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    New England Biolabs apai
    Pulse-field gel electrophoresis patterns of ( a ) <t>XbaI-digested</t> genomic DNA of B. breve isolates and ( b ) <t>ApaI-digested</t> genomic DNA of L. plantarum isolates from human milk and infant faeces. The unedited versions of these images can be found as Supplementary Figures S3 and S4 .
    Apai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1193 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pulse-field gel electrophoresis patterns of ( a ) XbaI-digested genomic DNA of B. breve isolates and ( b ) ApaI-digested genomic DNA of L. plantarum isolates from human milk and infant faeces. The unedited versions of these images can be found as Supplementary Figures S3 and S4 .

    Journal: Scientific Reports

    Article Title: The Composition of Human Milk and Infant Faecal Microbiota Over the First Three Months of Life: A Pilot Study

    doi: 10.1038/srep40597

    Figure Lengend Snippet: Pulse-field gel electrophoresis patterns of ( a ) XbaI-digested genomic DNA of B. breve isolates and ( b ) ApaI-digested genomic DNA of L. plantarum isolates from human milk and infant faeces. The unedited versions of these images can be found as Supplementary Figures S3 and S4 .

    Article Snippet: The restriction enzyme XbaI was used to cleave bifidobacterial chromosomal DNA and ApaI (New England Biolabs, MA, United States) was used for Lactobacillus .

    Techniques: Nucleic Acid Electrophoresis

    a - d Restriction endonuclease digestion for FokI, ApaI, Bsm I and TaqI polymorphisms, M = Marker

    Journal: BMC Nephrology

    Article Title: Influence of vitamin D receptor polymorphisms on biochemical markers of mineral bone disorders in South African patients with chronic kidney disease

    doi: 10.1186/s12882-018-0831-7

    Figure Lengend Snippet: a - d Restriction endonuclease digestion for FokI, ApaI, Bsm I and TaqI polymorphisms, M = Marker

    Article Snippet: The PCR products were then digested with enzymes ApaI, BsmI, FokI, and TaqI (New England Biolabs, Beverly, MA, USA) according to the supplier’s protocol.

    Techniques: Marker

    Frequency of vitamin D deficiency according to BsmI (A), ApaI (B), and TaqI (C) SNPs in VDR gene grouped by presence of polymorphic allele. White bars express the frequency of vitamin D status ( deficiency or insufficiency/sufficiency) for the wild genotype of each VDR SNP. Gray bars express the frequency of vitamin D status for the polymorphic (in heterozygous plus homozygous) genotypes. Pearson chi-square test, * p

    Journal: BMC Pediatrics

    Article Title: Vitamin D deficiency in girls from South Brazil: a cross-sectional study on prevalence and association with vitamin D receptor gene variants

    doi: 10.1186/1471-2431-12-62

    Figure Lengend Snippet: Frequency of vitamin D deficiency according to BsmI (A), ApaI (B), and TaqI (C) SNPs in VDR gene grouped by presence of polymorphic allele. White bars express the frequency of vitamin D status ( deficiency or insufficiency/sufficiency) for the wild genotype of each VDR SNP. Gray bars express the frequency of vitamin D status for the polymorphic (in heterozygous plus homozygous) genotypes. Pearson chi-square test, * p

    Article Snippet: Protocol conditions consisted of denaturation at 95°C for 2 min followed by 35 cycles (95°C, 30sec; 59.2°C, 30sec; 72°C, 80sec) and final extension at 72°C for 5 min. PCR products were digested overnight by the restriction enzymes ApaI or TaqI (New England Biolabs, USA) at 37°C or 65°C, respectively.

    Techniques:

    Vitamin D levels according to BsmI (A), ApaI (B), and TaqI (C) SNPs in VDR gene grouped by presence of polymorphic allele. Values are expressed as means (central circle) and 95% CI (lower and upper limit). Two-tailed Student’s t -test, * p

    Journal: BMC Pediatrics

    Article Title: Vitamin D deficiency in girls from South Brazil: a cross-sectional study on prevalence and association with vitamin D receptor gene variants

    doi: 10.1186/1471-2431-12-62

    Figure Lengend Snippet: Vitamin D levels according to BsmI (A), ApaI (B), and TaqI (C) SNPs in VDR gene grouped by presence of polymorphic allele. Values are expressed as means (central circle) and 95% CI (lower and upper limit). Two-tailed Student’s t -test, * p

    Article Snippet: Protocol conditions consisted of denaturation at 95°C for 2 min followed by 35 cycles (95°C, 30sec; 59.2°C, 30sec; 72°C, 80sec) and final extension at 72°C for 5 min. PCR products were digested overnight by the restriction enzymes ApaI or TaqI (New England Biolabs, USA) at 37°C or 65°C, respectively.

    Techniques: Two Tailed Test

    Dendrogram produced by the unweighted-pair group method using average linkages showing clustering by Dice coefficient of the combined PFGE (AscI and ApaI) profiles of the 20 L. monocytogenes isolates obtained from animal cases analyzed in this study.

    Journal: Applied and Environmental Microbiology

    Article Title: Ruminant Rhombencephalitis-Associated Listeria monocytogenes Strains Constitute a Genetically Homogeneous Group Related to Human Outbreak Strains

    doi: 10.1128/AEM.00219-13

    Figure Lengend Snippet: Dendrogram produced by the unweighted-pair group method using average linkages showing clustering by Dice coefficient of the combined PFGE (AscI and ApaI) profiles of the 20 L. monocytogenes isolates obtained from animal cases analyzed in this study.

    Article Snippet: PFGE was performed by including genomic DNA in agarose plugs prior to digestion with the AscI and ApaI restriction enzymes (New England BioLabs), followed by PFGE with a Chef DR III system (Bio-Rad, Hercules, CA) , and pulsotypes were analyzed as described elsewhere ( ).

    Techniques: Produced

    a Location of TaqMan PCR Cy5 probe in the lambda genome is shown in red , FAM probes are shown in green , and the location of the ApaI restriction site is shown in black . b Representative image of multiplexed PCR emulsion on Lambda DNA. c Representative scatterplot of Cy5 and FAM intensities for Lambda DNA. d Fraction of multiplexed droplets for Lambda DNA undigested ( blue ), ApaI digested ( red ), and Fragmentase digested ( green )

    Journal: Virology Journal

    Article Title: Peering below the diffraction limit: robust and specific sorting of viruses with flow cytometry

    doi: 10.1186/s12985-016-0655-7

    Figure Lengend Snippet: a Location of TaqMan PCR Cy5 probe in the lambda genome is shown in red , FAM probes are shown in green , and the location of the ApaI restriction site is shown in black . b Representative image of multiplexed PCR emulsion on Lambda DNA. c Representative scatterplot of Cy5 and FAM intensities for Lambda DNA. d Fraction of multiplexed droplets for Lambda DNA undigested ( blue ), ApaI digested ( red ), and Fragmentase digested ( green )

    Article Snippet: For ApaI restriction enzyme digestion (NEB) or Fragmentase digest (New England Biolabs; NEB), Lambda DNA is treated enzymatically prior to the mixing with the PCR reagents.

    Techniques: Polymerase Chain Reaction, Lambda DNA Preparation