Journal: Investigative Ophthalmology & Visual Science

Article Title: Compositional Analysis of Extracellular Aggregates in the Eyes of Patients With Exfoliation Syndrome and Exfoliation Glaucoma

doi: 10.1167/iovs.62.15.27

Figure Lengend Snippet: Primary Antibodies Used in Immunofluorescence Studies of XFM on Lens Capsule Samples

Article Snippet: LOXL1 catalytic domain , Polyclonal , 1:100 , PB9719 , Boster, Pleasanton, CA.

Techniques: Immunofluorescence

Journal: Investigative Ophthalmology & Visual Science

Article Title: Compositional Analysis of Extracellular Aggregates in the Eyes of Patients With Exfoliation Syndrome and Exfoliation Glaucoma

doi: 10.1167/iovs.62.15.27

Figure Lengend Snippet: Relative abundance of three putative XFM components (FBN1, lysyl oxidase-like 1 (LOXL1), and LEFTY2, in human capsule specimens. Specimens were obtained from cataract patients (CAT), cataract patients with glaucoma (CAT/GL), cataract patients with XFS, and cataract patients with XFG. Bars represent the fraction of total protein present, as estimated from reporter ion intensities for that protein in each sample relative to the total reporter ion intensity for all proteins.

Article Snippet: LOXL1 catalytic domain , Polyclonal , 1:100 , PB9719 , Boster, Pleasanton, CA.

Techniques:

Journal: Investigative Ophthalmology & Visual Science

Article Title: Compositional Analysis of Extracellular Aggregates in the Eyes of Patients With Exfoliation Syndrome and Exfoliation Glaucoma

doi: 10.1167/iovs.62.15.27

Figure Lengend Snippet: Immunofluorescence localization of LOXL1 and FBN1 on the surface of a lens capsule specimen from a patient with XFS (#K28). ( A – C ) Both LOXL1 ( red ) and FBN1 ( green ) label XFM in the central disc (CD) and granular zone (GZ). The clear zone (CZ), which lacks XFM, is unlabeled. The two immunofluorescence signals largely overlap, as shown in the merged image ( C ). However, high magnification images ( D – F ) of an individual XFM aggregate from the GZ shows that the two proteins are not precisely colocalized. Similarly, antibodies to the N -terminal ( G ; green ) or C -terminal ( H ; red ) regions of LOXL1 label XFM aggregates but the two signals only partially overlap ( I ).

Article Snippet: LOXL1 catalytic domain , Polyclonal , 1:100 , PB9719 , Boster, Pleasanton, CA.

Techniques: Immunofluorescence

Journal: Investigative Ophthalmology & Visual Science

Article Title: Compositional Analysis of Extracellular Aggregates in the Eyes of Patients With Exfoliation Syndrome and Exfoliation Glaucoma

doi: 10.1167/iovs.62.15.27

Figure Lengend Snippet: Relative abundance of LEFTY2, BIGLYCAN, and LOXL1 in AH samples from control patients (CAT and CAT/GL), a patient with XFS, and patients with XFG.

Article Snippet: LOXL1 catalytic domain , Polyclonal , 1:100 , PB9719 , Boster, Pleasanton, CA.

Techniques: Control

Journal: Cell Reports

Article Title: NR4A Nuclear Receptors Target Poly-ADP-Ribosylated DNA-PKcs Protein to Promote DNA Repair

doi: 10.1016/j.celrep.2019.01.083

Figure Lengend Snippet:

Article Snippet: Rabbit Anti-NR4A1 , BosterBio , Cat# PB9766.

Techniques: Recombinant, SYBR Green Assay, Fractionation, Luciferase, Stable Transfection, Expressing, Plasmid Preparation, Software

Journal: International Journal of Molecular Sciences

Article Title: Suitability of GRK Antibodies for Individual Detection and Quantification of GRK Isoforms in Western Blots

doi: 10.3390/ijms23031195

Figure Lengend Snippet: List of eight commercially available antibodies examined in this study, targeting the ubiquitously expressed human GRK isoforms. Overview of the tested antibodies (monoclonal (mc), polyclonal (pc)) and the tested GRK isoform is provided, including the supplier’s information, our review of each antibody, and the dilution used in Western blot to determine the antibody specificity. Additionally, the number of amino acids (aa) and the calculated approximate molecular weight (MW) for each human GRK isoform are listed. Accession numbers for protein isoforms can be found in the Materials and Methods section and additionally summarized in Supplementary Table S1 .

Article Snippet: The utilized GRK antibodies are listed in , the Boster Biological Technology GRK6 antibody PB9709 was obtained from Antibodies-Online GmbH, Aachen, Germany with ordering number ABIN3042435, actin antibody was purchased from Sigma-Aldrich (Taufkirchen, Germany, A5441, dilution 1:5000), vinculin antibody was obtained from Biozol (Eching, Germany, BZL03106, dilution 1:1000).

Techniques: Western Blot, Molecular Weight

Journal: International Journal of Molecular Sciences

Article Title: Suitability of GRK Antibodies for Individual Detection and Quantification of GRK Isoforms in Western Blots

doi: 10.3390/ijms23031195

Figure Lengend Snippet: GRK isoform specificity of several commercially available GRK antibodies. HEK293 cells were transiently transfected with pcDNA3 plasmids encoding either GRK2, GRK3-1, GRK3-2, GRK5, GRK6-1, GRK6-2, GRK6-3, or GRK6-4 as indicated. GRK isoform specificity of indicated GRK antibodies was investigated in Western blot analysis. Representative blots are shown. Two different antibodies raised against GRK2 ( a , b ), GRK3 ( c , d ), GRK5 ( e , f ), and GRK6 ( g , h ) were used for the analysis. Approximate molecular weight of each antigen is stated. Detailed information on each antibody examined is listed in . Actin served as loading control.

Article Snippet: The utilized GRK antibodies are listed in , the Boster Biological Technology GRK6 antibody PB9709 was obtained from Antibodies-Online GmbH, Aachen, Germany with ordering number ABIN3042435, actin antibody was purchased from Sigma-Aldrich (Taufkirchen, Germany, A5441, dilution 1:5000), vinculin antibody was obtained from Biozol (Eching, Germany, BZL03106, dilution 1:1000).

Techniques: Transfection, Western Blot, Molecular Weight

Journal: International Journal of Molecular Sciences

Article Title: Suitability of GRK Antibodies for Individual Detection and Quantification of GRK Isoforms in Western Blots

doi: 10.3390/ijms23031195

Figure Lengend Snippet: STARPA reveals differential GRK expression in commonly used cell lines. ( a ) STARPA was used to determine relative GRK expression in HEK293, HeLa, HepG2, Jurkat, K562, MCF-7, Molm-13, U2OS, and U-251 MG cells. Lysates of different cell lines together with the validated STARPA standards ( c,d) were loaded onto polyacrylamide gels. Western blot analysis using anti-HA antibody confirmed the STARPA standards. Immunoblotting using anti-GRK antibodies for each isoform allowed the determination of endogenously expressed GRK levels in the cell lines. Shown are representative images of three independent blots from identical lysates. ( b ) Close ups of the STARPA standards from the immunoblot presented in ( a ) displaying the cross-reactivity of the anti-GRK2 and anti-GRK6 antibody towards GRK3 and GRK5, respectively. Below, the quantification of three independent blots ± SD. The means of the obtained signals are indicated and were used to determine the respective cross-reactivity coefficient of the antibodies. ( c ) STARPA blots were quantified according to each GRK isoform-specific antibody, and the relative signal compared to the respective signal in the standards is shown as mean + SD of three independent blots from identical lysates. The calculated relative expression levels of GRK2 and GRK6 before (striped) and after (solid) cross-reactivity correction are presented.

Article Snippet: The utilized GRK antibodies are listed in , the Boster Biological Technology GRK6 antibody PB9709 was obtained from Antibodies-Online GmbH, Aachen, Germany with ordering number ABIN3042435, actin antibody was purchased from Sigma-Aldrich (Taufkirchen, Germany, A5441, dilution 1:5000), vinculin antibody was obtained from Biozol (Eching, Germany, BZL03106, dilution 1:1000).

Techniques: Expressing, Western Blot