R31802 Search Results


sphi hf  (New England Biolabs)
96
New England Biolabs sphi hf
Genetic confirmation of complementation <t>of</t> <t>senX3</t> ::Tn and regX3 ::Tn. (A) . Diagram of a 2824-bp fragment containing the senX3-regX3 operon from the genome of wild-type CDC1551. (B) FseI digestion of genomic DNA and hybridization with dig-labeled senX3 probe reveals fragments measuring 3.7-kb and 5.7-kb in CDC1551 and senX 3::Tn, respectively, as well as 5.7-kb and 7.7-kb fragments in senX3 ::Tn Comp. (C) <t>SphI</t> digestion of genomic DNA followed by hybridization with dig-labeled regX3 probe reveals 5.5-kb and 7.5-kb fragments in CDC1551 and regX3 ::Tn, respectively, as well as 7.5-kb and 4.6-kb fragments in regX3 ::Tn Comp. (D) Southern analysis of senX3 complementation. Lanes 1 and 2: SenX3 ::Tn Comp candidates with appropriately-sized bands; Lane 3: SenX 3::Tn; Lane 4: Wild-type CDC1551. (E) Southern analysis of regX3 complementation. Lane 1: Wild-type CDC551; Lane 2: regX 3::Tn; Lanes 3–5: regX3 ::Tn Comp candidates with appropriately-sized bands.
Sphi Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sphi hf/product/New England Biolabs
Average 96 stars, based on 1 article reviews
sphi hf - by Bioz Stars, 2026-03
96/100 stars
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4 4 kb dna  (New England Biolabs)
96
New England Biolabs 4 4 kb dna
Human Exo1-biotin (hExo1-bio) purification and labeling. (A) Purification scheme for hExo1-bio. (B) SDS/PAGE gel showing hExo1-bio and hExo1-bio + streptavidin. Gel shift of hExo1-bio-streptavidin conjugates are indicated. The complete disappearance of the hExo1-bio band indicates that nearly 100% of the purified nucleases are biotinylated. (C) Resection assay with untagged WT hExo1 (500 pM), hExo1-bio (500 pM), or hExo1-bio (500 pM) + streptavidin (1 µg). Proteins were incubated in imaging buffer with 30 ng linearized <t>4.4-kb</t> <t>DNA</t> (4-nt 3′ overhang) for 30 min at 37 °C. Resected DNA was separated on a 1% agarose gel and stained with SYBR green. The resection protocol has also been described previously (21). Together, these assays indicate that streptavidin-conjugated hExo1-bio retains full resection activity. (D) Snapshots at indicated times (Upper) and single-particle tracking of two representative trajectories of resection by CF488-anti-biotin–labeled hExo1 (Lower). In both trajectories, hExo1 transitions between a resecting and a paused state. These results indicate that both states are intrinsic to hExo1 and are not dependent on the nature of the fluorophore.
4 4 Kb Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/4 4 kb dna/product/New England Biolabs
Average 96 stars, based on 1 article reviews
4 4 kb dna - by Bioz Stars, 2026-03
96/100 stars
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sphi  (New England Biolabs)
96
New England Biolabs sphi
Human Exo1-biotin (hExo1-bio) purification and labeling. (A) Purification scheme for hExo1-bio. (B) SDS/PAGE gel showing hExo1-bio and hExo1-bio + streptavidin. Gel shift of hExo1-bio-streptavidin conjugates are indicated. The complete disappearance of the hExo1-bio band indicates that nearly 100% of the purified nucleases are biotinylated. (C) Resection assay with untagged WT hExo1 (500 pM), hExo1-bio (500 pM), or hExo1-bio (500 pM) + streptavidin (1 µg). Proteins were incubated in imaging buffer with 30 ng linearized <t>4.4-kb</t> <t>DNA</t> (4-nt 3′ overhang) for 30 min at 37 °C. Resected DNA was separated on a 1% agarose gel and stained with SYBR green. The resection protocol has also been described previously (21). Together, these assays indicate that streptavidin-conjugated hExo1-bio retains full resection activity. (D) Snapshots at indicated times (Upper) and single-particle tracking of two representative trajectories of resection by CF488-anti-biotin–labeled hExo1 (Lower). In both trajectories, hExo1 transitions between a resecting and a paused state. These results indicate that both states are intrinsic to hExo1 and are not dependent on the nature of the fluorophore.
Sphi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sphi/product/New England Biolabs
Average 96 stars, based on 1 article reviews
sphi - by Bioz Stars, 2026-03
96/100 stars
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spectrum analyzer  (Advantest America Inc)
90
Advantest America Inc spectrum analyzer
Human Exo1-biotin (hExo1-bio) purification and labeling. (A) Purification scheme for hExo1-bio. (B) SDS/PAGE gel showing hExo1-bio and hExo1-bio + streptavidin. Gel shift of hExo1-bio-streptavidin conjugates are indicated. The complete disappearance of the hExo1-bio band indicates that nearly 100% of the purified nucleases are biotinylated. (C) Resection assay with untagged WT hExo1 (500 pM), hExo1-bio (500 pM), or hExo1-bio (500 pM) + streptavidin (1 µg). Proteins were incubated in imaging buffer with 30 ng linearized <t>4.4-kb</t> <t>DNA</t> (4-nt 3′ overhang) for 30 min at 37 °C. Resected DNA was separated on a 1% agarose gel and stained with SYBR green. The resection protocol has also been described previously (21). Together, these assays indicate that streptavidin-conjugated hExo1-bio retains full resection activity. (D) Snapshots at indicated times (Upper) and single-particle tracking of two representative trajectories of resection by CF488-anti-biotin–labeled hExo1 (Lower). In both trajectories, hExo1 transitions between a resecting and a paused state. These results indicate that both states are intrinsic to hExo1 and are not dependent on the nature of the fluorophore.
Spectrum Analyzer, supplied by Advantest America Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spectrum analyzer/product/Advantest America Inc
Average 90 stars, based on 1 article reviews
spectrum analyzer - by Bioz Stars, 2026-03
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cutsmart buffer  (New England Biolabs)
86
New England Biolabs cutsmart buffer
Human Exo1-biotin (hExo1-bio) purification and labeling. (A) Purification scheme for hExo1-bio. (B) SDS/PAGE gel showing hExo1-bio and hExo1-bio + streptavidin. Gel shift of hExo1-bio-streptavidin conjugates are indicated. The complete disappearance of the hExo1-bio band indicates that nearly 100% of the purified nucleases are biotinylated. (C) Resection assay with untagged WT hExo1 (500 pM), hExo1-bio (500 pM), or hExo1-bio (500 pM) + streptavidin (1 µg). Proteins were incubated in imaging buffer with 30 ng linearized <t>4.4-kb</t> <t>DNA</t> (4-nt 3′ overhang) for 30 min at 37 °C. Resected DNA was separated on a 1% agarose gel and stained with SYBR green. The resection protocol has also been described previously (21). Together, these assays indicate that streptavidin-conjugated hExo1-bio retains full resection activity. (D) Snapshots at indicated times (Upper) and single-particle tracking of two representative trajectories of resection by CF488-anti-biotin–labeled hExo1 (Lower). In both trajectories, hExo1 transitions between a resecting and a paused state. These results indicate that both states are intrinsic to hExo1 and are not dependent on the nature of the fluorophore.
Cutsmart Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cutsmart buffer/product/New England Biolabs
Average 86 stars, based on 1 article reviews
cutsmart buffer - by Bioz Stars, 2026-03
86/100 stars
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sphi neb r3182 ecori  (New England Biolabs)
99
New England Biolabs sphi neb r3182 ecori
Human Exo1-biotin (hExo1-bio) purification and labeling. (A) Purification scheme for hExo1-bio. (B) SDS/PAGE gel showing hExo1-bio and hExo1-bio + streptavidin. Gel shift of hExo1-bio-streptavidin conjugates are indicated. The complete disappearance of the hExo1-bio band indicates that nearly 100% of the purified nucleases are biotinylated. (C) Resection assay with untagged WT hExo1 (500 pM), hExo1-bio (500 pM), or hExo1-bio (500 pM) + streptavidin (1 µg). Proteins were incubated in imaging buffer with 30 ng linearized <t>4.4-kb</t> <t>DNA</t> (4-nt 3′ overhang) for 30 min at 37 °C. Resected DNA was separated on a 1% agarose gel and stained with SYBR green. The resection protocol has also been described previously (21). Together, these assays indicate that streptavidin-conjugated hExo1-bio retains full resection activity. (D) Snapshots at indicated times (Upper) and single-particle tracking of two representative trajectories of resection by CF488-anti-biotin–labeled hExo1 (Lower). In both trajectories, hExo1 transitions between a resecting and a paused state. These results indicate that both states are intrinsic to hExo1 and are not dependent on the nature of the fluorophore.
Sphi Neb R3182 Ecori, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sphi neb r3182 ecori/product/New England Biolabs
Average 99 stars, based on 1 article reviews
sphi neb r3182 ecori - by Bioz Stars, 2026-03
99/100 stars
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rock2 antibody  (NSJ Bioreagents)
N/A
Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one
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znf283 human shrna plasmid kit  (OriGene)
N/A
ZNF283 Human 4 unique 29mer shRNA constructs in retroviral untagged vector
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znf844 human shrna plasmid kit  (OriGene)
N/A
ZNF844 Human 4 unique 29mer shRNA constructs in retroviral untagged vector
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c1orf104 rusc1 as1 human shrna plasmid kit  (OriGene)
N/A
RUSC1 AS1 Human 4 unique 29mer shRNA constructs in retroviral untagged vector
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rgpd4 human shrna plasmid kit  (OriGene)
N/A
RGPD4 Human 4 unique 29mer shRNA constructs in retroviral untagged vector
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flj90723 human shrna plasmid kit  (OriGene)
N/A
FLJ90723 Human 4 unique 29mer shRNA constructs in retroviral untagged vector
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Image Search Results


Genetic confirmation of complementation of senX3 ::Tn and regX3 ::Tn. (A) . Diagram of a 2824-bp fragment containing the senX3-regX3 operon from the genome of wild-type CDC1551. (B) FseI digestion of genomic DNA and hybridization with dig-labeled senX3 probe reveals fragments measuring 3.7-kb and 5.7-kb in CDC1551 and senX 3::Tn, respectively, as well as 5.7-kb and 7.7-kb fragments in senX3 ::Tn Comp. (C) SphI digestion of genomic DNA followed by hybridization with dig-labeled regX3 probe reveals 5.5-kb and 7.5-kb fragments in CDC1551 and regX3 ::Tn, respectively, as well as 7.5-kb and 4.6-kb fragments in regX3 ::Tn Comp. (D) Southern analysis of senX3 complementation. Lanes 1 and 2: SenX3 ::Tn Comp candidates with appropriately-sized bands; Lane 3: SenX 3::Tn; Lane 4: Wild-type CDC1551. (E) Southern analysis of regX3 complementation. Lane 1: Wild-type CDC551; Lane 2: regX 3::Tn; Lanes 3–5: regX3 ::Tn Comp candidates with appropriately-sized bands.

Journal: BMC Microbiology

Article Title: senX3 -independent contribution of regX3 to Mycobacterium tuberculosis virulence

doi: 10.1186/s12866-014-0265-8

Figure Lengend Snippet: Genetic confirmation of complementation of senX3 ::Tn and regX3 ::Tn. (A) . Diagram of a 2824-bp fragment containing the senX3-regX3 operon from the genome of wild-type CDC1551. (B) FseI digestion of genomic DNA and hybridization with dig-labeled senX3 probe reveals fragments measuring 3.7-kb and 5.7-kb in CDC1551 and senX 3::Tn, respectively, as well as 5.7-kb and 7.7-kb fragments in senX3 ::Tn Comp. (C) SphI digestion of genomic DNA followed by hybridization with dig-labeled regX3 probe reveals 5.5-kb and 7.5-kb fragments in CDC1551 and regX3 ::Tn, respectively, as well as 7.5-kb and 4.6-kb fragments in regX3 ::Tn Comp. (D) Southern analysis of senX3 complementation. Lanes 1 and 2: SenX3 ::Tn Comp candidates with appropriately-sized bands; Lane 3: SenX 3::Tn; Lane 4: Wild-type CDC1551. (E) Southern analysis of regX3 complementation. Lane 1: Wild-type CDC551; Lane 2: regX 3::Tn; Lanes 3–5: regX3 ::Tn Comp candidates with appropriately-sized bands.

Article Snippet: Genomic DNA from senX3 ::Tn and regX3 ::Tn complement candidates was digested with FseI and SphI-HF (New England Biolabs), respectively, and electrophoresis was performed on 1% agarose gels.

Techniques: Hybridization, Labeling

Human Exo1-biotin (hExo1-bio) purification and labeling. (A) Purification scheme for hExo1-bio. (B) SDS/PAGE gel showing hExo1-bio and hExo1-bio + streptavidin. Gel shift of hExo1-bio-streptavidin conjugates are indicated. The complete disappearance of the hExo1-bio band indicates that nearly 100% of the purified nucleases are biotinylated. (C) Resection assay with untagged WT hExo1 (500 pM), hExo1-bio (500 pM), or hExo1-bio (500 pM) + streptavidin (1 µg). Proteins were incubated in imaging buffer with 30 ng linearized 4.4-kb DNA (4-nt 3′ overhang) for 30 min at 37 °C. Resected DNA was separated on a 1% agarose gel and stained with SYBR green. The resection protocol has also been described previously (21). Together, these assays indicate that streptavidin-conjugated hExo1-bio retains full resection activity. (D) Snapshots at indicated times (Upper) and single-particle tracking of two representative trajectories of resection by CF488-anti-biotin–labeled hExo1 (Lower). In both trajectories, hExo1 transitions between a resecting and a paused state. These results indicate that both states are intrinsic to hExo1 and are not dependent on the nature of the fluorophore.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Single-molecule imaging reveals the mechanism of Exo1 regulation by single-stranded DNA binding proteins

doi: 10.1073/pnas.1516674113

Figure Lengend Snippet: Human Exo1-biotin (hExo1-bio) purification and labeling. (A) Purification scheme for hExo1-bio. (B) SDS/PAGE gel showing hExo1-bio and hExo1-bio + streptavidin. Gel shift of hExo1-bio-streptavidin conjugates are indicated. The complete disappearance of the hExo1-bio band indicates that nearly 100% of the purified nucleases are biotinylated. (C) Resection assay with untagged WT hExo1 (500 pM), hExo1-bio (500 pM), or hExo1-bio (500 pM) + streptavidin (1 µg). Proteins were incubated in imaging buffer with 30 ng linearized 4.4-kb DNA (4-nt 3′ overhang) for 30 min at 37 °C. Resected DNA was separated on a 1% agarose gel and stained with SYBR green. The resection protocol has also been described previously (21). Together, these assays indicate that streptavidin-conjugated hExo1-bio retains full resection activity. (D) Snapshots at indicated times (Upper) and single-particle tracking of two representative trajectories of resection by CF488-anti-biotin–labeled hExo1 (Lower). In both trajectories, hExo1 transitions between a resecting and a paused state. These results indicate that both states are intrinsic to hExo1 and are not dependent on the nature of the fluorophore.

Article Snippet: Briefly, WT hExo1 (500 pM), hExo1-bio (500 pM), or hExo1-bio (500 pM) + streptavidin (1 µg) was incubated in imaging buffer with 30 ng linearized 4.4-kb DNA (4-nt 3′ overhang created with SphI-HF; NEB) for 30 min at 37 °C.

Techniques: Purification, Labeling, SDS Page, Electrophoretic Mobility Shift Assay, Resection Assay, Incubation, Imaging, Agarose Gel Electrophoresis, Staining, SYBR Green Assay, Activity Assay, Single-particle Tracking