P-350 Search Results


93
Alomone Labs pnu120596
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Proteintech dna
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Becton Dickinson mouse anti-dna-pkcs (p350 6
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Verlag GmbH quoted from anette herlitzius, frauenbefreiung und rassenideologie, wiesbaden, deutscher universitäts verlag, 1995, p. 350
Quoted From Anette Herlitzius, Frauenbefreiung Und Rassenideologie, Wiesbaden, Deutscher Universitäts Verlag, 1995, P. 350, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/quoted from anette herlitzius, frauenbefreiung und rassenideologie, wiesbaden, deutscher universitäts verlag, 1995, p. 350/product/Verlag GmbH
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Lotek Wireless Inc pinpoint p-350 (17 g)
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Biozol Diagnostica Vertrieb GmbH p350 ata-amab91164-100
Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes <t>(p350)</t> ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.
P350 Ata Amab91164 100, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CEM Corporation cem-p 350
Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes <t>(p350)</t> ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.
Cem P 350, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Hypercube Inc pentium p350 pc
Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes <t>(p350)</t> ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.
Pentium P350 Pc, supplied by Hypercube Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Conoptics Inc pockels cell electro-optic modulator kd*p 350-50
Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes <t>(p350)</t> ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.
Pockels Cell Electro Optic Modulator Kd*P 350 50, supplied by Conoptics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Schering-Plough corporation p350 dilatrend prescribing information
Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes <t>(p350)</t> ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.
P350 Dilatrend Prescribing Information, supplied by Schering-Plough corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes (p350) ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.

Journal: Advanced Science

Article Title: Trip13 Depletion in Liver Cancer Induces a Lipogenic Response Contributing to Plin2‐Dependent Mitotic Cell Death

doi: 10.1002/advs.202104291

Figure Lengend Snippet: Prevention of Trip13‐KD‐induced accumulation of lipid droplets rescues mitotic abnormalities and cell death. A) Representative images of HLF control cells and cells upon KD of Trip13 and/or insulin receptor showing lipid droplets and mitotic spindles. Red (Aurora kinase A), green (bodipy), blue (DAPI) ( n = 3). B) Quantification of the proportion of cells displaying lipid droplets transfected as in (A). C) Quantification of the number of centrosomes in mitotic HLF cells. D) Representative images of control and Trip13 KD HLF cells showing lipid droplets (Bodipy) and centrosomes (p350) ( n = 3). E) Number of MTOCs formed during mitosis in Trip13 KD cells. The green bar represents the time of metaphase initiation in control cells, the red bar represents the time of anaphase in control cells ( n = 5). F) Western blots showing FASN, ACC, and pACLY in Trip13 KD HLF cells ( n = 4). G) Levels of cleaved caspase 3, pH2Ax, and pH3 in HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). H) Representative images of HLF cells with KD of Trip13 alone or in combination with ACLY KD showing spindle abnormalities and lipid droplet accumulation ( n = 3). I) Growth curve of HLF control cells or upon KD of Trip13 and/or ACLY ( n = 3). J) Mitotic index of HLF cells upon Trip13 KD alone or in combination with KD of ACLY, ACC, or AGPAT2 ( n = 3). K) Percentage of HLF cells displaying lipid droplets transfected as in (J) ( n = 3). L) Lipidomic analysis showing log2 fold changes of TG concentrations between Trip13 KD (siTrip13) and control (siControl) HLF cells at 30 h post‐transfection ( n = 5). The x ‐axis represents triglycerides (TGs) with number of carbon atoms and number of double bonds. The y ‐axis indicates one fatty acid (out of three per TG) that can be specified using the multiple reaction monitoring (MRM)‐based Lipidyzer assay. (B, C, J, K) Data are shown as the mean ± SEM and analyzed by 1‐way ANOVA with Tukey's Multiple Comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001. (L) Data were analyzed using unpaired Wilcoxon rank‐sum tests corrected for multiple comparisons using the Benjamini–Hochberg method. q values <0.05 (*) were considered as statistically significant. n numbers refer to biological replicates. See also Figure in the Supporting Information.

Article Snippet: Plin1 (ab61682), Plin2 (ab108323 and ab52356), Plin3 (ab47638), Plin4 (ab234752), Plin5 (ab222811), sterol regulatory‐element binding protein (SREBP) (ab28481), Mad2 (ab97777), GFP (ab290), pericentrin (ab4448), CD36 (ab133625), tubulin (ab7291), and valosin‐containing protein (VCP) (ab11433) were ordered from abcam, P350 (ATA‐AMAB91164‐100) from Biozol, Trip13 (19602‐1‐AP) from Proteintech, and INSR (sc‐711), FASN (sc‐20140) from SantaCruz Biotech.

Techniques: Control, Transfection, Western Blot, Targeted Proteomics, Comparison