Journal: The European journal of neuroscience

Article Title: Inflammation alters AMPA-stimulated calcium responses in dorsal striatal D2 but not D1 spiny projection neurons

doi: 10.1111/ejn.13711

Figure Lengend Snippet: Corticostriatal coronal sections were treated with 125 ng/ml LPS or vehicle for two hours. D2 or D1 SPNs were pretreated via y-tube application with philanthotoxin-74 (300 µM), TTX, and CdCl2, before exposure to the AMPA cocktail + philanthotoxin, TTX, and CdCl2. A) Peak amplitude of fluorescence response in D2 SPNs (****P < 0.0001), N = 3 animals per group and 39 slices. B) Peak amplitude of fluorescence response in D1 SPNs (****P < 0.0001), N = 3 animals per group and 49 slices.

Article Snippet: Studies employed commercially available chemicals as follows: S-AMPA (Abcam, # {"type":"entrez-nucleotide","attrs":{"text":"AB120005","term_id":"38194191","term_text":"AB120005"}} AB120005 ), cyclothiazide (Abcam, # {"type":"entrez-nucleotide","attrs":{"text":"AB120061","term_id":"119116622","term_text":"AB120061"}} AB120061 ), (R-)CPP (Abcam, # {"type":"entrez-nucleotide","attrs":{"text":"AB120159","term_id":"34740300","term_text":"AB120159"}} AB120159 ), TTX (Abcam, # {"type":"entrez-nucleotide","attrs":{"text":"AB120054","term_id":"45330725","term_text":"AB120054"}} AB120054 ), philanthotoxin-74 (Alomone Labs, #P-120), and isradipine (Tocris, #2004).

Techniques: Fluorescence

Journal: Scientific Reports

Article Title: GRP78 translocation to the cell surface and O-GlcNAcylation of VE-Cadherin contribute to ER stress-mediated endothelial permeability

doi: 10.1038/s41598-019-47246-w

Figure Lengend Snippet: HRECs exposed to TNF-α, and high glucose for 24 h demonstrated alterations in VE-Cadherin and GRP78 association. Whole cell lysate were immunoprecipitated with antibody against VE‐Cadherin, and the immunocomplexes were analyzed by immunoblotting for GRP78 ( A ). Whole cell lysates were immunoprecipitated with complex partners (p-120 and β-catenin) and immunoblotted with GRP78 ( B ). β-tubulin served as an internal control. Data represent Mean ± SEM from 3 independent experiments performed in duplicates. ***p < 0.001; **p < 0.01.

Article Snippet: p-120 , Abcam , ab72039.

Techniques: Immunoprecipitation, Western Blot