NB100-1718 Search Results


anti ddx21  (Novus Biologicals)
94
Novus Biologicals anti ddx21
Anti Ddx21, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti ddx21/product/Novus Biologicals
Average 94 stars, based on 1 article reviews
anti ddx21 - by Bioz Stars, 2026-02
94/100 stars
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rabbit anti ddx21  (Novus Biologicals)
93
Novus Biologicals rabbit anti ddx21
a Comparison of nucleolar area between cycling cells and 5-day p16 arrested cells (>1000 cells for each condition). b Evaluation of transcriptional activity in G1-phase cycling cells or in 3-day arrested cells. Cells were pulsed with 5-Ethynyl Uridine (EU; 100 µM) for one hour prior to fixation and EU nuclear intensity measured by fluorescence imaging (>1000 cells for each condition). c Evaluation of transcriptional activity in cells arrested for 3 days in either full growth media, starvation media lacking serum and growth factors, or full growth media + Torin2 (100 nM). Cells were pulsed with 5-Ethynyl Uridine (EU; 100 µM) for one hour prior to fixation and EU nuclear intensity measured by fluorescence imaging (>1000 cells for each condition). d Representative immunofluorescence images of 3-day p16 arrested cells stained for 53BP1 to visualize DNA damage and nucleolin to visualize nucleoli. 53BP1 puncta are frequently observed in the perinucleolar area (arrows). e Manual quantification of the number of perinucleolar DNA damage puncta per cells in G1-phase cycling cells and 3-day arrested cells ( n = 250 cells per condition). f Three-day p16 arrested cells were fixed following a one-hour EU (100 µM) pulse and stained for γ-H2AX by immunofluorescence. Cells were binned into 5 groups by EU nuclear intensity and the average γ-H2AX intensity was calculated for each group of cells (bin 1, n = 1155; bin 2, n = 5252; bin 3, n = 3141; bin 4, n = 855; bin 5, n = 201). g Representative images of cycling cells and 3-day arrested cells stained for <t>Ddx21</t> by immunofluorescence. h quantification of the ratio of Ddx21 nucleolar fluorescence intensity to Ddx21 nucleoplasmic fluorescence intensity in G1 cycling cells and 3-day arrested cells treated with DMSO or with CX-5461 (5 µM) (>1000 cells for each condition). Kolmogorov–Smirnov test was used for analysis in ( a – c and h ). *** indicate p < 2.2 × 10 −16 .
Rabbit Anti Ddx21, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti ddx21/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
rabbit anti ddx21 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier

Image Search Results


a Comparison of nucleolar area between cycling cells and 5-day p16 arrested cells (>1000 cells for each condition). b Evaluation of transcriptional activity in G1-phase cycling cells or in 3-day arrested cells. Cells were pulsed with 5-Ethynyl Uridine (EU; 100 µM) for one hour prior to fixation and EU nuclear intensity measured by fluorescence imaging (>1000 cells for each condition). c Evaluation of transcriptional activity in cells arrested for 3 days in either full growth media, starvation media lacking serum and growth factors, or full growth media + Torin2 (100 nM). Cells were pulsed with 5-Ethynyl Uridine (EU; 100 µM) for one hour prior to fixation and EU nuclear intensity measured by fluorescence imaging (>1000 cells for each condition). d Representative immunofluorescence images of 3-day p16 arrested cells stained for 53BP1 to visualize DNA damage and nucleolin to visualize nucleoli. 53BP1 puncta are frequently observed in the perinucleolar area (arrows). e Manual quantification of the number of perinucleolar DNA damage puncta per cells in G1-phase cycling cells and 3-day arrested cells ( n = 250 cells per condition). f Three-day p16 arrested cells were fixed following a one-hour EU (100 µM) pulse and stained for γ-H2AX by immunofluorescence. Cells were binned into 5 groups by EU nuclear intensity and the average γ-H2AX intensity was calculated for each group of cells (bin 1, n = 1155; bin 2, n = 5252; bin 3, n = 3141; bin 4, n = 855; bin 5, n = 201). g Representative images of cycling cells and 3-day arrested cells stained for Ddx21 by immunofluorescence. h quantification of the ratio of Ddx21 nucleolar fluorescence intensity to Ddx21 nucleoplasmic fluorescence intensity in G1 cycling cells and 3-day arrested cells treated with DMSO or with CX-5461 (5 µM) (>1000 cells for each condition). Kolmogorov–Smirnov test was used for analysis in ( a – c and h ). *** indicate p < 2.2 × 10 −16 .

Journal: Nature Communications

Article Title: Uncoupling of mTORC1 from E2F activity maintains DNA damage and senescence

doi: 10.1038/s41467-024-52820-6

Figure Lengend Snippet: a Comparison of nucleolar area between cycling cells and 5-day p16 arrested cells (>1000 cells for each condition). b Evaluation of transcriptional activity in G1-phase cycling cells or in 3-day arrested cells. Cells were pulsed with 5-Ethynyl Uridine (EU; 100 µM) for one hour prior to fixation and EU nuclear intensity measured by fluorescence imaging (>1000 cells for each condition). c Evaluation of transcriptional activity in cells arrested for 3 days in either full growth media, starvation media lacking serum and growth factors, or full growth media + Torin2 (100 nM). Cells were pulsed with 5-Ethynyl Uridine (EU; 100 µM) for one hour prior to fixation and EU nuclear intensity measured by fluorescence imaging (>1000 cells for each condition). d Representative immunofluorescence images of 3-day p16 arrested cells stained for 53BP1 to visualize DNA damage and nucleolin to visualize nucleoli. 53BP1 puncta are frequently observed in the perinucleolar area (arrows). e Manual quantification of the number of perinucleolar DNA damage puncta per cells in G1-phase cycling cells and 3-day arrested cells ( n = 250 cells per condition). f Three-day p16 arrested cells were fixed following a one-hour EU (100 µM) pulse and stained for γ-H2AX by immunofluorescence. Cells were binned into 5 groups by EU nuclear intensity and the average γ-H2AX intensity was calculated for each group of cells (bin 1, n = 1155; bin 2, n = 5252; bin 3, n = 3141; bin 4, n = 855; bin 5, n = 201). g Representative images of cycling cells and 3-day arrested cells stained for Ddx21 by immunofluorescence. h quantification of the ratio of Ddx21 nucleolar fluorescence intensity to Ddx21 nucleoplasmic fluorescence intensity in G1 cycling cells and 3-day arrested cells treated with DMSO or with CX-5461 (5 µM) (>1000 cells for each condition). Kolmogorov–Smirnov test was used for analysis in ( a – c and h ). *** indicate p < 2.2 × 10 −16 .

Article Snippet: Primary antibodies used in this study were rabbit anti-phospho-H2AX (1:500, Cell Signaling Technologies #9718), rabbit anti-53BP1 (1:500, Cell Signaling Technologies #4937), rabbit anti-phospho-Rb (Ser807/811) (1:2500, Cell Signaling Technology #8516), rabbit anti-phospho-S6 Ribosomal Protein (1:500, Cell Signaling Technology #2215), rabbit anti-cGAS (1:500, Cell Signaling Technology #15102), rabbit phospho-Chk2 (Thr68) (1:500, Cell Signaling Technology # #2197), rabbit anti-cyclin D1 (1:500, Thermo-Fisher Scientific #MA5-14512), rabbit anti-Ddx21 (1:500, Novus Biologicals NB100-1718S), rabbit anti-p21 (1:2500, Cell Signaling Technology #2947), rabbit anti-p53 (1:1600, Cell Signaling Technology #2527).

Techniques: Comparison, Activity Assay, Fluorescence, Imaging, Immunofluorescence, Staining