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    New England Biolabs puc19 vector
    ( A ) Influence of ionic strength on <t>pUC19</t> condensation (400 ng) by OC3 and MC3 (25 μM) opioid compounds. Condensation reactions on pUC19 (400 ng) by opioid compounds in ( B ) acidic NaOAc buffer (80 mM, pH = 4.0), and ( C ) basic Tris buffer (80 mM, pH = 9.0) in the presence of 25 mM NaCl.
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    ( A ) Influence of ionic strength on pUC19 condensation (400 ng) by OC3 and MC3 (25 μM) opioid compounds. Condensation reactions on pUC19 (400 ng) by opioid compounds in ( B ) acidic NaOAc buffer (80 mM, pH = 4.0), and ( C ) basic Tris buffer (80 mM, pH = 9.0) in the presence of 25 mM NaCl.

    Journal: Nucleic Acids Research

    Article Title: C3-symmetric opioid scaffolds are pH-responsive DNA condensation agents

    doi: 10.1093/nar/gkw1097

    Figure Lengend Snippet: ( A ) Influence of ionic strength on pUC19 condensation (400 ng) by OC3 and MC3 (25 μM) opioid compounds. Condensation reactions on pUC19 (400 ng) by opioid compounds in ( B ) acidic NaOAc buffer (80 mM, pH = 4.0), and ( C ) basic Tris buffer (80 mM, pH = 9.0) in the presence of 25 mM NaCl.

    Article Snippet: AFM samples were prepared according to the following general procedure: in a total volume of 10 μl final concentrations of 3 ng/μl of pUC19 (NEB, N3041) or linearized pUC19, 5 mM MgCl and varying concentrations of test compound, were incubated at 37°C for 1 h. Ten microliters of each sample was pipetted directly on to freshly cleaved mica and allowed to incubate for 5 min followed by rinsing with 500 μl of water.

    Techniques:

    ( A ) Agarose gel electrophoresis of supercoiled (400 ng) and ( B ) a 742 bp dsDNA fragment of pUC19 (400 ng) exposed to increasing concentrations of MC3, OC3 and HC3 . Reactions were carried out in the presence of 25 mM NaCl for 5 h at 37°C prior to electrophoretic analysis.

    Journal: Nucleic Acids Research

    Article Title: C3-symmetric opioid scaffolds are pH-responsive DNA condensation agents

    doi: 10.1093/nar/gkw1097

    Figure Lengend Snippet: ( A ) Agarose gel electrophoresis of supercoiled (400 ng) and ( B ) a 742 bp dsDNA fragment of pUC19 (400 ng) exposed to increasing concentrations of MC3, OC3 and HC3 . Reactions were carried out in the presence of 25 mM NaCl for 5 h at 37°C prior to electrophoretic analysis.

    Article Snippet: AFM samples were prepared according to the following general procedure: in a total volume of 10 μl final concentrations of 3 ng/μl of pUC19 (NEB, N3041) or linearized pUC19, 5 mM MgCl and varying concentrations of test compound, were incubated at 37°C for 1 h. Ten microliters of each sample was pipetted directly on to freshly cleaved mica and allowed to incubate for 5 min followed by rinsing with 500 μl of water.

    Techniques: Agarose Gel Electrophoresis

    Atomic force microscopy (AFM) images of MC3 -treated supercoiled and HindIII linearized pUC19 DNA; ( A–D ) supercoiled pUC19 with 8, 9, 10 and 20 μM MC3 ; ( E–H ) linear pUC19 with 5, 10, 20 and 50 μM MC3 .

    Journal: Nucleic Acids Research

    Article Title: C3-symmetric opioid scaffolds are pH-responsive DNA condensation agents

    doi: 10.1093/nar/gkw1097

    Figure Lengend Snippet: Atomic force microscopy (AFM) images of MC3 -treated supercoiled and HindIII linearized pUC19 DNA; ( A–D ) supercoiled pUC19 with 8, 9, 10 and 20 μM MC3 ; ( E–H ) linear pUC19 with 5, 10, 20 and 50 μM MC3 .

    Article Snippet: AFM samples were prepared according to the following general procedure: in a total volume of 10 μl final concentrations of 3 ng/μl of pUC19 (NEB, N3041) or linearized pUC19, 5 mM MgCl and varying concentrations of test compound, were incubated at 37°C for 1 h. Ten microliters of each sample was pipetted directly on to freshly cleaved mica and allowed to incubate for 5 min followed by rinsing with 500 μl of water.

    Techniques: Microscopy