Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

Article Title: Protection of Anthocyanin from Myrica rubra against Cerebral Ischemia-Reperfusion Injury via Modulation of the TLR4/NF-κB and NLRP3 Pathways

doi: 10.3390/molecules23071788

Figure Lengend Snippet: Effect of PAEs from Dongkui Myrica rubra on protein expression: ( A ) the expressions of Toll-like receptor 4 (TLR4), tumor necrosis factor alpha (TNF-α), and β-actin; ( B ) the expressions of nuclear erythroid 2-related factor 2 (Nrf2), caspase-1, NOD-like receptor pyrin domain-containing 3 protein (NLRP3), IL-18, and β-actin. The quantifications of ( A ) and ( B ) are shown in ( C – H ). All of the data are shown as mean ± SD ( n = 10); * p < 0.05, ** p < 0.01 vs. I/R group. ( I ) The signal pathways; ↑ represents activation, while T (red line) represents inhibition.

Article Snippet: The primary-antibody dilutions were anri-TLR4 (1:500), anti-tumor necrosis factor alpha (TNF-α; 1:500), anti- nuclear erythroid 2-related factor 2 (Nrf2; 1:1000), anti-caspase-1 (1:1000), anti-NLRP3 (1:1000), and IL-18 (1:1000) (Boster Biological Technology Ltd., Wuhan, China).

Techniques: Expressing, Activation Assay, Inhibition