MAB5259 Search Results


94
R&D Systems anti ifn λ3
Anti Ifn λ3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/MAB5259/Human+IL-28B%2FIFN-lambda+3+Antibody/pmc05442324-254-26-27
Average 94 stars, based on 1 article reviews
anti ifn λ3 - by Bioz Stars, 2026-11
94/100 stars
  Buy from Supplier

94
R&D Systems ifn λ3
Measurements from Calu-3 cells at 24 hours after either PR8 IAV MOI 1 infection or mock infection: (A) <t>IFN-λ3</t> in cell lysate by Western blot, (B) parkin in cell lysate by Western blot, (C) green fluorescent protein IAV–positive (GFP-IAV–positive) cells, (D) total GFP-IAV fluorescence, (E) cellular apoptosis initiation measured by caspase-3/7 activity, and (F) cells displaying necrotic/late apoptotic features, as measured by double positivity with annexin V and cytotoxicity dyes. Statistical differences were determined by unpaired 2-tailed Student’s t test (A–C). The violin plots are closed curves representing data distribution and encapsulate the median, range, and interquartilerange, with each symbol representing a biological replicate. Data are representative of at least 2 independent experiments with n = 6 biological replicates/group (A and B) or n = 4 biological replicates/group (C–F). *P < 0.05, **P < 0.01. Statistical differences were determined by unpaired 2-tailed Student’s t test.
Ifn λ3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/MAB5259/Human+IL-28B%2FIFN-lambda+3+Antibody/pmc06629144-496-19-20
Average 94 stars, based on 1 article reviews
ifn λ3 - by Bioz Stars, 2026-11
94/100 stars
  Buy from Supplier

Image Search Results


Measurements from Calu-3 cells at 24 hours after either PR8 IAV MOI 1 infection or mock infection: (A) IFN-λ3 in cell lysate by Western blot, (B) parkin in cell lysate by Western blot, (C) green fluorescent protein IAV–positive (GFP-IAV–positive) cells, (D) total GFP-IAV fluorescence, (E) cellular apoptosis initiation measured by caspase-3/7 activity, and (F) cells displaying necrotic/late apoptotic features, as measured by double positivity with annexin V and cytotoxicity dyes. Statistical differences were determined by unpaired 2-tailed Student’s t test (A–C). The violin plots are closed curves representing data distribution and encapsulate the median, range, and interquartilerange, with each symbol representing a biological replicate. Data are representative of at least 2 independent experiments with n = 6 biological replicates/group (A and B) or n = 4 biological replicates/group (C–F). *P < 0.05, **P < 0.01. Statistical differences were determined by unpaired 2-tailed Student’s t test.

Journal: JCI Insight

Article Title: Macrophage migration inhibitory factor enhances influenza-associated mortality in mice

doi: 10.1172/jci.insight.128034

Figure Lengend Snippet: Measurements from Calu-3 cells at 24 hours after either PR8 IAV MOI 1 infection or mock infection: (A) IFN-λ3 in cell lysate by Western blot, (B) parkin in cell lysate by Western blot, (C) green fluorescent protein IAV–positive (GFP-IAV–positive) cells, (D) total GFP-IAV fluorescence, (E) cellular apoptosis initiation measured by caspase-3/7 activity, and (F) cells displaying necrotic/late apoptotic features, as measured by double positivity with annexin V and cytotoxicity dyes. Statistical differences were determined by unpaired 2-tailed Student’s t test (A–C). The violin plots are closed curves representing data distribution and encapsulate the median, range, and interquartilerange, with each symbol representing a biological replicate. Data are representative of at least 2 independent experiments with n = 6 biological replicates/group (A and B) or n = 4 biological replicates/group (C–F). *P < 0.05, **P < 0.01. Statistical differences were determined by unpaired 2-tailed Student’s t test.

Article Snippet: Membranes were then incubated for 1 hour at room temperature with primary antibodies against parkin (Abcam, catalog ab77924; 1:500), IFN-λ3 (R&D Systems, catalog MAB5259), β-actin (Abcam, catalog ab8226; 1:1000).

Techniques: Infection, Western Blot, Fluorescence, Activity Assay