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NSJ Bioreagents
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Journal: Hepatology Communications
Article Title: Constitutive Androstane Receptor Differentially Regulates Bile Acid Homeostasis in Mouse Models of Intrahepatic Cholestasis
doi: 10.1002/hep4.1274
Figure Lengend Snippet: TKO further attenuates ABCB11‐mediated fecal BA excretion. (A) mRNA (n = 8) and (B) protein levels (n = 3) of ABCB11 were analyzed by q‐PCR and proteomics, respectively. Ratio (%) indicates the proportion of ABCB11 expressions compared to WT. (C) Immunostaining of ABCB11 (magnification ×200); arrowhead, residual canalicular ABCB11 expression. (D) BA levels in gallbladder‐collected bile (n = 4) and feces (n = 3). ANOVA followed by Tukey HSD, # P < 0.05, ### P < 0.005. Abbreviation: iFOT, intensity‐based fraction of total.
Article Snippet: Briefly, after antigen retrieval in sodium citrate buffer (pH 6.0), sections were incubated with blocking buffer (5% normal goat serum, 30 minutes) followed by diluted anti‐KRT19 (1:200, Ab133496; Abcam; Research Resource Identifier [RRID], AB_11155282),
Techniques: Immunostaining, Expressing
Journal: Hepatology Communications
Article Title: Constitutive Androstane Receptor Differentially Regulates Bile Acid Homeostasis in Mouse Models of Intrahepatic Cholestasis
doi: 10.1002/hep4.1274
Figure Lengend Snippet: TC treatment enhances canalicular and basolateral BA transporters. (A) Fecal BA excretion (n = 4) and urinary BA concentration (n = 3) in TC‐treated (1 week) DKO and BKO. (B) mRNA levels of BA transporters (n = 4). (C) Immunostaining of ABCB11, ABCC3, and ABCC4 (magnification ×200). Arrowhead, positive area. Student t test; * P < 0.05, *** P < 0.005 compared to CO‐treated group. Abbreviation: MDR, multidrug resistance.
Article Snippet: Briefly, after antigen retrieval in sodium citrate buffer (pH 6.0), sections were incubated with blocking buffer (5% normal goat serum, 30 minutes) followed by diluted anti‐KRT19 (1:200, Ab133496; Abcam; Research Resource Identifier [RRID], AB_11155282),
Techniques: Concentration Assay, Immunostaining