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    • yeast strain sc252a  (ATCC)
    90
    ATCC yeast strain sc252a
    (A) Schematic of the dicistronic DNA construct. The yeCherry gene is in the 5’-position driven by the 700-nt ADH1 promoter. The “X” represents one of four regulatory elements (see Figure 1) to enhance expression of the 3’-gene yeGFP. The ADH1 termination sequence follows the yeGFP gene. (B)-(E) Fluorescence micrographs of <t>SC252a</t> cells that harbor vectors carrying one of the four dicistronic constructs. In all cases yeCherry is the 5’-gene driven by the 700-nt ADH1 promoter. The yeGFP gene is driven by the (B) pTEF1 promoter from Ashbya gossypii, (C) pTEF1 promoter from Saccharomyces cerevisiae, (D) p150 IRES or (E) YAP1 IRES. All exposures are 0.5 seconds.
    Yeast Strain Sc252a, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/yeast strain sc252a/product/ATCC
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    yeast strain sc252a - by Bioz Stars, 2024-10
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    (A) Schematic of the dicistronic DNA construct. The yeCherry gene is in the 5’-position driven by the 700-nt ADH1 promoter. The “X” represents one of four regulatory elements (see Figure 1) to enhance expression of the 3’-gene yeGFP. The ADH1 termination sequence follows the yeGFP gene. (B)-(E) Fluorescence micrographs of SC252a cells that harbor vectors carrying one of the four dicistronic constructs. In all cases yeCherry is the 5’-gene driven by the 700-nt ADH1 promoter. The yeGFP gene is driven by the (B) pTEF1 promoter from Ashbya gossypii, (C) pTEF1 promoter from Saccharomyces cerevisiae, (D) p150 IRES or (E) YAP1 IRES. All exposures are 0.5 seconds.

    Journal: Yeast (Chichester, England)

    Article Title: Dicistronic regulation of fluorescent proteins in the budding yeast Saccharomyces cerevisiae

    doi: 10.1002/yea.1744

    Figure Lengend Snippet: (A) Schematic of the dicistronic DNA construct. The yeCherry gene is in the 5’-position driven by the 700-nt ADH1 promoter. The “X” represents one of four regulatory elements (see Figure 1) to enhance expression of the 3’-gene yeGFP. The ADH1 termination sequence follows the yeGFP gene. (B)-(E) Fluorescence micrographs of SC252a cells that harbor vectors carrying one of the four dicistronic constructs. In all cases yeCherry is the 5’-gene driven by the 700-nt ADH1 promoter. The yeGFP gene is driven by the (B) pTEF1 promoter from Ashbya gossypii, (C) pTEF1 promoter from Saccharomyces cerevisiae, (D) p150 IRES or (E) YAP1 IRES. All exposures are 0.5 seconds.

    Article Snippet: The yeast strain SC252a (ATCC, catalog number: MYA-5513) was used for all experiments.

    Techniques: Construct, Expressing, Sequencing, Fluorescence