Journal: Prostate cancer and prostatic diseases

Article Title: A Phase II Trial of a Combination Herbal Supplement for Men with Biochemically Recurrent Prostate Cancer

doi: 10.1038/pcan.2014.37

Figure Lengend Snippet: Human prostate cancer LNCaP, PC3 and LAPC3 cells were seeded into 96-well plates (3000 cells/well) to grow to 80% confluency. The dry PHC powder was dissolved in serum-free RPMI 1640 (SF-RPMI) and added to the cells at the final concentrations of 0 (SF-RPMI as the vehicle control), 0.01, 0.1 and 1 mg/ml, respectively. Following 72 hour incubation at 37°C in a 5% CO2 atmosphere, the cells were washed and subjected to the MTS assay. The absorbance was detected at 490 nm with a Microplate Reader. Optical density (OD) decreased in a dose-dependent manner, representing suppression of proliferation (A). AR expression was measured via Western Blot and also showed that PHC reduced expression in a dose-dependent pattern (B). βactin is used as control, representing protein expression of a “housekeeping” gene.

Article Snippet: Human prostate cancer LNCaP, PC3 and LAPC3 cells (American Type Culture Collection, Manassas, VA) were cultured and maintained at 37°C in a 5% CO2 atmosphere in RPMI-1640 medium supplemented with 5% fetal bovine serum, 100 units/mL penicillin and 100 μg/mL streptomycin.

Techniques: Control, Incubation, MTS Assay, Expressing, Western Blot

Journal: Prostate cancer and prostatic diseases

Article Title: A Phase II Trial of a Combination Herbal Supplement for Men with Biochemically Recurrent Prostate Cancer

doi: 10.1038/pcan.2014.37

Figure Lengend Snippet: Three cells lines, LNCaP, LAPC3 and 22RV1, of prostate cancer were cultured on cover slips and treated with PHC (10 μg/ml) for 4 days. The PHC-induced apoptosis was examined by identifying DNA fragmentation with fluorescent TUNEL assay and counter-staining with DAPI. All apoptotic (TUNEL-positive) cell nuclei had bright green fluorescence under a fluorescence microscope. The result was expressed as an apoptotic index defined as the mean of TUNEL-positive cells counted per 400x field. P<0.05 indicated significant difference. Representative photomicrographs demonstrated the DNA fragmentation and dysmorphic nuclear condensation , which were significantly increased in response to PHC treatment in all three cell lines .

Article Snippet: Human prostate cancer LNCaP, PC3 and LAPC3 cells (American Type Culture Collection, Manassas, VA) were cultured and maintained at 37°C in a 5% CO2 atmosphere in RPMI-1640 medium supplemented with 5% fetal bovine serum, 100 units/mL penicillin and 100 μg/mL streptomycin.

Techniques: Cell Culture, TUNEL Assay, Staining, Fluorescence, Microscopy

Journal: American Journal of Cancer Research

Article Title: Immuno-PET imaging of VEGFR-2 expression in prostate cancer with 89 Zr-labeled ramucirumab

doi:

Figure Lengend Snippet: The VEGFR-2 expression in prostate cancer (PrCa) cell lines measured by flow cytometry. The percentage of cell counts are from the same gate of top positive cells in fluorescent intensity in the three PrCa cell lines. Sample groups: Anti-r 2nd Ab only, the controls engaging with Alexa488 anti-rabbit secondary antibody only; Anti-h 2nd Ab only, the controls engaging with Alexa488 anti-human secondary antibody only; 55B11 as 1st Ab, the samples engaging with rabbit anti-human 55B11 as the primary antibody; Df-R as 1st Ab, the samples engaging with deferoxamine-conjugated Ramucirumab as the primary antibody (for PC-3 cell line, n = 4; for LAPC-4 and LNCAP cell lines, n = 3).

Article Snippet: X.0.7; Tree Star, Inc.; Ashland, OR), shift values of the top 1% of the positive counts in fluorescent intensity were gated on the histograms of LAPC-4 blank cells as the baseline control.

Techniques: Expressing, Flow Cytometry

Journal: American Journal of Cancer Research

Article Title: Immuno-PET imaging of VEGFR-2 expression in prostate cancer with 89 Zr-labeled ramucirumab

doi:

Figure Lengend Snippet: The uptake kinetics of the region of interest (ROI) in the positron emission tomography (PET) images of the three prostate cancer (PrCa) models (for the models bearing PC-3 tumors without/with pre-blockade, n = 3; for the models bearing LAPC-4 and LNCAP tumors, n = 4). P < 0.05.

Article Snippet: X.0.7; Tree Star, Inc.; Ashland, OR), shift values of the top 1% of the positive counts in fluorescent intensity were gated on the histograms of LAPC-4 blank cells as the baseline control.

Techniques: Positron Emission Tomography

Journal: American Journal of Cancer Research

Article Title: Immuno-PET imaging of VEGFR-2 expression in prostate cancer with 89 Zr-labeled ramucirumab

doi:

Figure Lengend Snippet: The typical positron emission tomography (PET) maximum-intensity projections (MIP) of the PC-3 (unblocked/pre-blocked), LNCAP and LAPC-4 prostate cancer (PrCa) models. Arrows point to the xenograft tumor of each group at 96 h p.i.

Article Snippet: X.0.7; Tree Star, Inc.; Ashland, OR), shift values of the top 1% of the positive counts in fluorescent intensity were gated on the histograms of LAPC-4 blank cells as the baseline control.

Techniques: Positron Emission Tomography

Journal: American Journal of Cancer Research

Article Title: Immuno-PET imaging of VEGFR-2 expression in prostate cancer with 89 Zr-labeled ramucirumab

doi:

Figure Lengend Snippet: The radioactive ex-vivo bio-distribution in the tumors and major organs of the three prostate cancer (PrCa) models (for the models bearing PC-3 tumors without/with blockade, n = 3; for the models bearing LAPC-4 and LNCAP tumors, n = 4). P < 0.05.

Article Snippet: X.0.7; Tree Star, Inc.; Ashland, OR), shift values of the top 1% of the positive counts in fluorescent intensity were gated on the histograms of LAPC-4 blank cells as the baseline control.

Techniques: Ex Vivo