MAB105401 Search Results


mouse anti rbd  (R&D Systems)
93
R&D Systems mouse anti rbd
Mouse Anti Rbd, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti rbd/product/R&D Systems
Average 93 stars, based on 1 article reviews
mouse anti rbd - by Bioz Stars, 2026-04
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rbd  (R&D Systems)
92
R&D Systems rbd
Assays for testing the functionality of SARS-CoV2 spike protein produced in Chlamydomonas. (a) In vitro pull-down assay with full-length spike protein from a S-GSAS/PP transformant and <t>recombinant</t> <t>hACE2.</t> Proteins from the culture medium (CM) of a S-GSAS/PP transformant that had been precipitated with ammonium sulfate and dialyzed against PBS were incubated in the presence or absence of recombinant hACE2 containing a deca-histidine tag. Nickel resin was added, sedimented by centrifugation and the supernatant (S) collected. The resin was washed once (W), and bound proteins eluted with imidazole (E). Proteins in S, W and E were precipitated with TCA (100%) and analyzed next to 10% of the input (In) by immunoblotting using antibodies against hACE2 and the HA epitope. Shown is one representative experiment out of three with similar outcome. (b) In vitro pull-down assay with <t>RBD-sfGFP</t> and recombinant hACE2. The experiment was conducted as in (a) but using human derived RBD-sfGFP concentrated with centrifugal filters. (c) Cell-based functionality assay with full-length spike protein from a S-GSAS/PP transformant and RBD-sfGFP. Proteins in culture medium from a S-GSAS/PP transformant and from wild type were concentrated by ammonium sulfate precipitation, dialyzed against PBS, and applied to 293T cells constitutively overexpressing h A CE2 and h T MPRSS2 (293T+AT) and regular 293T cells. As control, RBD-sfGFP concentrated with centrifugal filters was applied. Cells were washed, lysed and total proteins subjected to SDS-PAGE and immunoblot analysis using antibodies against the HA epitope, ACE2, and the RBD. GAPDH was detected as endogenous housekeeping control. Shown is one representative experiment out of four with similar outcome.
Rbd, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rbd/product/R&D Systems
Average 92 stars, based on 1 article reviews
rbd - by Bioz Stars, 2026-04
92/100 stars
  Buy from Supplier

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Assays for testing the functionality of SARS-CoV2 spike protein produced in Chlamydomonas. (a) In vitro pull-down assay with full-length spike protein from a S-GSAS/PP transformant and recombinant hACE2. Proteins from the culture medium (CM) of a S-GSAS/PP transformant that had been precipitated with ammonium sulfate and dialyzed against PBS were incubated in the presence or absence of recombinant hACE2 containing a deca-histidine tag. Nickel resin was added, sedimented by centrifugation and the supernatant (S) collected. The resin was washed once (W), and bound proteins eluted with imidazole (E). Proteins in S, W and E were precipitated with TCA (100%) and analyzed next to 10% of the input (In) by immunoblotting using antibodies against hACE2 and the HA epitope. Shown is one representative experiment out of three with similar outcome. (b) In vitro pull-down assay with RBD-sfGFP and recombinant hACE2. The experiment was conducted as in (a) but using human derived RBD-sfGFP concentrated with centrifugal filters. (c) Cell-based functionality assay with full-length spike protein from a S-GSAS/PP transformant and RBD-sfGFP. Proteins in culture medium from a S-GSAS/PP transformant and from wild type were concentrated by ammonium sulfate precipitation, dialyzed against PBS, and applied to 293T cells constitutively overexpressing h A CE2 and h T MPRSS2 (293T+AT) and regular 293T cells. As control, RBD-sfGFP concentrated with centrifugal filters was applied. Cells were washed, lysed and total proteins subjected to SDS-PAGE and immunoblot analysis using antibodies against the HA epitope, ACE2, and the RBD. GAPDH was detected as endogenous housekeeping control. Shown is one representative experiment out of four with similar outcome.

Journal: bioRxiv

Article Title: Production and secretion of functional full-length SARS-CoV-2 spike protein in Chlamydomonas reinhardtii

doi: 10.1101/2021.12.13.472433

Figure Lengend Snippet: Assays for testing the functionality of SARS-CoV2 spike protein produced in Chlamydomonas. (a) In vitro pull-down assay with full-length spike protein from a S-GSAS/PP transformant and recombinant hACE2. Proteins from the culture medium (CM) of a S-GSAS/PP transformant that had been precipitated with ammonium sulfate and dialyzed against PBS were incubated in the presence or absence of recombinant hACE2 containing a deca-histidine tag. Nickel resin was added, sedimented by centrifugation and the supernatant (S) collected. The resin was washed once (W), and bound proteins eluted with imidazole (E). Proteins in S, W and E were precipitated with TCA (100%) and analyzed next to 10% of the input (In) by immunoblotting using antibodies against hACE2 and the HA epitope. Shown is one representative experiment out of three with similar outcome. (b) In vitro pull-down assay with RBD-sfGFP and recombinant hACE2. The experiment was conducted as in (a) but using human derived RBD-sfGFP concentrated with centrifugal filters. (c) Cell-based functionality assay with full-length spike protein from a S-GSAS/PP transformant and RBD-sfGFP. Proteins in culture medium from a S-GSAS/PP transformant and from wild type were concentrated by ammonium sulfate precipitation, dialyzed against PBS, and applied to 293T cells constitutively overexpressing h A CE2 and h T MPRSS2 (293T+AT) and regular 293T cells. As control, RBD-sfGFP concentrated with centrifugal filters was applied. Cells were washed, lysed and total proteins subjected to SDS-PAGE and immunoblot analysis using antibodies against the HA epitope, ACE2, and the RBD. GAPDH was detected as endogenous housekeeping control. Shown is one representative experiment out of four with similar outcome.

Article Snippet: The membranes were blocked and incubated with specific antibodies: HA-tagged spike protein was detected with anti-HA antibody (H9658, Sigma-Aldrich, 1:10,000), hACE2 was detected with anti-ACE2 antibody (sc-390851, Santa Cruz Biotechnology, 1:2,000), RBD was detected using anti-RBD antibody (MAB105401, R&D Systems, 1:2,000). m-IgGκ BP-HRP was used as secondary antibody for detection (sc-516102, Santa Cruz Biotechnology, 1:10,000).

Techniques: Produced, In Vitro, Pull Down Assay, Recombinant, Incubation, Centrifugation, Western Blot, Derivative Assay, SDS Page