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    • snap tag protein  (New England Biolabs)
    92
    New England Biolabs snap tag protein
    Snap Tag Protein, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap tag protein/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap tag protein - by Bioz Stars, 2023-03
    92/100 stars
    		  Buy from Supplier
    snap f tag labelling  (New England Biolabs)
    86
    New England Biolabs snap f tag labelling
    ( a ) Combination of tris NTA labelling with <t>SNAP</t> <t>f</t> -tag labelling in living cells. HeLa Kyoto cells co-expressing His10 LaminA <t>and</t> <t>H2B-SNAP</t> f were squeezed in the presence of 100 nM tris NTA Alexa647 (magenta) and subsequently incubated with cell-permeable TMR-Star (red) for SNAP f -tag labelling. Confocal images were taken 1 h after squeezing and demonstrate simultaneous specific labelling of the His-tagged LaminA and the SNAP f -tagged H2B. ( b ) tris NTA Alexa64 7 concentration scan for tunable labelling of TAP1 mVenus-His10 in HeLa Kyoto cells. High labelling density was obtained even at 1 nM tris NTA Alexa647 during squeezing. ( c ) Light-activated labelling of His10-mEGFP Lamin A with PA- tris NTA ATTO565 in living HeLa Kyoto cells. Before photoactivation, no decoration of Lamin A was observed 24 h after squeezing (top), whereas on illumination fluorescence increase and specific labelling were monitored (bottom). ( d ) Reconstructed d STORM image of His10-mEGFP Lamin A labelled with tris NTA ATTO655 (100 nM) in a living HeLa Kyoto cell. Increased spatial resolution (≤40 nm) was obtained in live-cell super-resolution imaging of tris NTA ATTO655 by d STORM (left and magnification right) compared with the wide-field image (left corner, bottom). Images were taken by CLSM ( a – c ) or d STORM ( d ) 1 h ( a , b , d ) or 24 h ( c ) after squeezing. Dashed lines indicate the cell border. Scale bars, 5 μm ( a , b ), 10 μm ( c ) and 2 μm ( d ).
    Snap F Tag Labelling, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap f tag labelling/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap f tag labelling - by Bioz Stars, 2023-03
    86/100 stars
    		  Buy from Supplier
    snap tag  (New England Biolabs)
    86
    New England Biolabs snap tag
    ( a ) Combination of tris NTA labelling with <t>SNAP</t> <t>f</t> -tag labelling in living cells. HeLa Kyoto cells co-expressing His10 LaminA <t>and</t> <t>H2B-SNAP</t> f were squeezed in the presence of 100 nM tris NTA Alexa647 (magenta) and subsequently incubated with cell-permeable TMR-Star (red) for SNAP f -tag labelling. Confocal images were taken 1 h after squeezing and demonstrate simultaneous specific labelling of the His-tagged LaminA and the SNAP f -tagged H2B. ( b ) tris NTA Alexa64 7 concentration scan for tunable labelling of TAP1 mVenus-His10 in HeLa Kyoto cells. High labelling density was obtained even at 1 nM tris NTA Alexa647 during squeezing. ( c ) Light-activated labelling of His10-mEGFP Lamin A with PA- tris NTA ATTO565 in living HeLa Kyoto cells. Before photoactivation, no decoration of Lamin A was observed 24 h after squeezing (top), whereas on illumination fluorescence increase and specific labelling were monitored (bottom). ( d ) Reconstructed d STORM image of His10-mEGFP Lamin A labelled with tris NTA ATTO655 (100 nM) in a living HeLa Kyoto cell. Increased spatial resolution (≤40 nm) was obtained in live-cell super-resolution imaging of tris NTA ATTO655 by d STORM (left and magnification right) compared with the wide-field image (left corner, bottom). Images were taken by CLSM ( a – c ) or d STORM ( d ) 1 h ( a , b , d ) or 24 h ( c ) after squeezing. Dashed lines indicate the cell border. Scale bars, 5 μm ( a , b ), 10 μm ( c ) and 2 μm ( d ).
    Snap Tag, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap tag/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap tag - by Bioz Stars, 2023-03
    86/100 stars
    		  Buy from Supplier
    co purified go  (New England Biolabs)
    86
    New England Biolabs co purified go
    ( a ) Combination of tris NTA labelling with <t>SNAP</t> <t>f</t> -tag labelling in living cells. HeLa Kyoto cells co-expressing His10 LaminA <t>and</t> <t>H2B-SNAP</t> f were squeezed in the presence of 100 nM tris NTA Alexa647 (magenta) and subsequently incubated with cell-permeable TMR-Star (red) for SNAP f -tag labelling. Confocal images were taken 1 h after squeezing and demonstrate simultaneous specific labelling of the His-tagged LaminA and the SNAP f -tagged H2B. ( b ) tris NTA Alexa64 7 concentration scan for tunable labelling of TAP1 mVenus-His10 in HeLa Kyoto cells. High labelling density was obtained even at 1 nM tris NTA Alexa647 during squeezing. ( c ) Light-activated labelling of His10-mEGFP Lamin A with PA- tris NTA ATTO565 in living HeLa Kyoto cells. Before photoactivation, no decoration of Lamin A was observed 24 h after squeezing (top), whereas on illumination fluorescence increase and specific labelling were monitored (bottom). ( d ) Reconstructed d STORM image of His10-mEGFP Lamin A labelled with tris NTA ATTO655 (100 nM) in a living HeLa Kyoto cell. Increased spatial resolution (≤40 nm) was obtained in live-cell super-resolution imaging of tris NTA ATTO655 by d STORM (left and magnification right) compared with the wide-field image (left corner, bottom). Images were taken by CLSM ( a – c ) or d STORM ( d ) 1 h ( a , b , d ) or 24 h ( c ) after squeezing. Dashed lines indicate the cell border. Scale bars, 5 μm ( a , b ), 10 μm ( c ) and 2 μm ( d ).
    Co Purified Go, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/co purified go/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    co purified go - by Bioz Stars, 2023-03
    86/100 stars
    		  Buy from Supplier
    puri ed  (New England Biolabs)
    86
    New England Biolabs puri ed
    ( a ) Combination of tris NTA labelling with <t>SNAP</t> <t>f</t> -tag labelling in living cells. HeLa Kyoto cells co-expressing His10 LaminA <t>and</t> <t>H2B-SNAP</t> f were squeezed in the presence of 100 nM tris NTA Alexa647 (magenta) and subsequently incubated with cell-permeable TMR-Star (red) for SNAP f -tag labelling. Confocal images were taken 1 h after squeezing and demonstrate simultaneous specific labelling of the His-tagged LaminA and the SNAP f -tagged H2B. ( b ) tris NTA Alexa64 7 concentration scan for tunable labelling of TAP1 mVenus-His10 in HeLa Kyoto cells. High labelling density was obtained even at 1 nM tris NTA Alexa647 during squeezing. ( c ) Light-activated labelling of His10-mEGFP Lamin A with PA- tris NTA ATTO565 in living HeLa Kyoto cells. Before photoactivation, no decoration of Lamin A was observed 24 h after squeezing (top), whereas on illumination fluorescence increase and specific labelling were monitored (bottom). ( d ) Reconstructed d STORM image of His10-mEGFP Lamin A labelled with tris NTA ATTO655 (100 nM) in a living HeLa Kyoto cell. Increased spatial resolution (≤40 nm) was obtained in live-cell super-resolution imaging of tris NTA ATTO655 by d STORM (left and magnification right) compared with the wide-field image (left corner, bottom). Images were taken by CLSM ( a – c ) or d STORM ( d ) 1 h ( a , b , d ) or 24 h ( c ) after squeezing. Dashed lines indicate the cell border. Scale bars, 5 μm ( a , b ), 10 μm ( c ) and 2 μm ( d ).
    Puri Ed, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/puri ed/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    puri ed - by Bioz Stars, 2023-03
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a ) Combination of tris NTA labelling with SNAP f -tag labelling in living cells. HeLa Kyoto cells co-expressing His10 LaminA and H2B-SNAP f were squeezed in the presence of 100 nM tris NTA Alexa647 (magenta) and subsequently incubated with cell-permeable TMR-Star (red) for SNAP f -tag labelling. Confocal images were taken 1 h after squeezing and demonstrate simultaneous specific labelling of the His-tagged LaminA and the SNAP f -tagged H2B. ( b ) tris NTA Alexa64 7 concentration scan for tunable labelling of TAP1 mVenus-His10 in HeLa Kyoto cells. High labelling density was obtained even at 1 nM tris NTA Alexa647 during squeezing. ( c ) Light-activated labelling of His10-mEGFP Lamin A with PA- tris NTA ATTO565 in living HeLa Kyoto cells. Before photoactivation, no decoration of Lamin A was observed 24 h after squeezing (top), whereas on illumination fluorescence increase and specific labelling were monitored (bottom). ( d ) Reconstructed d STORM image of His10-mEGFP Lamin A labelled with tris NTA ATTO655 (100 nM) in a living HeLa Kyoto cell. Increased spatial resolution (≤40 nm) was obtained in live-cell super-resolution imaging of tris NTA ATTO655 by d STORM (left and magnification right) compared with the wide-field image (left corner, bottom). Images were taken by CLSM ( a – c ) or d STORM ( d ) 1 h ( a , b , d ) or 24 h ( c ) after squeezing. Dashed lines indicate the cell border. Scale bars, 5 μm ( a , b ), 10 μm ( c ) and 2 μm ( d ).

    Journal: Nature Communications

    Article Title: Live-cell protein labelling with nanometre precision by cell squeezing

    doi: 10.1038/ncomms10372

    Figure Lengend Snippet: ( a ) Combination of tris NTA labelling with SNAP f -tag labelling in living cells. HeLa Kyoto cells co-expressing His10 LaminA and H2B-SNAP f were squeezed in the presence of 100 nM tris NTA Alexa647 (magenta) and subsequently incubated with cell-permeable TMR-Star (red) for SNAP f -tag labelling. Confocal images were taken 1 h after squeezing and demonstrate simultaneous specific labelling of the His-tagged LaminA and the SNAP f -tagged H2B. ( b ) tris NTA Alexa64 7 concentration scan for tunable labelling of TAP1 mVenus-His10 in HeLa Kyoto cells. High labelling density was obtained even at 1 nM tris NTA Alexa647 during squeezing. ( c ) Light-activated labelling of His10-mEGFP Lamin A with PA- tris NTA ATTO565 in living HeLa Kyoto cells. Before photoactivation, no decoration of Lamin A was observed 24 h after squeezing (top), whereas on illumination fluorescence increase and specific labelling were monitored (bottom). ( d ) Reconstructed d STORM image of His10-mEGFP Lamin A labelled with tris NTA ATTO655 (100 nM) in a living HeLa Kyoto cell. Increased spatial resolution (≤40 nm) was obtained in live-cell super-resolution imaging of tris NTA ATTO655 by d STORM (left and magnification right) compared with the wide-field image (left corner, bottom). Images were taken by CLSM ( a – c ) or d STORM ( d ) 1 h ( a , b , d ) or 24 h ( c ) after squeezing. Dashed lines indicate the cell border. Scale bars, 5 μm ( a , b ), 10 μm ( c ) and 2 μm ( d ).

    Article Snippet: The pSNAP f -H2B and pSNAP f -Cox8A plasmids (New England Biolabs) were used for SNAP f -tag labelling.

    Techniques: Expressing, Incubation, Concentration Assay, Fluorescence, Imaging