NB100-77417 Search Results


rabbit polyclonal anti cd45  (Novus Biologicals)
93
Novus Biologicals rabbit polyclonal anti cd45
Rabbit Polyclonal Anti Cd45, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/NB100-77417/bio_rxiv__2025__06__22__660900-320-25-30?v=Novus+Biologicals
Average 93 stars, based on 1 article reviews
rabbit polyclonal anti cd45 - by Bioz Stars, 2026-08
93/100 stars
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cd45 ptprc  (Novus Biologicals)
94
Novus Biologicals cd45 ptprc
Figure 5. Marked increase in CR3 near the site of ONI. Complement C3b phagocytosis receptor CR3 is microglia/monocyte-specific, and CR31 microglia/monocyte number and phagocytic activation increase within the injured optic nerve. A, Representative images of CR3 immunolabeled uninjured (–) and injured (1, 3, 5, 7, 14 DPI) optic nerves at the injury site. Indicated regions (☐) are shown enlarged to the right of each image. Note the accumulation of CR31 cells and the increased cellular signal intensity following ONI. B, C, Quantitation confirms that the approximate number of CR31 cells (B) increases rapidly (by 1 DPI) and is sustained for at least 14 DPI, with a 30-fold peak at 5 DPI; likewise, CR3 immunofluorescence intensity (C) is elevated at all postinjury time points evaluated (1-14 DPI). D, The specificity of the CR3 (CD11b) antibody was verified using 14 DPI nerves from CR31/1 (left) and CR3/ mice (right). E, The approximate number of Iba11 microglia/monocytes in the nerve increases dramatically by 5 DPI and remains elevated until at least 14 DPI. F, Quantitation of CR31Iba1 (red), CR3-Iba11 (green), and colocalized CR31Iba11 (yellow) cells at/near the injury site. While most CR31 optic nerve cells are microglia, some CR31 cells in the injured nerve are likely infiltrating peripheral blood monocytes. G, CR3 (red) colocalization with Iba1 (green; microglia) at nerve injury site (3, 5 DPI), in the distal nerve (3 DPI), and in the chiasm (14 DPI). H–J, Presence of myeloid cell markers CD206 (H), <t>CD45</t> (I), and P2RY12 (J) in 14 DPI optic nerves. Iba11 and CR31 microglia/monocytes at/near the injury are large, round, and lack processes: morphologic changes associated with phagocytic activation. L, Similarly, these Iba11 (green) microglia/monocytes express strikingly high levels of lysosome marker CD68 (red; observed 3-14 DPI, 14 DPI shown). M, C1q deletion dampens the CR31 cell response to injury plus zymosan 1 CPT-cAMP treatment at 5 and 14 DPI. N, Pro-regenerative treatments may augment the CR31 cell response in the injured nerve at 14 DPI. Scale bars: A, 50 mm, 10 mm; D, G, H, I, J, L, 50 mm. Graphs represent either mean 6 SEM or 25th to 75th percentile (box), median (line), range (whiskers), and individual data points (circles). B, C, E, K, *p , 0.05, **p , 0.01, ***p , 0.001, ****p , 0.0001, one-way ANOVA with Dunnett post-test vs uninjured or untreated. J, **p , 0.01, t test.
Cd45 Ptprc, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/NB100-77417/10__1523_slash_jneurosci__0555___21__2021-110-62-70?v=Novus+Biologicals
Average 94 stars, based on 1 article reviews
cd45 ptprc - by Bioz Stars, 2026-08
94/100 stars
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anti cd45 mab  (Bio-Techne corporation)
96
Bio-Techne corporation anti cd45 mab
Figure 5. Marked increase in CR3 near the site of ONI. Complement C3b phagocytosis receptor CR3 is microglia/monocyte-specific, and CR31 microglia/monocyte number and phagocytic activation increase within the injured optic nerve. A, Representative images of CR3 immunolabeled uninjured (–) and injured (1, 3, 5, 7, 14 DPI) optic nerves at the injury site. Indicated regions (☐) are shown enlarged to the right of each image. Note the accumulation of CR31 cells and the increased cellular signal intensity following ONI. B, C, Quantitation confirms that the approximate number of CR31 cells (B) increases rapidly (by 1 DPI) and is sustained for at least 14 DPI, with a 30-fold peak at 5 DPI; likewise, CR3 immunofluorescence intensity (C) is elevated at all postinjury time points evaluated (1-14 DPI). D, The specificity of the CR3 (CD11b) antibody was verified using 14 DPI nerves from CR31/1 (left) and CR3/ mice (right). E, The approximate number of Iba11 microglia/monocytes in the nerve increases dramatically by 5 DPI and remains elevated until at least 14 DPI. F, Quantitation of CR31Iba1 (red), CR3-Iba11 (green), and colocalized CR31Iba11 (yellow) cells at/near the injury site. While most CR31 optic nerve cells are microglia, some CR31 cells in the injured nerve are likely infiltrating peripheral blood monocytes. G, CR3 (red) colocalization with Iba1 (green; microglia) at nerve injury site (3, 5 DPI), in the distal nerve (3 DPI), and in the chiasm (14 DPI). H–J, Presence of myeloid cell markers CD206 (H), <t>CD45</t> (I), and P2RY12 (J) in 14 DPI optic nerves. Iba11 and CR31 microglia/monocytes at/near the injury are large, round, and lack processes: morphologic changes associated with phagocytic activation. L, Similarly, these Iba11 (green) microglia/monocytes express strikingly high levels of lysosome marker CD68 (red; observed 3-14 DPI, 14 DPI shown). M, C1q deletion dampens the CR31 cell response to injury plus zymosan 1 CPT-cAMP treatment at 5 and 14 DPI. N, Pro-regenerative treatments may augment the CR31 cell response in the injured nerve at 14 DPI. Scale bars: A, 50 mm, 10 mm; D, G, H, I, J, L, 50 mm. Graphs represent either mean 6 SEM or 25th to 75th percentile (box), median (line), range (whiskers), and individual data points (circles). B, C, E, K, *p , 0.05, **p , 0.01, ***p , 0.001, ****p , 0.0001, one-way ANOVA with Dunnett post-test vs uninjured or untreated. J, **p , 0.01, t test.
Anti Cd45 Mab, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/NB100-77417/pmc07589468-200-22-35?v=Bio-Techne+corporation
Average 96 stars, based on 1 article reviews
anti cd45 mab - by Bioz Stars, 2026-08
96/100 stars
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Image Search Results


Figure 5. Marked increase in CR3 near the site of ONI. Complement C3b phagocytosis receptor CR3 is microglia/monocyte-specific, and CR31 microglia/monocyte number and phagocytic activation increase within the injured optic nerve. A, Representative images of CR3 immunolabeled uninjured (–) and injured (1, 3, 5, 7, 14 DPI) optic nerves at the injury site. Indicated regions (☐) are shown enlarged to the right of each image. Note the accumulation of CR31 cells and the increased cellular signal intensity following ONI. B, C, Quantitation confirms that the approximate number of CR31 cells (B) increases rapidly (by 1 DPI) and is sustained for at least 14 DPI, with a 30-fold peak at 5 DPI; likewise, CR3 immunofluorescence intensity (C) is elevated at all postinjury time points evaluated (1-14 DPI). D, The specificity of the CR3 (CD11b) antibody was verified using 14 DPI nerves from CR31/1 (left) and CR3/ mice (right). E, The approximate number of Iba11 microglia/monocytes in the nerve increases dramatically by 5 DPI and remains elevated until at least 14 DPI. F, Quantitation of CR31Iba1 (red), CR3-Iba11 (green), and colocalized CR31Iba11 (yellow) cells at/near the injury site. While most CR31 optic nerve cells are microglia, some CR31 cells in the injured nerve are likely infiltrating peripheral blood monocytes. G, CR3 (red) colocalization with Iba1 (green; microglia) at nerve injury site (3, 5 DPI), in the distal nerve (3 DPI), and in the chiasm (14 DPI). H–J, Presence of myeloid cell markers CD206 (H), CD45 (I), and P2RY12 (J) in 14 DPI optic nerves. Iba11 and CR31 microglia/monocytes at/near the injury are large, round, and lack processes: morphologic changes associated with phagocytic activation. L, Similarly, these Iba11 (green) microglia/monocytes express strikingly high levels of lysosome marker CD68 (red; observed 3-14 DPI, 14 DPI shown). M, C1q deletion dampens the CR31 cell response to injury plus zymosan 1 CPT-cAMP treatment at 5 and 14 DPI. N, Pro-regenerative treatments may augment the CR31 cell response in the injured nerve at 14 DPI. Scale bars: A, 50 mm, 10 mm; D, G, H, I, J, L, 50 mm. Graphs represent either mean 6 SEM or 25th to 75th percentile (box), median (line), range (whiskers), and individual data points (circles). B, C, E, K, *p , 0.05, **p , 0.01, ***p , 0.001, ****p , 0.0001, one-way ANOVA with Dunnett post-test vs uninjured or untreated. J, **p , 0.01, t test.

Journal: The Journal of Neuroscience

Article Title: Retinal Ganglion Cell Axon Regeneration Requires Complement and Myeloid Cell Activity within the Optic Nerve

doi: 10.1523/jneurosci.0555-21.2021

Figure Lengend Snippet: Figure 5. Marked increase in CR3 near the site of ONI. Complement C3b phagocytosis receptor CR3 is microglia/monocyte-specific, and CR31 microglia/monocyte number and phagocytic activation increase within the injured optic nerve. A, Representative images of CR3 immunolabeled uninjured (–) and injured (1, 3, 5, 7, 14 DPI) optic nerves at the injury site. Indicated regions (☐) are shown enlarged to the right of each image. Note the accumulation of CR31 cells and the increased cellular signal intensity following ONI. B, C, Quantitation confirms that the approximate number of CR31 cells (B) increases rapidly (by 1 DPI) and is sustained for at least 14 DPI, with a 30-fold peak at 5 DPI; likewise, CR3 immunofluorescence intensity (C) is elevated at all postinjury time points evaluated (1-14 DPI). D, The specificity of the CR3 (CD11b) antibody was verified using 14 DPI nerves from CR31/1 (left) and CR3/ mice (right). E, The approximate number of Iba11 microglia/monocytes in the nerve increases dramatically by 5 DPI and remains elevated until at least 14 DPI. F, Quantitation of CR31Iba1 (red), CR3-Iba11 (green), and colocalized CR31Iba11 (yellow) cells at/near the injury site. While most CR31 optic nerve cells are microglia, some CR31 cells in the injured nerve are likely infiltrating peripheral blood monocytes. G, CR3 (red) colocalization with Iba1 (green; microglia) at nerve injury site (3, 5 DPI), in the distal nerve (3 DPI), and in the chiasm (14 DPI). H–J, Presence of myeloid cell markers CD206 (H), CD45 (I), and P2RY12 (J) in 14 DPI optic nerves. Iba11 and CR31 microglia/monocytes at/near the injury are large, round, and lack processes: morphologic changes associated with phagocytic activation. L, Similarly, these Iba11 (green) microglia/monocytes express strikingly high levels of lysosome marker CD68 (red; observed 3-14 DPI, 14 DPI shown). M, C1q deletion dampens the CR31 cell response to injury plus zymosan 1 CPT-cAMP treatment at 5 and 14 DPI. N, Pro-regenerative treatments may augment the CR31 cell response in the injured nerve at 14 DPI. Scale bars: A, 50 mm, 10 mm; D, G, H, I, J, L, 50 mm. Graphs represent either mean 6 SEM or 25th to 75th percentile (box), median (line), range (whiskers), and individual data points (circles). B, C, E, K, *p , 0.05, **p , 0.01, ***p , 0.001, ****p , 0.0001, one-way ANOVA with Dunnett post-test vs uninjured or untreated. J, **p , 0.01, t test.

Article Snippet: Antibodies used in immunohistochemistry experiments Antibody Target (specificity) Host species Manufacturer Dilution C1q Complement C1q (C1q) Rabbit Abcam #ab182451 1:250 C3 Complement C3 (C3) Goat MP/Cappel #55730 1:1000 C3d Complement C3d (C3d opsonin) Rabbit Dako #A0063 1:1000 CC1 CC1, APC (mature oligodendrocytes) Mouse Abcam #ab16794 1:100 CD11b Complement receptor 3, CR3, Mac1, integrin aMb 2 (monocytes, microglia, PMNs) Rat Bio-Rad #MCA711G 1:1000 CD45 PTPRC (microglia, monocytes, PMNs, NKs, lymphocytes) Rat Novus Biologicals #NB100-77417 1:500 CD68 Lysosomes (phagocytes) Rat Bio-Rad #MCA1957 1:200 CD206 Mannose receptor (monocytes, microglia) Goat R&D Systems #RB01 1:200 GAP43 Growth-associated protein 43 (regenerating axons) Sheep Custom Variable GFAP Glial fibrillary acidic protein (astrocytes) Rabbit Dako #Z0334 1:5000 GFAP Glial fibrillary acidic protein (astrocytes) Mouse Sigma #G3893 1:2500 Iba1 Ionized calcium binding adaptor molecule 1 (microglia, monocytes) Rabbit Wako #01919741 1:500 MBP Myelin basic protein (myelin) Rabbit Abcam #ab40390 1:500 Olig2 Oligodendrocyte transcription factor 2 (oligodendrocytes) Goat R&D Systems #AF2418 1:500 P2Y12 Purinergic receptor P2Y (microglia) Rabbit AnaSpec #AS-55043A 1:500 TUJ1 b -III tubulin (RGCs) Rabbit Abcam #ab18207 1:500 mean RGC count for each retina was transformed to RGC survival density (RGCs/mm2, divided by 0.235 mm2 image area), then expressed as %WT control.

Techniques: Activation Assay, Immunolabeling, Quantitation Assay, Immunofluorescence, Marker