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anti s100a9  (Boster Bio)
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A. KEGG pathway analysis based on differentially upregulated expressed genes in Stx12 −/− mice (-log 10 (padj) > 5.5) from RNA-seq data. B. The number of neutrophil in the blood from Stx12 −/− and the littermate control (WT, Stx12 +/+ mice. C. The relative mRNA level of neutrophil markers (Ly6g, MPO, ELANE, and CSF3R) and key chemokines for neutrophil recruitment and activation (CSF3, C3, CXCL1, CXCL2, CXCL5, and TNF) normalized to β-actin. WT n = 5, HO n = 5 independent samples. D. IL-1β levels in the lung lysate from Stx12 −/− (n = 8) and the littermate control (WT) mice (n = 6) by enzyme-linked immunosorbent assay. E. Relative mRNA levels of IL-6 from lung of Stx12 −/− (HO) and the littermate control (WT, Stx12 +/+ ) mice. F. The expression of IL-6, <t>S100A9</t> and GAPDH by western blot in lung lysate from Stx12 −/− (HO) and the littermate control (WT, Stx12 +/+ ) mice. G. Quantification of the levels of IL-6 and S100A9 normalized to GAPDH. H. Heatmap showing upregulated inflammation-related gene including neutrophil-related gene, interleukin, tumor necrosis factor (TNF)-related gene, complement(C) - related gene, colony stimulating factor (CSF)-related gene and chemokines in WT and Stx12 −/− lung tissue. I. The picture of newborn mice after drug intervention. Results are presented as mean ± SEM; statistical significance was assessed by Student’s t-test.
Anti S100a9, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A. KEGG pathway analysis based on differentially upregulated expressed genes in Stx12 −/− mice (-log 10 (padj) > 5.5) from RNA-seq data. B. The number of neutrophil in the blood from Stx12 −/− and the littermate control (WT, Stx12 +/+ mice. C. The relative mRNA level of neutrophil markers (Ly6g, MPO, ELANE, and CSF3R) and key chemokines for neutrophil recruitment and activation (CSF3, C3, CXCL1, CXCL2, CXCL5, and TNF) normalized to β-actin. WT n = 5, HO n = 5 independent samples. D. IL-1β levels in the lung lysate from Stx12 −/− (n = 8) and the littermate control (WT) mice (n = 6) by enzyme-linked immunosorbent assay. E. Relative mRNA levels of IL-6 from lung of Stx12 −/− (HO) and the littermate control (WT, Stx12 +/+ ) mice. F. The expression of IL-6, S100A9 and GAPDH by western blot in lung lysate from Stx12 −/− (HO) and the littermate control (WT, Stx12 +/+ ) mice. G. Quantification of the levels of IL-6 and S100A9 normalized to GAPDH. H. Heatmap showing upregulated inflammation-related gene including neutrophil-related gene, interleukin, tumor necrosis factor (TNF)-related gene, complement(C) - related gene, colony stimulating factor (CSF)-related gene and chemokines in WT and Stx12 −/− lung tissue. I. The picture of newborn mice after drug intervention. Results are presented as mean ± SEM; statistical significance was assessed by Student’s t-test.

Journal: bioRxiv

Article Title: Pulmonary Mitochondrial DNA Release and Activation of the cGAS-STING Pathway in Lethal Stx12 Knockout Mice

doi: 10.1101/2024.10.07.616980

Figure Lengend Snippet: A. KEGG pathway analysis based on differentially upregulated expressed genes in Stx12 −/− mice (-log 10 (padj) > 5.5) from RNA-seq data. B. The number of neutrophil in the blood from Stx12 −/− and the littermate control (WT, Stx12 +/+ mice. C. The relative mRNA level of neutrophil markers (Ly6g, MPO, ELANE, and CSF3R) and key chemokines for neutrophil recruitment and activation (CSF3, C3, CXCL1, CXCL2, CXCL5, and TNF) normalized to β-actin. WT n = 5, HO n = 5 independent samples. D. IL-1β levels in the lung lysate from Stx12 −/− (n = 8) and the littermate control (WT) mice (n = 6) by enzyme-linked immunosorbent assay. E. Relative mRNA levels of IL-6 from lung of Stx12 −/− (HO) and the littermate control (WT, Stx12 +/+ ) mice. F. The expression of IL-6, S100A9 and GAPDH by western blot in lung lysate from Stx12 −/− (HO) and the littermate control (WT, Stx12 +/+ ) mice. G. Quantification of the levels of IL-6 and S100A9 normalized to GAPDH. H. Heatmap showing upregulated inflammation-related gene including neutrophil-related gene, interleukin, tumor necrosis factor (TNF)-related gene, complement(C) - related gene, colony stimulating factor (CSF)-related gene and chemokines in WT and Stx12 −/− lung tissue. I. The picture of newborn mice after drug intervention. Results are presented as mean ± SEM; statistical significance was assessed by Student’s t-test.

Article Snippet: The primary antibodies used included the following: rabbit anti-STX12 monoclonal antibody (1:1000, Custom-made), mouse anti-β-actin (1:5000; Proteintech, #60008-1-Ig), mouse anti-GAPDH (1:20000, Proteintech, #60004-1-Ig), anti-OXPHOS antibody (1:1000; Abcam, #ab110413), anti-cGAS (1:1000; Cell Signaling Technology, #31659), anti-TBK1(1:1000, Cell Signaling Technology, #3504S), anti-pTBK1(1:1000; Cell Signaling Technology, # 5483S), anti-IRF3(1:1000; Proteintech, #11312-1-AP), anti-pIRF3(1:1000; Cell Signaling Technology, #29047), anti-IL-6 (1:1000; Abcam, #ab229381), anti-S100A9 (1:1000; Boster, #PB0718).

Techniques: RNA Sequencing Assay, Control, Activation Assay, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot