1 and small gold nano-particles indicate MPO. Bars correspond to 2.5 μm (A) and 1 μm (B). (C) Intragranular SOD1 localization. Lysed neutrophils by nitrogen cavitation (whole lysate, PMN WL) were subjected to subcellular fractionation by using Percoll density gradient centrifugation. The obtained fractions representing azurophilic, specific, gelatinase granules, and secretory vesicles were analyzed by Western blotting. Antibodies directed against MPO (azurophilic granules), <t>NGAL</t> (specific granules), MMP9 (gelatinase granules) and HSA (secretory vesicles) were used to evaluate fractionation (N ¼ 3). An equal amount of protein from each fraction was loaded. (D) Degranulation of SOD1. Human neutrophils were stimulated with PMA (50 nM) or C. albicans (MOI 1:1) and collected cell culture supernatants analyzed for the presence of SOD1 and MPO by Western blotting. Positive control represents whole neutrophil lysate (PMN WL).
Ngal, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ngal/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
ngal - by Bioz Stars, 2026-04
93/100 stars
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polyol r3430  (Perstorp Chemicals GmbH)
90
Perstorp Chemicals GmbH polyol r3430
Figure 3. Intracellular localization of SOD1 in neutrophils. (A) Representative immunofluorescence images of human neutrophils stained <t>with</t> <t>antibodies</t> against NE (red), SOD1 (green), C. albicans (blue) or S. aureus (magenta). (B) Transmission electron microscopy of naïve human neutrophils. Large gold nano-particles indicate SOD1 and small gold nano-particles indicate MPO. Bars correspond to 2.5 μm (A) and 1 μm (B). (C) Intragranular SOD1 localization. Lysed neutrophils by nitrogen cavitation (whole lysate, PMN WL) were subjected to subcellular fractionation by using Percoll density gradient centrifugation. The obtained fractions representing azurophilic, specific, gelatinase granules, and secretory vesicles were analyzed by Western blotting. Antibodies directed against MPO (azurophilic granules), <t>NGAL</t> (specific granules), MMP9 (gelatinase granules) and HSA (secretory vesicles) were used to evaluate fractionation (N ¼ 3). An equal amount of protein from each fraction was loaded. (D) Degranulation of SOD1. Human neutrophils were stimulated with PMA (50 nM) or C. albicans (MOI 1:1) and collected cell culture supernatants analyzed for the presence of SOD1 and MPO by Western blotting. Positive control represents whole neutrophil lysate (PMN WL).
Polyol R3430, supplied by Perstorp Chemicals GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyol r3430/product/Perstorp Chemicals GmbH
Average 90 stars, based on 1 article reviews
polyol r3430 - by Bioz Stars, 2026-04
90/100 stars
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segment software  (Medviso AB)
90
Medviso AB segment software
Figure 3. Intracellular localization of SOD1 in neutrophils. (A) Representative immunofluorescence images of human neutrophils stained <t>with</t> <t>antibodies</t> against NE (red), SOD1 (green), C. albicans (blue) or S. aureus (magenta). (B) Transmission electron microscopy of naïve human neutrophils. Large gold nano-particles indicate SOD1 and small gold nano-particles indicate MPO. Bars correspond to 2.5 μm (A) and 1 μm (B). (C) Intragranular SOD1 localization. Lysed neutrophils by nitrogen cavitation (whole lysate, PMN WL) were subjected to subcellular fractionation by using Percoll density gradient centrifugation. The obtained fractions representing azurophilic, specific, gelatinase granules, and secretory vesicles were analyzed by Western blotting. Antibodies directed against MPO (azurophilic granules), <t>NGAL</t> (specific granules), MMP9 (gelatinase granules) and HSA (secretory vesicles) were used to evaluate fractionation (N ¼ 3). An equal amount of protein from each fraction was loaded. (D) Degranulation of SOD1. Human neutrophils were stimulated with PMA (50 nM) or C. albicans (MOI 1:1) and collected cell culture supernatants analyzed for the presence of SOD1 and MPO by Western blotting. Positive control represents whole neutrophil lysate (PMN WL).
Segment Software, supplied by Medviso AB, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/segment software/product/Medviso AB
Average 90 stars, based on 1 article reviews
segment software - by Bioz Stars, 2026-04
90/100 stars
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razoxane  (LGC LoGiCal)
N/A
Razoxane
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ctbp2 antibody  (NSJ Bioreagents)
N/A
The E1a region of group C adenoviruses encodes 2 nearly identical proteins that are largely responsible for the oncogenic properties of adenoviruses. The CTBP1 protein binds to the C-terminal half of these E1A proteins. It's
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rab10 antibody  (NSJ Bioreagents)
N/A
Ras-related protein Rab-10 is a protein that in humans is encoded by the RAB10 gene. RAB10 belongs to the RAS superfamily of small GTPases. And RAB proteins localize to exocytic and endocytic compartments and regulate
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rac1 antibody  (NSJ Bioreagents)
N/A
RAC1, also known as Ras-related C3 botulinum toxin substrate 1, is a protein found in human cells. It is encoded by the RAC1 gene. This gene can produce a variety of alternatively splicedversions of the
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yb1 antibody  (NSJ Bioreagents)
N/A
YBX1 (Y box binding protein 1), commonly referred to as YB-1 by researchers, is a human protein. YB1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box. It
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arms2 human shrna plasmid kit  (OriGene)
N/A
ARMS2 Human 4 unique 29mer shRNA constructs in retroviral untagged vector
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rhoxf2b human shrna plasmid kit  (OriGene)
N/A
RHOXF2B Human 4 unique 29mer shRNA constructs in retroviral untagged vector
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slc35e2b human shrna plasmid kit  (OriGene)
N/A
SLC35E2B Human 4 unique 29mer shRNA constructs in retroviral untagged vector
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Image Search Results


Figure 3. Intracellular localization of SOD1 in neutrophils. (A) Representative immunofluorescence images of human neutrophils stained with antibodies against NE (red), SOD1 (green), C. albicans (blue) or S. aureus (magenta). (B) Transmission electron microscopy of naïve human neutrophils. Large gold nano-particles indicate SOD1 and small gold nano-particles indicate MPO. Bars correspond to 2.5 μm (A) and 1 μm (B). (C) Intragranular SOD1 localization. Lysed neutrophils by nitrogen cavitation (whole lysate, PMN WL) were subjected to subcellular fractionation by using Percoll density gradient centrifugation. The obtained fractions representing azurophilic, specific, gelatinase granules, and secretory vesicles were analyzed by Western blotting. Antibodies directed against MPO (azurophilic granules), NGAL (specific granules), MMP9 (gelatinase granules) and HSA (secretory vesicles) were used to evaluate fractionation (N ¼ 3). An equal amount of protein from each fraction was loaded. (D) Degranulation of SOD1. Human neutrophils were stimulated with PMA (50 nM) or C. albicans (MOI 1:1) and collected cell culture supernatants analyzed for the presence of SOD1 and MPO by Western blotting. Positive control represents whole neutrophil lysate (PMN WL).

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: SOD1 controls neutrophil oxidative burst and microbial killing.

doi: 10.1093/jimmun/vkaf110

Figure Lengend Snippet: Figure 3. Intracellular localization of SOD1 in neutrophils. (A) Representative immunofluorescence images of human neutrophils stained with antibodies against NE (red), SOD1 (green), C. albicans (blue) or S. aureus (magenta). (B) Transmission electron microscopy of naïve human neutrophils. Large gold nano-particles indicate SOD1 and small gold nano-particles indicate MPO. Bars correspond to 2.5 μm (A) and 1 μm (B). (C) Intragranular SOD1 localization. Lysed neutrophils by nitrogen cavitation (whole lysate, PMN WL) were subjected to subcellular fractionation by using Percoll density gradient centrifugation. The obtained fractions representing azurophilic, specific, gelatinase granules, and secretory vesicles were analyzed by Western blotting. Antibodies directed against MPO (azurophilic granules), NGAL (specific granules), MMP9 (gelatinase granules) and HSA (secretory vesicles) were used to evaluate fractionation (N ¼ 3). An equal amount of protein from each fraction was loaded. (D) Degranulation of SOD1. Human neutrophils were stimulated with PMA (50 nM) or C. albicans (MOI 1:1) and collected cell culture supernatants analyzed for the presence of SOD1 and MPO by Western blotting. Positive control represents whole neutrophil lysate (PMN WL).

Article Snippet: Western blot was used to analyze the protein contents, using the following antibodies: SOD1 (MAB3418, Biotechne), MPO (4A4 sc-52076, Santa Cruz), NGAL (R32169, NSJ), MMP9 (E-11 sc-393859, Santa Cruz) and HSA (MA119174, Invitrogen).

Techniques: Immunofluorescence, Staining, Transmission Assay, Electron Microscopy, Fractionation, Gradient Centrifugation, Western Blot, Cell Culture, Positive Control