Journal: BMC Molecular and Cell Biology

Article Title: Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages

doi: 10.1186/s12860-020-00331-9

Figure Lengend Snippet: Antibodies used in the study

Article Snippet: CD45 (OX-1) , CD45, PTPRC , Novus Biologicals , NB100–64895 , 10 μg/ml.

Techniques: Concentration Assay, Flow Cytometry, Staining, FACS

Journal: BMC Molecular and Cell Biology

Article Title: Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages

doi: 10.1186/s12860-020-00331-9

Figure Lengend Snippet: Expression of the leukocyte marker PTPRC/CD45 in rat LSECs. a : Ptprc/Cd45 mRNA and PTPRC/CD45 protein expression as obtained from RNA-seq (log 2 RPKM) and label-free proteomics (log 2 iBAQ) in KCs (red dots) and LSECs (blue triangles). b-f : Representative sequential gating during flow cytometry analysis of rat non-parenchymal liver cells ( n = 4). Cells were labeled with antibodies to SE-1/FcγRIIb2, CD31, and CD45 (listed in Table ). b : Gating based on the FSC-A vs FSC-H profile to exclude duplets and aggregates from the subsequent analysis. c : Gating on the SSC-A vs FSC-A profile to select small cells with limited complexity (enriched in endothelial cells). d : Gating to select DAPI negative live cells. e-f : LSECs were then identified as SE-1-Alexa488 + cells ( e ), and the biexponential CD45-PE/CD31-APC of events ( f ) were used to display and select the CD45 + CD31 + subsets of LSECs. FMOs (used for gating), and single antibody staining controls are shown in Additional file . g : Average of the percentage of viable parent populations observed in 4 biological replicates (±standard deviation, SD)

Article Snippet: CD45 (OX-1) , CD45, PTPRC , Novus Biologicals , NB100–64895 , 10 μg/ml.

Techniques: Expressing, Marker, RNA Sequencing Assay, Flow Cytometry, Labeling, Staining, Standard Deviation

Journal: Advanced Functional Materials

Article Title: Biomimetic Nanoparticles as a Theranostic Tool for Traumatic Brain Injury

doi: 10.1002/adfm.202100722

Figure Lengend Snippet: Nanoparticles’ (NPs) properties characterization. After fabrication, NPs were characterized for their physicochemical and biological properties using dynamic light scattering. No significant changes in A) size and B) polydispersity index (PDI) were observed. However, a significant decrease in C) zeta potential for leukosomes (Leuko) compared to liposomes (Lipo) was noticed. D) Representative cryo‐TEM images of Lipo and Leuko verified that no morphological changes occurred following membrane protein integration to the NPs. Scale bars = 100 nm. E) Western blots for leukocyte membrane protein markers: CD11b, CD18, CD47, and CD45 indicated their membrane integration in Leuko but absence in Lipo. Results are shown as mean ± SEM. Unpaired t‐test was used to determine statistical probabilities * p ≤ 0.05 among means considered statistically significant, n = 5.

Article Snippet: Antibodies for western blot rat anti‐CD11b (MAB11241), goat anti‐CD18 (AF2618), rabbit anti‐CD45 (EPR20033), goat anti‐CD47 (ab108415), anti‐rabbit IgG‐HRP, anti‐goat IgG‐HRP, and anti‐rat IgG (Bio‐Techne Corporation, Minnesota, USA).

Techniques: Zeta Potential Analyzer, Liposomes, Membrane, Western Blot