Journal: Cancer research
Article Title: Loss of FOXO1 cooperates with TMPRSS2-ERG overexpression to promote prostate tumorigenesis and cell invasion
Figure Lengend Snippet: FOXO1 interacts directly with ERG. A, Western blot analysis of endogenous FOXO1 and ERG proteins in VCaP cells immunoprecipitated by indicated antibody or non-specific IgG. B, Western blot analysis of ectopically expressed proteins immunoprecipitated by indicated antibody or non-specific IgG in PC-3 cells transfected with indicated Flag- and Myc-tagged plasmids. C , Schematic depicting a set of GST-ERG recombinant protein constructs. D, Coomassie Blue staining of GST and GST-ERG recombinant proteins purified from bacteria (bottom), and Western blot analysis of FOXO1 proteins in LAPC-4 cells pulled down by GST recombinant proteins (top). Red arrow, non-specific band. * in red, unexpected, but reproducible band shift. * in black, denotes expected fragment size. E, Schematic depicting a set of GST-FOXO1 recombinant protein constructs. F, GelCode Blue staining of GST and GST-FOXO1 recombinant proteins purified from bacteria (bottom), and Western blot analysis of in vitro transcribed and translated ERG proteins pulled down by GST recombinant proteins (top).
Article Snippet: The cell lines VCaP, PC-3, and LAPC-4 were purchased from ATCC (Manassas, VA) and authenticated via STR profiling.
Techniques: Western Blot, Immunoprecipitation, Transfection, Recombinant, Construct, Staining, Purification, Electrophoretic Mobility Shift Assay, In Vitro