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Image Search Results
Journal: Advanced Science
Article Title: Trip13 Depletion in Liver Cancer Induces a Lipogenic Response Contributing to Plin2‐Dependent Mitotic Cell Death
doi: 10.1002/advs.202104291
Figure Lengend Snippet: Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes (p350) ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.
Article Snippet: Plin1 (ab61682), Plin2 (ab108323 and ab52356), Plin3 (ab47638), Plin4 (ab234752), Plin5 (ab222811), sterol regulatory‐element binding protein (SREBP) (ab28481), Mad2 (ab97777), GFP (ab290), pericentrin (ab4448), CD36 (ab133625), tubulin (ab7291), and valosin‐containing protein (VCP) (ab11433) were ordered from abcam,
Techniques: Control, Transfection, Western Blot, Targeted Proteomics, Comparison