NB110-60002 Search Results


resource source identifier antibodies defa5 human novus biologicals  (Novus Biologicals)
93
Novus Biologicals resource source identifier antibodies defa5 human novus biologicals
Resource Source Identifier Antibodies Defa5 Human Novus Biologicals, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/NB110-60002/Defensin+alpha+5+Antibody+(8C8)/pm41237766-658-2-8
Average 93 stars, based on 1 article reviews
resource source identifier antibodies defa5 human novus biologicals - by Bioz Stars, 2026-09
93/100 stars
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defensin a5 defa5 novus biologicals nb110 60002 mouse  (Novus Biologicals)
93
Novus Biologicals defensin a5 defa5 novus biologicals nb110 60002 mouse
Figure 6. Without EECs, intestinal stem and progenitor cells down-regulate master nutrient sensor mTORC1 in Paneth cells. (A) Immunofluorescence staining for phospho-S6 in crypts of wild-type and EEC-deficient human intestinal organoids. Defensin A5 <t>(DEFA5)</t> marks Paneth cells. 40,6-Diamidino-2-phenylindole (DAPI) counterstained nuclei in blue. Scale bar: 50 mm. Representative image of 5 organoids per genotype. (B) Immunofluorescence staining for phospho-S6 in small intestinal crypts of control, EEC-deficient, and fasted control mice. Ulex europaeus Agglutinin 1 (UEA-1) marks Paneth cells. DAPI counterstained nuclei in blue. Scale bar: 50 mm. Representative image of 6 control, 6 EEC-deficient, and 3 fasted control mice. (C) Higher magnification of crypt of control mouse in panel B. Arrows point to areas negative for phospho-S6 staining between Paneth cells. (D) Immunofluorescence staining for phospho-S6 in small intestinal crypts of Lgr5-eGFP-CreERT2 reporter mice. Arrows point to (eGFP)þ crypt–base columnar cells negative for phospho-S6 staining. Scale bar: 10 mm. Representative image of 3 mice. (E) Model depicting EEC regulation of crypt homeostasis and metabolism in response to nutrients (dashed arrow). In the fed condition, EECs (blue) activate mTORC1 signaling in Paneth cells (purple) and restrict intestinal stem cell activity, proliferation, and fatty acid oxidation (FAO) (dark green) (solid arrows). As progenitors (light green) move toward the villus and differentiate into absorptive enterocytes (light yellow), mitochondria undergoing FAO become abundant. eGFP, enhanced green fluorescent protein; prolif, proliferation.
Defensin A5 Defa5 Novus Biologicals Nb110 60002 Mouse, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/NB110-60002/Defensin+alpha+5+Antibody+(8C8)/pm36608902-248-65-68
Average 93 stars, based on 1 article reviews
defensin a5 defa5 novus biologicals nb110 60002 mouse - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

Image Search Results


Figure 6. Without EECs, intestinal stem and progenitor cells down-regulate master nutrient sensor mTORC1 in Paneth cells. (A) Immunofluorescence staining for phospho-S6 in crypts of wild-type and EEC-deficient human intestinal organoids. Defensin A5 (DEFA5) marks Paneth cells. 40,6-Diamidino-2-phenylindole (DAPI) counterstained nuclei in blue. Scale bar: 50 mm. Representative image of 5 organoids per genotype. (B) Immunofluorescence staining for phospho-S6 in small intestinal crypts of control, EEC-deficient, and fasted control mice. Ulex europaeus Agglutinin 1 (UEA-1) marks Paneth cells. DAPI counterstained nuclei in blue. Scale bar: 50 mm. Representative image of 6 control, 6 EEC-deficient, and 3 fasted control mice. (C) Higher magnification of crypt of control mouse in panel B. Arrows point to areas negative for phospho-S6 staining between Paneth cells. (D) Immunofluorescence staining for phospho-S6 in small intestinal crypts of Lgr5-eGFP-CreERT2 reporter mice. Arrows point to (eGFP)þ crypt–base columnar cells negative for phospho-S6 staining. Scale bar: 10 mm. Representative image of 3 mice. (E) Model depicting EEC regulation of crypt homeostasis and metabolism in response to nutrients (dashed arrow). In the fed condition, EECs (blue) activate mTORC1 signaling in Paneth cells (purple) and restrict intestinal stem cell activity, proliferation, and fatty acid oxidation (FAO) (dark green) (solid arrows). As progenitors (light green) move toward the villus and differentiate into absorptive enterocytes (light yellow), mitochondria undergoing FAO become abundant. eGFP, enhanced green fluorescent protein; prolif, proliferation.

Journal: Cellular and molecular gastroenterology and hepatology

Article Title: Enteroendocrine Cells Protect the Stem Cell Niche by Regulating Crypt Metabolism in Response to Nutrients.

doi: 10.1016/j.jcmgh.2022.12.016

Figure Lengend Snippet: Figure 6. Without EECs, intestinal stem and progenitor cells down-regulate master nutrient sensor mTORC1 in Paneth cells. (A) Immunofluorescence staining for phospho-S6 in crypts of wild-type and EEC-deficient human intestinal organoids. Defensin A5 (DEFA5) marks Paneth cells. 40,6-Diamidino-2-phenylindole (DAPI) counterstained nuclei in blue. Scale bar: 50 mm. Representative image of 5 organoids per genotype. (B) Immunofluorescence staining for phospho-S6 in small intestinal crypts of control, EEC-deficient, and fasted control mice. Ulex europaeus Agglutinin 1 (UEA-1) marks Paneth cells. DAPI counterstained nuclei in blue. Scale bar: 50 mm. Representative image of 6 control, 6 EEC-deficient, and 3 fasted control mice. (C) Higher magnification of crypt of control mouse in panel B. Arrows point to areas negative for phospho-S6 staining between Paneth cells. (D) Immunofluorescence staining for phospho-S6 in small intestinal crypts of Lgr5-eGFP-CreERT2 reporter mice. Arrows point to (eGFP)þ crypt–base columnar cells negative for phospho-S6 staining. Scale bar: 10 mm. Representative image of 3 mice. (E) Model depicting EEC regulation of crypt homeostasis and metabolism in response to nutrients (dashed arrow). In the fed condition, EECs (blue) activate mTORC1 signaling in Paneth cells (purple) and restrict intestinal stem cell activity, proliferation, and fatty acid oxidation (FAO) (dark green) (solid arrows). As progenitors (light green) move toward the villus and differentiate into absorptive enterocytes (light yellow), mitochondria undergoing FAO become abundant. eGFP, enhanced green fluorescent protein; prolif, proliferation.

Article Snippet: Images were acquired using a Nikon A1 GaAsP LUNV inverted confocal microscope and NIS Elements Table 1.Antibodies Used for Immunofluorescence Staining Antibody Company Catalog # Host Dilution Angiotensin Converting Enzyme 2 (Ace2) R&D Systems AF3437 Goat 1:200 Chromogranin A ImmunoStar 20085 Rabbit 1:200 Carbonic anhydrase 4 R&D Systems AF2414 Goat 1:200 Ki67 Cell Marque 275R-14 Rabbit 1:200 Cleaved caspase-3 Cell Signaling Technology 9661S Rabbit 1:200 Defensin A5 (DEFA5) Novus Biologicals NB110-60002 Mouse 1:60,000 Green Fluorescent Protein (GFP) Aves Labs GFP-1020 Chicken 1:200 Olfm4 (mouse specific) Cell Signaling Technology 39141S Rabbit 1:200 OLFM4 Cell Signaling Technology 14369T Rabbit 1:200 Phospho-S6 ribosomal protein (S235/236) Cell Signaling Technology 2211S Rabbit 1:200 UEA-1, DyLight 649 Vector Laboratories DL-1068 N/A 1:1000 UEA-1, fluorescein Invitrogen L32476 N/A 1:1000 UEA-1, Ulex europaeus Agglutinin 1. software (Nikon).

Techniques: Staining, Control, Activity Assay