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    • m0240s  (New England Biolabs)
    99
    New England Biolabs m0240s
    A) Fluorescence emission of 4 μM probe OGR1 in <t>Fpg</t> reaction buffer at 37 °C without Fpg (gray) and after 25 min with Fpg (black). B) Time course of 4 μM probe OGR1 emission at 465 nm following addition of 658 nM Fpg (black triangles) or <t>hOGG1</t> (gray circles) at 37 °C. This is a representative curve of n=1.
    M0240s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A) Fluorescence emission of 4 μM probe OGR1 in Fpg reaction buffer at 37 °C without Fpg (gray) and after 25 min with Fpg (black). B) Time course of 4 μM probe OGR1 emission at 465 nm following addition of 658 nM Fpg (black triangles) or hOGG1 (gray circles) at 37 °C. This is a representative curve of n=1.

    Journal: Chembiochem : a European journal of chemical biology

    Article Title: In Vitro Fluorogenic Real-time Assay of the Repair of Oxidative DNA Damage

    doi: 10.1002/cbic.201500184

    Figure Lengend Snippet: A) Fluorescence emission of 4 μM probe OGR1 in Fpg reaction buffer at 37 °C without Fpg (gray) and after 25 min with Fpg (black). B) Time course of 4 μM probe OGR1 emission at 465 nm following addition of 658 nM Fpg (black triangles) or hOGG1 (gray circles) at 37 °C. This is a representative curve of n=1.

    Article Snippet: Fpg, hOGG1, Fpg reaction buffer (NEB buffer 1: 10 mM Bis-Tris-Propane-HCl, 10 mM MgCl2 , 1 mM dithiothreitol, pH 7.0 at 25 °C), hOGG1 reaction buffer (NEB buffer 4: 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, 1 mM dithiothreitol, pH 7.9 at 25 °C), and BSA (10 mg/mL) were purchased from New England Biolabs.

    Techniques: Fluorescence

    A and B) PAGE analysis of 4 μM probe OGR1 radiolabeled at the 5′ end with [ 32 P]-phosphate and reacted with 658 nM hOGG1 (A) or Fpg (B) at 37 °C for various times. Piperidine (200 mM, 95 °C, 5 min), which cleaves the DNA backbone at abasic sites, was added to samples as indicated to identify the 8-oxoguanine excision product and the backbone cleavage product. C and D) MALDI mass spectrometry analysis of probe OGR1 following overnight reaction with hOGG1 (C) or Fpg (D).

    Journal: Chembiochem : a European journal of chemical biology

    Article Title: In Vitro Fluorogenic Real-time Assay of the Repair of Oxidative DNA Damage

    doi: 10.1002/cbic.201500184

    Figure Lengend Snippet: A and B) PAGE analysis of 4 μM probe OGR1 radiolabeled at the 5′ end with [ 32 P]-phosphate and reacted with 658 nM hOGG1 (A) or Fpg (B) at 37 °C for various times. Piperidine (200 mM, 95 °C, 5 min), which cleaves the DNA backbone at abasic sites, was added to samples as indicated to identify the 8-oxoguanine excision product and the backbone cleavage product. C and D) MALDI mass spectrometry analysis of probe OGR1 following overnight reaction with hOGG1 (C) or Fpg (D).

    Article Snippet: Fpg, hOGG1, Fpg reaction buffer (NEB buffer 1: 10 mM Bis-Tris-Propane-HCl, 10 mM MgCl2 , 1 mM dithiothreitol, pH 7.0 at 25 °C), hOGG1 reaction buffer (NEB buffer 4: 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, 1 mM dithiothreitol, pH 7.9 at 25 °C), and BSA (10 mg/mL) were purchased from New England Biolabs.

    Techniques: Polyacrylamide Gel Electrophoresis, Mass Spectrometry