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    New England Biolabs hinp1i
    Digestion of the single-site plasmid pC194 with BspRI, <t>HinP1I</t> and BsuRI endonucleases. ( A ) Agarose gel electrophoresis of the digestion products. Reaction times are shown above the lanes. Digestions were performed as described in ‘Materials and Methods’ section. Sc, l and oc denote supercoiled, linear and open circular forms, respectively. ( B ) Proposed kinetic scheme for DNA cleavage by BspRI endonuclease. Step 1, equilibrium binding to uncut DNA; step 2, nicking of the first strand; step 3, equilibrium binding to nicked DNA, BspRI facing the nicked strand; step 4, equilibrium binding to nicked DNA, BspRI facing the intact strand; step 5, transposition of BspRI from the nicked strand to the uncut strand; step 6, cleaving the second strand. ( C ) Time course of digestion of supercoiled plasmid DNA with BspRI. pC194 plasmid DNA was digested for the indicated times and the plasmid forms were quantitated as described in ‘Materials and Methods’ section. Experimental data are denoted by symbols (circle, supercoiled; diamond, nicked; square, linear). Lines represent results of the simulation (dashed–dotted, supercoiled; solid, nicked; dashed, linear). Error bars indicate the standard error of the data calculated from four parallel experiments.
    Hinp1i, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Digestion of the single-site plasmid pC194 with BspRI, HinP1I and BsuRI endonucleases. ( A ) Agarose gel electrophoresis of the digestion products. Reaction times are shown above the lanes. Digestions were performed as described in ‘Materials and Methods’ section. Sc, l and oc denote supercoiled, linear and open circular forms, respectively. ( B ) Proposed kinetic scheme for DNA cleavage by BspRI endonuclease. Step 1, equilibrium binding to uncut DNA; step 2, nicking of the first strand; step 3, equilibrium binding to nicked DNA, BspRI facing the nicked strand; step 4, equilibrium binding to nicked DNA, BspRI facing the intact strand; step 5, transposition of BspRI from the nicked strand to the uncut strand; step 6, cleaving the second strand. ( C ) Time course of digestion of supercoiled plasmid DNA with BspRI. pC194 plasmid DNA was digested for the indicated times and the plasmid forms were quantitated as described in ‘Materials and Methods’ section. Experimental data are denoted by symbols (circle, supercoiled; diamond, nicked; square, linear). Lines represent results of the simulation (dashed–dotted, supercoiled; solid, nicked; dashed, linear). Error bars indicate the standard error of the data calculated from four parallel experiments.

    Journal: Nucleic Acids Research

    Article Title: BspRI restriction endonuclease: cloning, expression in Escherichia coli and sequential cleavage mechanism

    doi: 10.1093/nar/gkq567

    Figure Lengend Snippet: Digestion of the single-site plasmid pC194 with BspRI, HinP1I and BsuRI endonucleases. ( A ) Agarose gel electrophoresis of the digestion products. Reaction times are shown above the lanes. Digestions were performed as described in ‘Materials and Methods’ section. Sc, l and oc denote supercoiled, linear and open circular forms, respectively. ( B ) Proposed kinetic scheme for DNA cleavage by BspRI endonuclease. Step 1, equilibrium binding to uncut DNA; step 2, nicking of the first strand; step 3, equilibrium binding to nicked DNA, BspRI facing the nicked strand; step 4, equilibrium binding to nicked DNA, BspRI facing the intact strand; step 5, transposition of BspRI from the nicked strand to the uncut strand; step 6, cleaving the second strand. ( C ) Time course of digestion of supercoiled plasmid DNA with BspRI. pC194 plasmid DNA was digested for the indicated times and the plasmid forms were quantitated as described in ‘Materials and Methods’ section. Experimental data are denoted by symbols (circle, supercoiled; diamond, nicked; square, linear). Lines represent results of the simulation (dashed–dotted, supercoiled; solid, nicked; dashed, linear). Error bars indicate the standard error of the data calculated from four parallel experiments.

    Article Snippet: Conversion of supercoiled pC194 into the linear form by HinP1I was accompanied by the appearance of the open circular intermediate in a very similar fashion as for BspRI ( A), which is consistent with both enzymes acting as a monomer.

    Techniques: Plasmid Preparation, Agarose Gel Electrophoresis, Binding Assay

    RFLP analysis of 16S rDNA clones from the EPSs from four patients. Agarose gel electrophoresis of partial 16S rDNA digested with Msp I and Hin P1I from one 96-well plate was performed. The RFLP types were counted, and unique banding patterns were analyzed further by sequencing.

    Journal: Journal of Clinical Microbiology

    Article Title: Prevalence of Corynebacterial 16S rRNA Sequences in Patients with Bacterial and "Nonbacterial" Prostatitis

    doi:

    Figure Lengend Snippet: RFLP analysis of 16S rDNA clones from the EPSs from four patients. Agarose gel electrophoresis of partial 16S rDNA digested with Msp I and Hin P1I from one 96-well plate was performed. The RFLP types were counted, and unique banding patterns were analyzed further by sequencing.

    Article Snippet: For RFLP analysis, DNA was digested with Msp I and Hin P1I in NEBuffer 2 (New England Biolabs, Beverly, Mass.) and 0.01% Triton X-100.

    Techniques: Clone Assay, Agarose Gel Electrophoresis, Sequencing

    Enhanced amplification of viral fragments using one restriction enzyme in VIDISCA. Visualization of VIDISCA fragments digested with HinP1-I+MseI or MseI alone. VIDISCA fragments are visualized on a 1% agarose gel, which were generated after a single first round PCR of 40 cycles. The dots indicate viral fragments which were only visible with MseI digestion.

    Journal: PLoS ONE

    Article Title: A Sensitive Assay for Virus Discovery in Respiratory Clinical Samples

    doi: 10.1371/journal.pone.0016118

    Figure Lengend Snippet: Enhanced amplification of viral fragments using one restriction enzyme in VIDISCA. Visualization of VIDISCA fragments digested with HinP1-I+MseI or MseI alone. VIDISCA fragments are visualized on a 1% agarose gel, which were generated after a single first round PCR of 40 cycles. The dots indicate viral fragments which were only visible with MseI digestion.

    Article Snippet: The digestion was performed for 2h at 37°C by 10 U of HinP1-I (New England Biolabs) and 10U of MseI (New England Biolabs) restriction enzymes or only by 10U of MseI (New England Biolabs).

    Techniques: Amplification, Agarose Gel Electrophoresis, Generated, Polymerase Chain Reaction