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  • 99
    Millipore calcofluor white
    Pro-CEP2 accumulates within the protoplasm of the longer most cells from the upper end of the LR cap (PCD site I) at the level of the transition zone and is still present in root cap corpses. Roots of 7 days old WT seedlings expressing the pro-mCherry-CEP2 (P CEP2 ::pre-pro-3xHA-mCherry-AtCEP2-KDEL) are stained with <t>calcofluor-white</t> in order to visualize the cell wall and analyzed by CLSM (stack of 4 optical sections, Olympus Fluoview FV 1000). Left: Visualization of cell walls (blue): isodiametric, not elongated epidermis cells (arrow heads) from the meristematic and transition zone as well as already elongating epidermis cells (arrows) from the rapid elongation zone in addition to small, elongated and already collapsing cells at the upper end of the LRC (asterisk) are found. Middle: Merge of calcofluor (blue) and pro-mCherry-CEP2 (red): pro-CEP2 is found in the protoplasm of LR cap cells and is still present in root cap corpses sticking to epidermis cells of the rapid elongation zone. Pro-CEP is localized within the cell in the protoplasm surrounding the large vacuole and not in the cell wall. Right: magnification of the white boxed area presenting a LR cap cell with the blue coloured cell wall, the large black looking vacuole and in between the protoplasm with the red coloured pro-CEP2.
    Calcofluor White, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calcofluor white/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calcofluor white - by Bioz Stars, 2022-01
    99/100 stars
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    86
    Agilent technologies d2 o
    Pro-CEP2 accumulates within the protoplasm of the longer most cells from the upper end of the LR cap (PCD site I) at the level of the transition zone and is still present in root cap corpses. Roots of 7 days old WT seedlings expressing the pro-mCherry-CEP2 (P CEP2 ::pre-pro-3xHA-mCherry-AtCEP2-KDEL) are stained with <t>calcofluor-white</t> in order to visualize the cell wall and analyzed by CLSM (stack of 4 optical sections, Olympus Fluoview FV 1000). Left: Visualization of cell walls (blue): isodiametric, not elongated epidermis cells (arrow heads) from the meristematic and transition zone as well as already elongating epidermis cells (arrows) from the rapid elongation zone in addition to small, elongated and already collapsing cells at the upper end of the LRC (asterisk) are found. Middle: Merge of calcofluor (blue) and pro-mCherry-CEP2 (red): pro-CEP2 is found in the protoplasm of LR cap cells and is still present in root cap corpses sticking to epidermis cells of the rapid elongation zone. Pro-CEP is localized within the cell in the protoplasm surrounding the large vacuole and not in the cell wall. Right: magnification of the white boxed area presenting a LR cap cell with the blue coloured cell wall, the large black looking vacuole and in between the protoplasm with the red coloured pro-CEP2.
    D2 O, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d2 o/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    d2 o - by Bioz Stars, 2022-01
    86/100 stars
      Buy from Supplier

    94
    Bruker Corporation h nmr spectrum
    Pro-CEP2 accumulates within the protoplasm of the longer most cells from the upper end of the LR cap (PCD site I) at the level of the transition zone and is still present in root cap corpses. Roots of 7 days old WT seedlings expressing the pro-mCherry-CEP2 (P CEP2 ::pre-pro-3xHA-mCherry-AtCEP2-KDEL) are stained with <t>calcofluor-white</t> in order to visualize the cell wall and analyzed by CLSM (stack of 4 optical sections, Olympus Fluoview FV 1000). Left: Visualization of cell walls (blue): isodiametric, not elongated epidermis cells (arrow heads) from the meristematic and transition zone as well as already elongating epidermis cells (arrows) from the rapid elongation zone in addition to small, elongated and already collapsing cells at the upper end of the LRC (asterisk) are found. Middle: Merge of calcofluor (blue) and pro-mCherry-CEP2 (red): pro-CEP2 is found in the protoplasm of LR cap cells and is still present in root cap corpses sticking to epidermis cells of the rapid elongation zone. Pro-CEP is localized within the cell in the protoplasm surrounding the large vacuole and not in the cell wall. Right: magnification of the white boxed area presenting a LR cap cell with the blue coloured cell wall, the large black looking vacuole and in between the protoplasm with the red coloured pro-CEP2.
    H Nmr Spectrum, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h nmr spectrum/product/Bruker Corporation
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h nmr spectrum - by Bioz Stars, 2022-01
    94/100 stars
      Buy from Supplier

    86
    Eppendorf AG d2 o
    Pro-CEP2 accumulates within the protoplasm of the longer most cells from the upper end of the LR cap (PCD site I) at the level of the transition zone and is still present in root cap corpses. Roots of 7 days old WT seedlings expressing the pro-mCherry-CEP2 (P CEP2 ::pre-pro-3xHA-mCherry-AtCEP2-KDEL) are stained with <t>calcofluor-white</t> in order to visualize the cell wall and analyzed by CLSM (stack of 4 optical sections, Olympus Fluoview FV 1000). Left: Visualization of cell walls (blue): isodiametric, not elongated epidermis cells (arrow heads) from the meristematic and transition zone as well as already elongating epidermis cells (arrows) from the rapid elongation zone in addition to small, elongated and already collapsing cells at the upper end of the LRC (asterisk) are found. Middle: Merge of calcofluor (blue) and pro-mCherry-CEP2 (red): pro-CEP2 is found in the protoplasm of LR cap cells and is still present in root cap corpses sticking to epidermis cells of the rapid elongation zone. Pro-CEP is localized within the cell in the protoplasm surrounding the large vacuole and not in the cell wall. Right: magnification of the white boxed area presenting a LR cap cell with the blue coloured cell wall, the large black looking vacuole and in between the protoplasm with the red coloured pro-CEP2.
    D2 O, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d2 o/product/Eppendorf AG
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    d2 o - by Bioz Stars, 2022-01
    86/100 stars
      Buy from Supplier

    Image Search Results


    Pro-CEP2 accumulates within the protoplasm of the longer most cells from the upper end of the LR cap (PCD site I) at the level of the transition zone and is still present in root cap corpses. Roots of 7 days old WT seedlings expressing the pro-mCherry-CEP2 (P CEP2 ::pre-pro-3xHA-mCherry-AtCEP2-KDEL) are stained with calcofluor-white in order to visualize the cell wall and analyzed by CLSM (stack of 4 optical sections, Olympus Fluoview FV 1000). Left: Visualization of cell walls (blue): isodiametric, not elongated epidermis cells (arrow heads) from the meristematic and transition zone as well as already elongating epidermis cells (arrows) from the rapid elongation zone in addition to small, elongated and already collapsing cells at the upper end of the LRC (asterisk) are found. Middle: Merge of calcofluor (blue) and pro-mCherry-CEP2 (red): pro-CEP2 is found in the protoplasm of LR cap cells and is still present in root cap corpses sticking to epidermis cells of the rapid elongation zone. Pro-CEP is localized within the cell in the protoplasm surrounding the large vacuole and not in the cell wall. Right: magnification of the white boxed area presenting a LR cap cell with the blue coloured cell wall, the large black looking vacuole and in between the protoplasm with the red coloured pro-CEP2.

    Journal: PLoS ONE

    Article Title: The role of KDEL-tailed cysteine endopeptidases of Arabidopsis (AtCEP2 and AtCEP1) in root development

    doi: 10.1371/journal.pone.0209407

    Figure Lengend Snippet: Pro-CEP2 accumulates within the protoplasm of the longer most cells from the upper end of the LR cap (PCD site I) at the level of the transition zone and is still present in root cap corpses. Roots of 7 days old WT seedlings expressing the pro-mCherry-CEP2 (P CEP2 ::pre-pro-3xHA-mCherry-AtCEP2-KDEL) are stained with calcofluor-white in order to visualize the cell wall and analyzed by CLSM (stack of 4 optical sections, Olympus Fluoview FV 1000). Left: Visualization of cell walls (blue): isodiametric, not elongated epidermis cells (arrow heads) from the meristematic and transition zone as well as already elongating epidermis cells (arrows) from the rapid elongation zone in addition to small, elongated and already collapsing cells at the upper end of the LRC (asterisk) are found. Middle: Merge of calcofluor (blue) and pro-mCherry-CEP2 (red): pro-CEP2 is found in the protoplasm of LR cap cells and is still present in root cap corpses sticking to epidermis cells of the rapid elongation zone. Pro-CEP is localized within the cell in the protoplasm surrounding the large vacuole and not in the cell wall. Right: magnification of the white boxed area presenting a LR cap cell with the blue coloured cell wall, the large black looking vacuole and in between the protoplasm with the red coloured pro-CEP2.

    Article Snippet: Roots of 7–10 days old seedlings were mounted on a microscope slide, incubated with propidium iodide (20 μg/ml H2 O; Sigma-Aldrich Chemie, Germany) or calcofluor-white (200 μl 500 mM Na-phosphate, 400 μl H2 O, 400 μl calcofluor-white stain pH 7.5; Sigma-Aldrich Chemie, Germany) and examined by CLSM.

    Techniques: Expressing, Staining, Confocal Laser Scanning Microscopy