P-155 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    BioLegend cd163
    Macrophages are increased in pancreatic cancer and express Csf1R. a ) Expression of CSF1 , CSF2 , and CSF3 in resected human PDA (n=11) and normal adjacent pancreas (Nadj, n=3). Data are mean ± SEM. PanCancer Immune Profiling (Nanostring) was performed once. b ) Correlations between expression CSF1, CSF1R , and <t>CD163</t> in human PDA was obtained from TCGA (n=165 samples). c ) Flow cytometric staining of CD115 on CD11b + CD163 + and CD11b + CD163 − myeloid cells isolated from resected human PDA. Representative of n=4 independent patient samples. d ) Frequency of CD115 + cells among CD11b + CD163 + ). e ) CD11b + (myeloid) and CK + tumor cell staining in KPC pancreatic intraepithelial neoplasms (PanIN), primary tumors (PDA), and a diaphragm metastasis. Scale bar, 25 μm. f ) Fold-increase in gene expression in human PDA (hPDA) vs. mouse PDA (mPDA) determined by comparison to gene expression in normal adjacent pancreas (hPDA) or normal pancreas from wild-type mice (mPDA). g ) Frequency of granulocytes (red box, CD11b + Gr1 high Ly6C int ), Ly6C high TAMs (green box, CD11b + Gr1 int Ly6C high ), and Ly6C low TAMs (blue box, CD11b + Gr1 − Ly6C − ) in spleen and tumor isolated from a representative KPC mouse. Plots are gated on CD45 + CD11b + cells. Data are representative of > 10 KPC tumors analyzed in a similar manner. h ) Expression of the indicated markers on gated CD19 + B cells, NK cells, Ly6C low TAMs, granuolyctes, and Ly6C high TAMs. *, p
    Cd163, supplied by BioLegend, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd163/product/BioLegend
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd163 - by Bioz Stars, 2022-07
    93/100 stars
      Buy from Supplier

    80
    Abcam multimerin 1
    (a) The Mass spectrometry (MS)/MS spectra of selected tryptic peptides FVLVQENRPTLTDIVELR from isoform-1 of <t>multimerin-1;</t> (b) The MS/MS spectra of selected tryptic peptides IQNLGLFPIHGMMMK from integrin alpha-11.
    Multimerin 1, supplied by Abcam, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multimerin 1/product/Abcam
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    multimerin 1 - by Bioz Stars, 2022-07
    80/100 stars
      Buy from Supplier

    93
    BioLegend anti cd163
    (a) The Mass spectrometry (MS)/MS spectra of selected tryptic peptides FVLVQENRPTLTDIVELR from isoform-1 of <t>multimerin-1;</t> (b) The MS/MS spectra of selected tryptic peptides IQNLGLFPIHGMMMK from integrin alpha-11.
    Anti Cd163, supplied by BioLegend, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd163/product/BioLegend
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd163 - by Bioz Stars, 2022-07
    93/100 stars
      Buy from Supplier

    88
    Santa Cruz Biotechnology multimerin 1
    Efb interacts with platelet P-selectin. A , human platelets were ultrasonicated in the presence or absence of 3 mg/ml added fibrinogen. Sigma His-Select HF nickel affinity gel was used to capture His-tagged Efb- or Efb-N-interacting proteins from platelet lysates. Affinity precipitates were separated by SDS-PAGE and immunoblotted for integrin αIIb, P-selectin, <t>multimerin-1,</t> fibronectin, thrombospondin-1 (indicated by Thrombosp.-1 ), and His tag (to confirm Efb/Efb-N expression and immobilization). Immunoblots shown here are representative of those from three independent experiments. -ve , negative control; PD , pulldown. B , SPR data confirm the direct interaction of Efb and P-selectin. Recombinant P-selectin-Fc immobilized on COOHV-coated SensiQ TM sensor chips was treated with increasing concentrations of Efb (100 n m ( brown ), 300 n m ( purple ), and 2.8 μ m ( green )). All experiments were performed using the SensiQ TM Pioneer FE SPR platform (SensiQ Technologies). Experimental data were fit using the Qdat data analysis software. Curves were fitted using a least squares fitting algorithm. RU , response units.
    Multimerin 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multimerin 1/product/Santa Cruz Biotechnology
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    multimerin 1 - by Bioz Stars, 2022-07
    88/100 stars
      Buy from Supplier

    Image Search Results


    Macrophages are increased in pancreatic cancer and express Csf1R. a ) Expression of CSF1 , CSF2 , and CSF3 in resected human PDA (n=11) and normal adjacent pancreas (Nadj, n=3). Data are mean ± SEM. PanCancer Immune Profiling (Nanostring) was performed once. b ) Correlations between expression CSF1, CSF1R , and CD163 in human PDA was obtained from TCGA (n=165 samples). c ) Flow cytometric staining of CD115 on CD11b + CD163 + and CD11b + CD163 − myeloid cells isolated from resected human PDA. Representative of n=4 independent patient samples. d ) Frequency of CD115 + cells among CD11b + CD163 + ). e ) CD11b + (myeloid) and CK + tumor cell staining in KPC pancreatic intraepithelial neoplasms (PanIN), primary tumors (PDA), and a diaphragm metastasis. Scale bar, 25 μm. f ) Fold-increase in gene expression in human PDA (hPDA) vs. mouse PDA (mPDA) determined by comparison to gene expression in normal adjacent pancreas (hPDA) or normal pancreas from wild-type mice (mPDA). g ) Frequency of granulocytes (red box, CD11b + Gr1 high Ly6C int ), Ly6C high TAMs (green box, CD11b + Gr1 int Ly6C high ), and Ly6C low TAMs (blue box, CD11b + Gr1 − Ly6C − ) in spleen and tumor isolated from a representative KPC mouse. Plots are gated on CD45 + CD11b + cells. Data are representative of > 10 KPC tumors analyzed in a similar manner. h ) Expression of the indicated markers on gated CD19 + B cells, NK cells, Ly6C low TAMs, granuolyctes, and Ly6C high TAMs. *, p

    Journal: Cancer immunology research

    Article Title: Differential effects of depleting versus programming tumor-associated macrophages on engineered T cells in pancreatic ductal adenocarcinoma

    doi: 10.1158/2326-6066.CIR-18-0448

    Figure Lengend Snippet: Macrophages are increased in pancreatic cancer and express Csf1R. a ) Expression of CSF1 , CSF2 , and CSF3 in resected human PDA (n=11) and normal adjacent pancreas (Nadj, n=3). Data are mean ± SEM. PanCancer Immune Profiling (Nanostring) was performed once. b ) Correlations between expression CSF1, CSF1R , and CD163 in human PDA was obtained from TCGA (n=165 samples). c ) Flow cytometric staining of CD115 on CD11b + CD163 + and CD11b + CD163 − myeloid cells isolated from resected human PDA. Representative of n=4 independent patient samples. d ) Frequency of CD115 + cells among CD11b + CD163 + ). e ) CD11b + (myeloid) and CK + tumor cell staining in KPC pancreatic intraepithelial neoplasms (PanIN), primary tumors (PDA), and a diaphragm metastasis. Scale bar, 25 μm. f ) Fold-increase in gene expression in human PDA (hPDA) vs. mouse PDA (mPDA) determined by comparison to gene expression in normal adjacent pancreas (hPDA) or normal pancreas from wild-type mice (mPDA). g ) Frequency of granulocytes (red box, CD11b + Gr1 high Ly6C int ), Ly6C high TAMs (green box, CD11b + Gr1 int Ly6C high ), and Ly6C low TAMs (blue box, CD11b + Gr1 − Ly6C − ) in spleen and tumor isolated from a representative KPC mouse. Plots are gated on CD45 + CD11b + cells. Data are representative of > 10 KPC tumors analyzed in a similar manner. h ) Expression of the indicated markers on gated CD19 + B cells, NK cells, Ly6C low TAMs, granuolyctes, and Ly6C high TAMs. *, p

    Article Snippet: Human antibodies used included CD11b (ICRF-44; eBioscience), CD115 (12-3A3-1B10; eBioscience), and CD163 (GH1/61; Biolegend).

    Techniques: Expressing, Staining, Isolation, Mouse Assay

    (a) The Mass spectrometry (MS)/MS spectra of selected tryptic peptides FVLVQENRPTLTDIVELR from isoform-1 of multimerin-1; (b) The MS/MS spectra of selected tryptic peptides IQNLGLFPIHGMMMK from integrin alpha-11.

    Journal: Chinese Medical Journal

    Article Title: Proteomic Analysis for Finding Serum Pathogenic Factors and Potential Biomarkers in Multiple Myeloma

    doi: 10.4103/0366-6999.155112

    Figure Lengend Snippet: (a) The Mass spectrometry (MS)/MS spectra of selected tryptic peptides FVLVQENRPTLTDIVELR from isoform-1 of multimerin-1; (b) The MS/MS spectra of selected tryptic peptides IQNLGLFPIHGMMMK from integrin alpha-11.

    Article Snippet: Isoform-1 of multimerin-1 appeared to be down-regulated in MM patients at (3019 ± 641) versus controls at (11,988 ± 846) P < 0.01).

    Techniques: Mass Spectrometry

    Efb interacts with platelet P-selectin. A , human platelets were ultrasonicated in the presence or absence of 3 mg/ml added fibrinogen. Sigma His-Select HF nickel affinity gel was used to capture His-tagged Efb- or Efb-N-interacting proteins from platelet lysates. Affinity precipitates were separated by SDS-PAGE and immunoblotted for integrin αIIb, P-selectin, multimerin-1, fibronectin, thrombospondin-1 (indicated by Thrombosp.-1 ), and His tag (to confirm Efb/Efb-N expression and immobilization). Immunoblots shown here are representative of those from three independent experiments. -ve , negative control; PD , pulldown. B , SPR data confirm the direct interaction of Efb and P-selectin. Recombinant P-selectin-Fc immobilized on COOHV-coated SensiQ TM sensor chips was treated with increasing concentrations of Efb (100 n m ( brown ), 300 n m ( purple ), and 2.8 μ m ( green )). All experiments were performed using the SensiQ TM Pioneer FE SPR platform (SensiQ Technologies). Experimental data were fit using the Qdat data analysis software. Curves were fitted using a least squares fitting algorithm. RU , response units.

    Journal: The Journal of Biological Chemistry

    Article Title: Extracellular Fibrinogen-binding Protein (Efb) from Staphylococcus aureus Inhibits the Formation of Platelet-Leukocyte Complexes *

    doi: 10.1074/jbc.M115.678359

    Figure Lengend Snippet: Efb interacts with platelet P-selectin. A , human platelets were ultrasonicated in the presence or absence of 3 mg/ml added fibrinogen. Sigma His-Select HF nickel affinity gel was used to capture His-tagged Efb- or Efb-N-interacting proteins from platelet lysates. Affinity precipitates were separated by SDS-PAGE and immunoblotted for integrin αIIb, P-selectin, multimerin-1, fibronectin, thrombospondin-1 (indicated by Thrombosp.-1 ), and His tag (to confirm Efb/Efb-N expression and immobilization). Immunoblots shown here are representative of those from three independent experiments. -ve , negative control; PD , pulldown. B , SPR data confirm the direct interaction of Efb and P-selectin. Recombinant P-selectin-Fc immobilized on COOHV-coated SensiQ TM sensor chips was treated with increasing concentrations of Efb (100 n m ( brown ), 300 n m ( purple ), and 2.8 μ m ( green )). All experiments were performed using the SensiQ TM Pioneer FE SPR platform (SensiQ Technologies). Experimental data were fit using the Qdat data analysis software. Curves were fitted using a least squares fitting algorithm. RU , response units.

    Article Snippet: In addition to known interactors of Efb (i.e. fibrinogen, complement C3, and plasminogen) ( , , ), this analysis revealed novel potential partners of Efb, including functionally relevant proteins such as P-selectin (a platelet plasma membrane protein) and multimerin-1, emilin-1, fibronectin, and thrombospondin-1 (soluble extracellular proteins able to interact with cell adhesion receptors on platelets).

    Techniques: SDS Page, Expressing, Western Blot, Negative Control, SPR Assay, Recombinant, Software