R31385 Search Results


chaperone constructs  (New England Biolabs)
96
New England Biolabs chaperone constructs
Chaperone Constructs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restriction enzymes  (New England Biolabs)
96
New England Biolabs restriction enzymes
Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sali hf  (New England Biolabs)
96
New England Biolabs sali hf
Sali Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sali restriction enzymes  (New England Biolabs)
99
New England Biolabs sali restriction enzymes
Sali Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vector 1180  (New England Biolabs)
97
New England Biolabs vector 1180
BmPGRP2 expression patterns. (A) Schematic representation of BmPGRP2 gene structure from DZ silkworm. Two forms of BmPGRP2, BmPGRP2-1 , and -2 , were cloned. The 5′ UTR was amplified with primers 5F-1,-2,-3,-4,-5, and-6 and 5R-1 and-2. The 5′ UTR of BmPGRP2-1 was longer and contained that of BmPGRP2-2 . (B) Analysis of BmPGRP2 expression at whole individual of different developmental stages by RT-PCR. Points 1 to 4 represent 2-, 4-, 6-, and 8-day-old eggs; 5 to 13 represent hatched silkworm, first instar molt, second instar, second instar molt, third instar, third instar molt, fourth instar, fourth instar molt, and fifth instar larvae, respectively; 14 to 17 represent 2-, 4-, 6-, and 8-day-old pupae, respectively; and 18 represents moth. TIF-4A (GenBank: DQ443290.1 ) was the internal control. 25C and 30C represented the PCR amplification cycles was 25 and 30, respectively. (C) qPCR analysis of BmPGRP2 expression in the head, cuticle, hemocyte, midgut, fat body, silk gland, trachea, malpighian, ovary, and testis of female and male silkworms. TIF-4A was used as the internal control. Bars represent standard deviation. (D) Subcellular localization of BmPGRP2. The vector 1180-A4P-GFP- BmPGRP2-1/2 -SV40 and 1180-A4P-GFP-SV40 (A4-GFP) was transfected into BmE cells, respectively. The nucleus was dye blue by DAPI, and green fluorescence represented the location of target protein.
Vector 1180, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vector 1180 - by Bioz Stars, 2026-02
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bamhi hf  (New England Biolabs)
99
New England Biolabs bamhi hf
BmPGRP2 expression patterns. (A) Schematic representation of BmPGRP2 gene structure from DZ silkworm. Two forms of BmPGRP2, BmPGRP2-1 , and -2 , were cloned. The 5′ UTR was amplified with primers 5F-1,-2,-3,-4,-5, and-6 and 5R-1 and-2. The 5′ UTR of BmPGRP2-1 was longer and contained that of BmPGRP2-2 . (B) Analysis of BmPGRP2 expression at whole individual of different developmental stages by RT-PCR. Points 1 to 4 represent 2-, 4-, 6-, and 8-day-old eggs; 5 to 13 represent hatched silkworm, first instar molt, second instar, second instar molt, third instar, third instar molt, fourth instar, fourth instar molt, and fifth instar larvae, respectively; 14 to 17 represent 2-, 4-, 6-, and 8-day-old pupae, respectively; and 18 represents moth. TIF-4A (GenBank: DQ443290.1 ) was the internal control. 25C and 30C represented the PCR amplification cycles was 25 and 30, respectively. (C) qPCR analysis of BmPGRP2 expression in the head, cuticle, hemocyte, midgut, fat body, silk gland, trachea, malpighian, ovary, and testis of female and male silkworms. TIF-4A was used as the internal control. Bars represent standard deviation. (D) Subcellular localization of BmPGRP2. The vector 1180-A4P-GFP- BmPGRP2-1/2 -SV40 and 1180-A4P-GFP-SV40 (A4-GFP) was transfected into BmE cells, respectively. The nucleus was dye blue by DAPI, and green fluorescence represented the location of target protein.
Bamhi Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t4 dna ligase neb  (New England Biolabs)
97
New England Biolabs t4 dna ligase neb
BmPGRP2 expression patterns. (A) Schematic representation of BmPGRP2 gene structure from DZ silkworm. Two forms of BmPGRP2, BmPGRP2-1 , and -2 , were cloned. The 5′ UTR was amplified with primers 5F-1,-2,-3,-4,-5, and-6 and 5R-1 and-2. The 5′ UTR of BmPGRP2-1 was longer and contained that of BmPGRP2-2 . (B) Analysis of BmPGRP2 expression at whole individual of different developmental stages by RT-PCR. Points 1 to 4 represent 2-, 4-, 6-, and 8-day-old eggs; 5 to 13 represent hatched silkworm, first instar molt, second instar, second instar molt, third instar, third instar molt, fourth instar, fourth instar molt, and fifth instar larvae, respectively; 14 to 17 represent 2-, 4-, 6-, and 8-day-old pupae, respectively; and 18 represents moth. TIF-4A (GenBank: DQ443290.1 ) was the internal control. 25C and 30C represented the PCR amplification cycles was 25 and 30, respectively. (C) qPCR analysis of BmPGRP2 expression in the head, cuticle, hemocyte, midgut, fat body, silk gland, trachea, malpighian, ovary, and testis of female and male silkworms. TIF-4A was used as the internal control. Bars represent standard deviation. (D) Subcellular localization of BmPGRP2. The vector 1180-A4P-GFP- BmPGRP2-1/2 -SV40 and 1180-A4P-GFP-SV40 (A4-GFP) was transfected into BmE cells, respectively. The nucleus was dye blue by DAPI, and green fluorescence represented the location of target protein.
T4 Dna Ligase Neb, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rcutsmart buffer  (New England Biolabs)
96
New England Biolabs rcutsmart buffer
BmPGRP2 expression patterns. (A) Schematic representation of BmPGRP2 gene structure from DZ silkworm. Two forms of BmPGRP2, BmPGRP2-1 , and -2 , were cloned. The 5′ UTR was amplified with primers 5F-1,-2,-3,-4,-5, and-6 and 5R-1 and-2. The 5′ UTR of BmPGRP2-1 was longer and contained that of BmPGRP2-2 . (B) Analysis of BmPGRP2 expression at whole individual of different developmental stages by RT-PCR. Points 1 to 4 represent 2-, 4-, 6-, and 8-day-old eggs; 5 to 13 represent hatched silkworm, first instar molt, second instar, second instar molt, third instar, third instar molt, fourth instar, fourth instar molt, and fifth instar larvae, respectively; 14 to 17 represent 2-, 4-, 6-, and 8-day-old pupae, respectively; and 18 represents moth. TIF-4A (GenBank: DQ443290.1 ) was the internal control. 25C and 30C represented the PCR amplification cycles was 25 and 30, respectively. (C) qPCR analysis of BmPGRP2 expression in the head, cuticle, hemocyte, midgut, fat body, silk gland, trachea, malpighian, ovary, and testis of female and male silkworms. TIF-4A was used as the internal control. Bars represent standard deviation. (D) Subcellular localization of BmPGRP2. The vector 1180-A4P-GFP- BmPGRP2-1/2 -SV40 and 1180-A4P-GFP-SV40 (A4-GFP) was transfected into BmE cells, respectively. The nucleus was dye blue by DAPI, and green fluorescence represented the location of target protein.
Rcutsmart Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dpni  (New England Biolabs)
96
New England Biolabs dpni
BmPGRP2 expression patterns. (A) Schematic representation of BmPGRP2 gene structure from DZ silkworm. Two forms of BmPGRP2, BmPGRP2-1 , and -2 , were cloned. The 5′ UTR was amplified with primers 5F-1,-2,-3,-4,-5, and-6 and 5R-1 and-2. The 5′ UTR of BmPGRP2-1 was longer and contained that of BmPGRP2-2 . (B) Analysis of BmPGRP2 expression at whole individual of different developmental stages by RT-PCR. Points 1 to 4 represent 2-, 4-, 6-, and 8-day-old eggs; 5 to 13 represent hatched silkworm, first instar molt, second instar, second instar molt, third instar, third instar molt, fourth instar, fourth instar molt, and fifth instar larvae, respectively; 14 to 17 represent 2-, 4-, 6-, and 8-day-old pupae, respectively; and 18 represents moth. TIF-4A (GenBank: DQ443290.1 ) was the internal control. 25C and 30C represented the PCR amplification cycles was 25 and 30, respectively. (C) qPCR analysis of BmPGRP2 expression in the head, cuticle, hemocyte, midgut, fat body, silk gland, trachea, malpighian, ovary, and testis of female and male silkworms. TIF-4A was used as the internal control. Bars represent standard deviation. (D) Subcellular localization of BmPGRP2. The vector 1180-A4P-GFP- BmPGRP2-1/2 -SV40 and 1180-A4P-GFP-SV40 (A4-GFP) was transfected into BmE cells, respectively. The nucleus was dye blue by DAPI, and green fluorescence represented the location of target protein.
Dpni, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dpni neb cat  (New England Biolabs)
96
New England Biolabs dpni neb cat
BmPGRP2 expression patterns. (A) Schematic representation of BmPGRP2 gene structure from DZ silkworm. Two forms of BmPGRP2, BmPGRP2-1 , and -2 , were cloned. The 5′ UTR was amplified with primers 5F-1,-2,-3,-4,-5, and-6 and 5R-1 and-2. The 5′ UTR of BmPGRP2-1 was longer and contained that of BmPGRP2-2 . (B) Analysis of BmPGRP2 expression at whole individual of different developmental stages by RT-PCR. Points 1 to 4 represent 2-, 4-, 6-, and 8-day-old eggs; 5 to 13 represent hatched silkworm, first instar molt, second instar, second instar molt, third instar, third instar molt, fourth instar, fourth instar molt, and fifth instar larvae, respectively; 14 to 17 represent 2-, 4-, 6-, and 8-day-old pupae, respectively; and 18 represents moth. TIF-4A (GenBank: DQ443290.1 ) was the internal control. 25C and 30C represented the PCR amplification cycles was 25 and 30, respectively. (C) qPCR analysis of BmPGRP2 expression in the head, cuticle, hemocyte, midgut, fat body, silk gland, trachea, malpighian, ovary, and testis of female and male silkworms. TIF-4A was used as the internal control. Bars represent standard deviation. (D) Subcellular localization of BmPGRP2. The vector 1180-A4P-GFP- BmPGRP2-1/2 -SV40 and 1180-A4P-GFP-SV40 (A4-GFP) was transfected into BmE cells, respectively. The nucleus was dye blue by DAPI, and green fluorescence represented the location of target protein.
Dpni Neb Cat, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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casein biorad 1610783 mlui neb r0198s sali hf neb r3138s t4 ligase neb m0202s critical  (Bio-Rad)
96
Bio-Rad casein biorad 1610783 mlui neb r0198s sali hf neb r3138s t4 ligase neb m0202s critical
BmPGRP2 expression patterns. (A) Schematic representation of BmPGRP2 gene structure from DZ silkworm. Two forms of BmPGRP2, BmPGRP2-1 , and -2 , were cloned. The 5′ UTR was amplified with primers 5F-1,-2,-3,-4,-5, and-6 and 5R-1 and-2. The 5′ UTR of BmPGRP2-1 was longer and contained that of BmPGRP2-2 . (B) Analysis of BmPGRP2 expression at whole individual of different developmental stages by RT-PCR. Points 1 to 4 represent 2-, 4-, 6-, and 8-day-old eggs; 5 to 13 represent hatched silkworm, first instar molt, second instar, second instar molt, third instar, third instar molt, fourth instar, fourth instar molt, and fifth instar larvae, respectively; 14 to 17 represent 2-, 4-, 6-, and 8-day-old pupae, respectively; and 18 represents moth. TIF-4A (GenBank: DQ443290.1 ) was the internal control. 25C and 30C represented the PCR amplification cycles was 25 and 30, respectively. (C) qPCR analysis of BmPGRP2 expression in the head, cuticle, hemocyte, midgut, fat body, silk gland, trachea, malpighian, ovary, and testis of female and male silkworms. TIF-4A was used as the internal control. Bars represent standard deviation. (D) Subcellular localization of BmPGRP2. The vector 1180-A4P-GFP- BmPGRP2-1/2 -SV40 and 1180-A4P-GFP-SV40 (A4-GFP) was transfected into BmE cells, respectively. The nucleus was dye blue by DAPI, and green fluorescence represented the location of target protein.
Casein Biorad 1610783 Mlui Neb R0198s Sali Hf Neb R3138s T4 Ligase Neb M0202s Critical, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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casein biorad 1610783 mlui neb r0198s sali hf neb r3138s t4 ligase neb m0202s critical - by Bioz Stars, 2026-02
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noti sali  (New England Biolabs)
96
New England Biolabs noti sali
BmPGRP2 expression patterns. (A) Schematic representation of BmPGRP2 gene structure from DZ silkworm. Two forms of BmPGRP2, BmPGRP2-1 , and -2 , were cloned. The 5′ UTR was amplified with primers 5F-1,-2,-3,-4,-5, and-6 and 5R-1 and-2. The 5′ UTR of BmPGRP2-1 was longer and contained that of BmPGRP2-2 . (B) Analysis of BmPGRP2 expression at whole individual of different developmental stages by RT-PCR. Points 1 to 4 represent 2-, 4-, 6-, and 8-day-old eggs; 5 to 13 represent hatched silkworm, first instar molt, second instar, second instar molt, third instar, third instar molt, fourth instar, fourth instar molt, and fifth instar larvae, respectively; 14 to 17 represent 2-, 4-, 6-, and 8-day-old pupae, respectively; and 18 represents moth. TIF-4A (GenBank: DQ443290.1 ) was the internal control. 25C and 30C represented the PCR amplification cycles was 25 and 30, respectively. (C) qPCR analysis of BmPGRP2 expression in the head, cuticle, hemocyte, midgut, fat body, silk gland, trachea, malpighian, ovary, and testis of female and male silkworms. TIF-4A was used as the internal control. Bars represent standard deviation. (D) Subcellular localization of BmPGRP2. The vector 1180-A4P-GFP- BmPGRP2-1/2 -SV40 and 1180-A4P-GFP-SV40 (A4-GFP) was transfected into BmE cells, respectively. The nucleus was dye blue by DAPI, and green fluorescence represented the location of target protein.
Noti Sali, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


BmPGRP2 expression patterns. (A) Schematic representation of BmPGRP2 gene structure from DZ silkworm. Two forms of BmPGRP2, BmPGRP2-1 , and -2 , were cloned. The 5′ UTR was amplified with primers 5F-1,-2,-3,-4,-5, and-6 and 5R-1 and-2. The 5′ UTR of BmPGRP2-1 was longer and contained that of BmPGRP2-2 . (B) Analysis of BmPGRP2 expression at whole individual of different developmental stages by RT-PCR. Points 1 to 4 represent 2-, 4-, 6-, and 8-day-old eggs; 5 to 13 represent hatched silkworm, first instar molt, second instar, second instar molt, third instar, third instar molt, fourth instar, fourth instar molt, and fifth instar larvae, respectively; 14 to 17 represent 2-, 4-, 6-, and 8-day-old pupae, respectively; and 18 represents moth. TIF-4A (GenBank: DQ443290.1 ) was the internal control. 25C and 30C represented the PCR amplification cycles was 25 and 30, respectively. (C) qPCR analysis of BmPGRP2 expression in the head, cuticle, hemocyte, midgut, fat body, silk gland, trachea, malpighian, ovary, and testis of female and male silkworms. TIF-4A was used as the internal control. Bars represent standard deviation. (D) Subcellular localization of BmPGRP2. The vector 1180-A4P-GFP- BmPGRP2-1/2 -SV40 and 1180-A4P-GFP-SV40 (A4-GFP) was transfected into BmE cells, respectively. The nucleus was dye blue by DAPI, and green fluorescence represented the location of target protein.

Journal: Frontiers in Immunology

Article Title: Distinct Functions of Bombyx mori Peptidoglycan Recognition Protein 2 in Immune Responses to Bacteria and Viruses

doi: 10.3389/fimmu.2019.00776

Figure Lengend Snippet: BmPGRP2 expression patterns. (A) Schematic representation of BmPGRP2 gene structure from DZ silkworm. Two forms of BmPGRP2, BmPGRP2-1 , and -2 , were cloned. The 5′ UTR was amplified with primers 5F-1,-2,-3,-4,-5, and-6 and 5R-1 and-2. The 5′ UTR of BmPGRP2-1 was longer and contained that of BmPGRP2-2 . (B) Analysis of BmPGRP2 expression at whole individual of different developmental stages by RT-PCR. Points 1 to 4 represent 2-, 4-, 6-, and 8-day-old eggs; 5 to 13 represent hatched silkworm, first instar molt, second instar, second instar molt, third instar, third instar molt, fourth instar, fourth instar molt, and fifth instar larvae, respectively; 14 to 17 represent 2-, 4-, 6-, and 8-day-old pupae, respectively; and 18 represents moth. TIF-4A (GenBank: DQ443290.1 ) was the internal control. 25C and 30C represented the PCR amplification cycles was 25 and 30, respectively. (C) qPCR analysis of BmPGRP2 expression in the head, cuticle, hemocyte, midgut, fat body, silk gland, trachea, malpighian, ovary, and testis of female and male silkworms. TIF-4A was used as the internal control. Bars represent standard deviation. (D) Subcellular localization of BmPGRP2. The vector 1180-A4P-GFP- BmPGRP2-1/2 -SV40 and 1180-A4P-GFP-SV40 (A4-GFP) was transfected into BmE cells, respectively. The nucleus was dye blue by DAPI, and green fluorescence represented the location of target protein.

Article Snippet: A synthetic sequence includes B. mori A4 promoter (A4P) and GFP was cloned into the empty vector 1180 (GenBank: U13865.1 ) using SalI (#R3138V, NEB, USA) and BamHI (#R3136V, NEB, USA) restriction enzymes, followed adding BmPGRP2-1 or -2 [using BamHI and NotI (#R3189V, NEB, USA)] and Simian virus (SV)40 [using NotI and HindIII (#R3104V, NEB, USA)] to construct 1180-A4P-GFP- BmPGRP2-1 /2-SV40, which was transfected into BmE cells for subcellular localization analysis.

Techniques: Expressing, Clone Assay, Amplification, Reverse Transcription Polymerase Chain Reaction, Standard Deviation, Plasmid Preparation, Transfection, Fluorescence

BmPGRP2-2 negatively regulates PTEN . (A) qPCR analysis of PTEN expression (using control TIF-4A ) in PGRP2-2I. PGRP2-2I and 932 were orally infected with BmNPV on day 3 of the 5th instar. RNA was extracted from the midgut (MG) and fat body (Fb) at 3, 6, 12, and 24 hpi. (B) Detection of PTEN (using control TIF-4A ) after BmPGRP2-2 RNAi and overexpression. (C) qPCR analysis of BmPGRP2-2 (using control TIF-4A ) after PTEN overexpression (D) Subcellular localization of PTEN. The vector 1180-A4P-GFP- PTEN -SV40 was transfected into BmE cells. The green fluorescence represented the location of target protein and blue indicated nucleus. Bars, standard deviations. Significant differences, ** P < 0.01, * P < 0.05.

Journal: Frontiers in Immunology

Article Title: Distinct Functions of Bombyx mori Peptidoglycan Recognition Protein 2 in Immune Responses to Bacteria and Viruses

doi: 10.3389/fimmu.2019.00776

Figure Lengend Snippet: BmPGRP2-2 negatively regulates PTEN . (A) qPCR analysis of PTEN expression (using control TIF-4A ) in PGRP2-2I. PGRP2-2I and 932 were orally infected with BmNPV on day 3 of the 5th instar. RNA was extracted from the midgut (MG) and fat body (Fb) at 3, 6, 12, and 24 hpi. (B) Detection of PTEN (using control TIF-4A ) after BmPGRP2-2 RNAi and overexpression. (C) qPCR analysis of BmPGRP2-2 (using control TIF-4A ) after PTEN overexpression (D) Subcellular localization of PTEN. The vector 1180-A4P-GFP- PTEN -SV40 was transfected into BmE cells. The green fluorescence represented the location of target protein and blue indicated nucleus. Bars, standard deviations. Significant differences, ** P < 0.01, * P < 0.05.

Article Snippet: A synthetic sequence includes B. mori A4 promoter (A4P) and GFP was cloned into the empty vector 1180 (GenBank: U13865.1 ) using SalI (#R3138V, NEB, USA) and BamHI (#R3136V, NEB, USA) restriction enzymes, followed adding BmPGRP2-1 or -2 [using BamHI and NotI (#R3189V, NEB, USA)] and Simian virus (SV)40 [using NotI and HindIII (#R3104V, NEB, USA)] to construct 1180-A4P-GFP- BmPGRP2-1 /2-SV40, which was transfected into BmE cells for subcellular localization analysis.

Techniques: Expressing, Infection, Over Expression, Plasmid Preparation, Transfection, Fluorescence