PASE Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Thermo Fisher acid solution
    Acid Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acid solution/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    acid solution - by Bioz Stars, 2022-01
    93/100 stars
      Buy from Supplier

    93
    Abcam anti glucose 6 phosphatase catalytic subunit antibody
    Anti Glucose 6 Phosphatase Catalytic Subunit Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti glucose 6 phosphatase catalytic subunit antibody/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti glucose 6 phosphatase catalytic subunit antibody - by Bioz Stars, 2022-01
    93/100 stars
      Buy from Supplier

    94
    Abcam anti psap
    Immunofluorescence light micrographs stained with commercial antibodies against <t>GPR37</t> (purple)/GPR37L1 (green) at 4 and 8 weeks after birth. The results were similar to those obtained with our autonomous antibodies; fine granular <t>PSAP</t> in satellite cells always colocalized with both GPR-37 and GPR-37L1 in the adult DRG (e–h); in young DRG neurons, PSAP did not always colocalize with receptors (a–d). In (b), only half of the satellite cells were outlined with fine granular PSAP (white arrowheads).
    Anti Psap, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti psap/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti psap - by Bioz Stars, 2022-01
    94/100 stars
      Buy from Supplier

    Image Search Results


    Immunofluorescence light micrographs stained with commercial antibodies against GPR37 (purple)/GPR37L1 (green) at 4 and 8 weeks after birth. The results were similar to those obtained with our autonomous antibodies; fine granular PSAP in satellite cells always colocalized with both GPR-37 and GPR-37L1 in the adult DRG (e–h); in young DRG neurons, PSAP did not always colocalize with receptors (a–d). In (b), only half of the satellite cells were outlined with fine granular PSAP (white arrowheads).

    Journal: PLoS ONE

    Article Title: The expression of prosaposin and its receptors, GRP37 and GPR37L1, are increased in the developing dorsal root ganglion

    doi: 10.1371/journal.pone.0255958

    Figure Lengend Snippet: Immunofluorescence light micrographs stained with commercial antibodies against GPR37 (purple)/GPR37L1 (green) at 4 and 8 weeks after birth. The results were similar to those obtained with our autonomous antibodies; fine granular PSAP in satellite cells always colocalized with both GPR-37 and GPR-37L1 in the adult DRG (e–h); in young DRG neurons, PSAP did not always colocalize with receptors (a–d). In (b), only half of the satellite cells were outlined with fine granular PSAP (white arrowheads).

    Article Snippet: Following antigen retrieval, sections were incubated in blocking solution containing 5% NSS, 5% BSA, and 0.25% carrageenan in PBS for 2 h. The sections were processed for immunohistochemistry with anti-PSAP (IM-1), anti-GPR37 or anti-GPR37L, and anti-sortilin (Abcam, Tokyo, Japan) antibodies at a concentration of 1 μg/ml overnight at 4°C.

    Techniques: Immunofluorescence, Staining

    Immunofluorescence light micrographs of the rat DRG from day 1 to 2.5 years after birth stained with anti-PSAP (IM-1, red), anti-GPR37 (blue), and anti-GPR37L1 (green). Before week 1 (a–c), staining was weak, except for some small and damaged neurons (arrowheads in c and white arrows in b, respectively). From 2–12 weeks (d–i), some satellite cells showed strong staining of GPR37L1 (arrowheads in d–k). The area in the rectangle in Fig 5e is shown in higher magnification view (f1–f4). After 16 weeks, these cells were rare. Asterisks indicate the nuclei of satellite cells. Arrows in Fig 5k and 5l indicate lipofuscin granules with strong fluorescence. The numbers on the bars indicate the scale in μm.

    Journal: PLoS ONE

    Article Title: The expression of prosaposin and its receptors, GRP37 and GPR37L1, are increased in the developing dorsal root ganglion

    doi: 10.1371/journal.pone.0255958

    Figure Lengend Snippet: Immunofluorescence light micrographs of the rat DRG from day 1 to 2.5 years after birth stained with anti-PSAP (IM-1, red), anti-GPR37 (blue), and anti-GPR37L1 (green). Before week 1 (a–c), staining was weak, except for some small and damaged neurons (arrowheads in c and white arrows in b, respectively). From 2–12 weeks (d–i), some satellite cells showed strong staining of GPR37L1 (arrowheads in d–k). The area in the rectangle in Fig 5e is shown in higher magnification view (f1–f4). After 16 weeks, these cells were rare. Asterisks indicate the nuclei of satellite cells. Arrows in Fig 5k and 5l indicate lipofuscin granules with strong fluorescence. The numbers on the bars indicate the scale in μm.

    Article Snippet: Following antigen retrieval, sections were incubated in blocking solution containing 5% NSS, 5% BSA, and 0.25% carrageenan in PBS for 2 h. The sections were processed for immunohistochemistry with anti-PSAP (IM-1), anti-GPR37 or anti-GPR37L, and anti-sortilin (Abcam, Tokyo, Japan) antibodies at a concentration of 1 μg/ml overnight at 4°C.

    Techniques: Immunofluorescence, Staining, Fluorescence

    (a–f) Immunofluorescence light micrographs of the rat DRG from 3 days to 8 weeks after birth, stained with anti-PSAP (red), anti-sortilin (Solt, green), and DAPI. Weak sortilin-IR was first observed at postnatal day 7 in satellite cells (arrows in b). After postnatal week 2, the number of sortilin-positive cells increased, peaking at postnatal week 4 (arrows in d) and decreasing after postnatal week 8 (f). (g–o) Higher magnification immunofluorescence light micrographs of the rat DRG at 8 weeks after birth stained with antibodies against PSAP (red), sortilin (green), and DAPI (blue). (g, j) Both the outer and inner cell surface of satellite cells were clearly outlined by sortilin, while only the inner surface of satellite cells was outlined with fine granular PSAP (white arrowheads in i, l, and o), and not the outer cell surface (black arrowheads in i, l). (m) Rare case of a thin satellite cell (S) between two neurons (N), indicating clear colocalization of sortilin (n) and PSAP (o). (p–x) The relationships of PSAP and its receptors (GPR37, GPR37L1) with sortilin was studied by double immunofluorescence of the DRG at postnatal week 4. Sortilin-IR was localized in satellite cells, especially near the boundary to nerve cells (q, t, and w), while PSAP-IR (r) and GPR37-IR (u) were dispersed in the cytoplasm of satellite cells. Only GPR37L1 clearly colocalized with sortilin (w, x). The numbers on the bars indicate the scale in μm.

    Journal: PLoS ONE

    Article Title: The expression of prosaposin and its receptors, GRP37 and GPR37L1, are increased in the developing dorsal root ganglion

    doi: 10.1371/journal.pone.0255958

    Figure Lengend Snippet: (a–f) Immunofluorescence light micrographs of the rat DRG from 3 days to 8 weeks after birth, stained with anti-PSAP (red), anti-sortilin (Solt, green), and DAPI. Weak sortilin-IR was first observed at postnatal day 7 in satellite cells (arrows in b). After postnatal week 2, the number of sortilin-positive cells increased, peaking at postnatal week 4 (arrows in d) and decreasing after postnatal week 8 (f). (g–o) Higher magnification immunofluorescence light micrographs of the rat DRG at 8 weeks after birth stained with antibodies against PSAP (red), sortilin (green), and DAPI (blue). (g, j) Both the outer and inner cell surface of satellite cells were clearly outlined by sortilin, while only the inner surface of satellite cells was outlined with fine granular PSAP (white arrowheads in i, l, and o), and not the outer cell surface (black arrowheads in i, l). (m) Rare case of a thin satellite cell (S) between two neurons (N), indicating clear colocalization of sortilin (n) and PSAP (o). (p–x) The relationships of PSAP and its receptors (GPR37, GPR37L1) with sortilin was studied by double immunofluorescence of the DRG at postnatal week 4. Sortilin-IR was localized in satellite cells, especially near the boundary to nerve cells (q, t, and w), while PSAP-IR (r) and GPR37-IR (u) were dispersed in the cytoplasm of satellite cells. Only GPR37L1 clearly colocalized with sortilin (w, x). The numbers on the bars indicate the scale in μm.

    Article Snippet: Following antigen retrieval, sections were incubated in blocking solution containing 5% NSS, 5% BSA, and 0.25% carrageenan in PBS for 2 h. The sections were processed for immunohistochemistry with anti-PSAP (IM-1), anti-GPR37 or anti-GPR37L, and anti-sortilin (Abcam, Tokyo, Japan) antibodies at a concentration of 1 μg/ml overnight at 4°C.

    Techniques: Immunofluorescence, Staining

    DAB immunohistochemical analyses (a–f) and immunofluorescence (g–i) with antibodies against PSAP (IM-1) (a, b, g), GPR37 (c, d, h), and GPR37L1(e, f, i) in the DRG of rats at 8 weeks after birth. (b, d, and f) Higher magnification images of a, c, and e, respectively. Intense granular PSAP reactions were observed in small neurons, and large dot-like structures were observed in large neurons (b). Similar reactions were shown by immunofluorescence (g). GPR37 exhibited dot-like reactions that were not as strong as those observed with PSAP. GPR37L1 reactivity was similar to that of GPR37 in neurons, and was strong in satellite cells (arrows in e, f, and i), especially those around larger neurons. The numbers on the bars indicate the scale in μm.

    Journal: PLoS ONE

    Article Title: The expression of prosaposin and its receptors, GRP37 and GPR37L1, are increased in the developing dorsal root ganglion

    doi: 10.1371/journal.pone.0255958

    Figure Lengend Snippet: DAB immunohistochemical analyses (a–f) and immunofluorescence (g–i) with antibodies against PSAP (IM-1) (a, b, g), GPR37 (c, d, h), and GPR37L1(e, f, i) in the DRG of rats at 8 weeks after birth. (b, d, and f) Higher magnification images of a, c, and e, respectively. Intense granular PSAP reactions were observed in small neurons, and large dot-like structures were observed in large neurons (b). Similar reactions were shown by immunofluorescence (g). GPR37 exhibited dot-like reactions that were not as strong as those observed with PSAP. GPR37L1 reactivity was similar to that of GPR37 in neurons, and was strong in satellite cells (arrows in e, f, and i), especially those around larger neurons. The numbers on the bars indicate the scale in μm.

    Article Snippet: Following antigen retrieval, sections were incubated in blocking solution containing 5% NSS, 5% BSA, and 0.25% carrageenan in PBS for 2 h. The sections were processed for immunohistochemistry with anti-PSAP (IM-1), anti-GPR37 or anti-GPR37L, and anti-sortilin (Abcam, Tokyo, Japan) antibodies at a concentration of 1 μg/ml overnight at 4°C.

    Techniques: Immunohistochemistry, Immunofluorescence

    Schematic drawing of the interactions between neurons and satellite cells in the developing DRG. DRG neurons produced and contained PSAP in lysosomes with or without GPR37L1. At approximately 8 weeks, the satellite cells around larger neurons showed dispersed PSAP colocalized with both receptors near the neuron boundary. Sortilin, not shown in this figure, was observed at an earlier stage (2–4 weeks), and always colocalized with GPR37L1. In conclusion, DRG neurons produce PSAP, which may be delivered to satellite cells in a paracrine manner. It is also plausible that satellite cells take up PSAP from their surrounding extracellular space and transport PSAP to neurons. GPR37L1 and sortilin may transport PSAP to stimulate the proliferation or maturation of satellite cells.

    Journal: PLoS ONE

    Article Title: The expression of prosaposin and its receptors, GRP37 and GPR37L1, are increased in the developing dorsal root ganglion

    doi: 10.1371/journal.pone.0255958

    Figure Lengend Snippet: Schematic drawing of the interactions between neurons and satellite cells in the developing DRG. DRG neurons produced and contained PSAP in lysosomes with or without GPR37L1. At approximately 8 weeks, the satellite cells around larger neurons showed dispersed PSAP colocalized with both receptors near the neuron boundary. Sortilin, not shown in this figure, was observed at an earlier stage (2–4 weeks), and always colocalized with GPR37L1. In conclusion, DRG neurons produce PSAP, which may be delivered to satellite cells in a paracrine manner. It is also plausible that satellite cells take up PSAP from their surrounding extracellular space and transport PSAP to neurons. GPR37L1 and sortilin may transport PSAP to stimulate the proliferation or maturation of satellite cells.

    Article Snippet: Following antigen retrieval, sections were incubated in blocking solution containing 5% NSS, 5% BSA, and 0.25% carrageenan in PBS for 2 h. The sections were processed for immunohistochemistry with anti-PSAP (IM-1), anti-GPR37 or anti-GPR37L, and anti-sortilin (Abcam, Tokyo, Japan) antibodies at a concentration of 1 μg/ml overnight at 4°C.

    Techniques: Produced

    Immunofluorescence light micrographs of the rat DRG at 8 weeks, stained with antibodies against PSAP (red), GPR37L1 (green), and GPR37 (blue) (a–d). The neighbouring section was stained with antibodies against PSAP (red), GPR37 (green), and GPR37L1 (blue) (e–h). The intensity of GPR37L1-IR in satellite cells was stronger than that of GPR37-IR (c, h). The numbers on the bars indicate the scale in μm.

    Journal: PLoS ONE

    Article Title: The expression of prosaposin and its receptors, GRP37 and GPR37L1, are increased in the developing dorsal root ganglion

    doi: 10.1371/journal.pone.0255958

    Figure Lengend Snippet: Immunofluorescence light micrographs of the rat DRG at 8 weeks, stained with antibodies against PSAP (red), GPR37L1 (green), and GPR37 (blue) (a–d). The neighbouring section was stained with antibodies against PSAP (red), GPR37 (green), and GPR37L1 (blue) (e–h). The intensity of GPR37L1-IR in satellite cells was stronger than that of GPR37-IR (c, h). The numbers on the bars indicate the scale in μm.

    Article Snippet: Following antigen retrieval, sections were incubated in blocking solution containing 5% NSS, 5% BSA, and 0.25% carrageenan in PBS for 2 h. The sections were processed for immunohistochemistry with anti-PSAP (IM-1), anti-GPR37 or anti-GPR37L, and anti-sortilin (Abcam, Tokyo, Japan) antibodies at a concentration of 1 μg/ml overnight at 4°C.

    Techniques: Immunofluorescence, Staining