Journal: Nucleic Acids Research
Article Title: C3-symmetric opioid scaffolds are pH-responsive DNA condensation agents
Figure Lengend Snippet: ( A ) Agarose gel electrophoresis of supercoiled (400 ng) and ( B ) a 742 bp dsDNA fragment of pUC19 (400 ng) exposed to increasing concentrations of MC3, OC3 and HC3 . Reactions were carried out in the presence of 25 mM NaCl for 5 h at 37°C prior to electrophoretic analysis.
Article Snippet: AFM samples were prepared according to the following general procedure: in a total volume of 10 μl final concentrations of 3 ng/μl of pUC19 (NEB, N3041) or linearized pUC19, 5 mM MgCl and varying concentrations of test compound, were incubated at 37°C for 1 h. Ten microliters of each sample was pipetted directly on to freshly cleaved mica and allowed to incubate for 5 min followed by rinsing with 500 μl of water.
Techniques: Agarose Gel Electrophoresis