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  • lapc4  (ATCC)
    94
    ATCC lapc4
    Lapc4, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lapc4/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lapc4 - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

    lapc 4  (ATCC)
    99
    ATCC lapc 4
    FOXO1 interacts directly with ERG. A, Western blot analysis of endogenous FOXO1 and ERG proteins in VCaP cells immunoprecipitated by indicated antibody or non-specific IgG. B, Western blot analysis of ectopically expressed proteins immunoprecipitated by indicated antibody or non-specific IgG in PC-3 cells transfected with indicated Flag- and Myc-tagged plasmids. C , Schematic depicting a set of GST-ERG recombinant protein constructs. D, Coomassie Blue staining of GST and GST-ERG recombinant proteins purified from bacteria (bottom), and Western blot analysis of FOXO1 proteins in <t>LAPC-4</t> cells pulled down by GST recombinant proteins (top). Red arrow, non-specific band. * in red, unexpected, but reproducible band shift. * in black, denotes expected fragment size. E, Schematic depicting a set of GST-FOXO1 recombinant protein constructs. F, GelCode Blue staining of GST and GST-FOXO1 recombinant proteins purified from bacteria (bottom), and Western blot analysis of in vitro transcribed and translated ERG proteins pulled down by GST recombinant proteins (top).
    Lapc 4, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lapc 4/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lapc 4 - by Bioz Stars, 2022-08
    99/100 stars
      Buy from Supplier

    99
    ATCC lapc 4 human prostate cancer cell lines
    Arctigenin inhibits xenograft prostate tumor growth in high-fat diet fed SCID mice. Male SCID mice were fed either a high-fat (HF) diet or a regular low-fat (LF) diet (n = 10 per group). One week later, mice were inoculated subcutaneously with 5 × 10 5 androgen-sensitive human prostate cancer <t>LAPC-4</t> cells. The intervention started one week post tumor cell inoculation when tumors reached a volume of around 10 mm 3 . Mice were treated with either arctigenin at 50 mg/kg or vehicle control. Tumor size was measured once a week using calipers and tumor volume ( A ) calculated using the formula: length × width × height × 0.5236. Tumor weight ( B ) was measured at mouse sacrifice. Arctigenin was detected in blood and tumor from arctigenin supplemented mice ( C ) with HPLC coupled with CoulArray electrochemical detector after extraction with ethyl acetate. Data are presented as mean ± SD. Con: vehicle control; Arc: arctigenin. *Compared to control, P
    Lapc 4 Human Prostate Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lapc 4 human prostate cancer cell lines/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lapc 4 human prostate cancer cell lines - by Bioz Stars, 2022-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    FOXO1 interacts directly with ERG. A, Western blot analysis of endogenous FOXO1 and ERG proteins in VCaP cells immunoprecipitated by indicated antibody or non-specific IgG. B, Western blot analysis of ectopically expressed proteins immunoprecipitated by indicated antibody or non-specific IgG in PC-3 cells transfected with indicated Flag- and Myc-tagged plasmids. C , Schematic depicting a set of GST-ERG recombinant protein constructs. D, Coomassie Blue staining of GST and GST-ERG recombinant proteins purified from bacteria (bottom), and Western blot analysis of FOXO1 proteins in LAPC-4 cells pulled down by GST recombinant proteins (top). Red arrow, non-specific band. * in red, unexpected, but reproducible band shift. * in black, denotes expected fragment size. E, Schematic depicting a set of GST-FOXO1 recombinant protein constructs. F, GelCode Blue staining of GST and GST-FOXO1 recombinant proteins purified from bacteria (bottom), and Western blot analysis of in vitro transcribed and translated ERG proteins pulled down by GST recombinant proteins (top).

    Journal: Cancer research

    Article Title: Loss of FOXO1 cooperates with TMPRSS2-ERG overexpression to promote prostate tumorigenesis and cell invasion

    doi: 10.1158/0008-5472.CAN-17-0686

    Figure Lengend Snippet: FOXO1 interacts directly with ERG. A, Western blot analysis of endogenous FOXO1 and ERG proteins in VCaP cells immunoprecipitated by indicated antibody or non-specific IgG. B, Western blot analysis of ectopically expressed proteins immunoprecipitated by indicated antibody or non-specific IgG in PC-3 cells transfected with indicated Flag- and Myc-tagged plasmids. C , Schematic depicting a set of GST-ERG recombinant protein constructs. D, Coomassie Blue staining of GST and GST-ERG recombinant proteins purified from bacteria (bottom), and Western blot analysis of FOXO1 proteins in LAPC-4 cells pulled down by GST recombinant proteins (top). Red arrow, non-specific band. * in red, unexpected, but reproducible band shift. * in black, denotes expected fragment size. E, Schematic depicting a set of GST-FOXO1 recombinant protein constructs. F, GelCode Blue staining of GST and GST-FOXO1 recombinant proteins purified from bacteria (bottom), and Western blot analysis of in vitro transcribed and translated ERG proteins pulled down by GST recombinant proteins (top).

    Article Snippet: The cell lines VCaP, PC-3, and LAPC-4 were purchased from ATCC (Manassas, VA) and authenticated via STR profiling.

    Techniques: Western Blot, Immunoprecipitation, Transfection, Recombinant, Construct, Staining, Purification, Electrophoretic Mobility Shift Assay, In Vitro

    Arctigenin inhibits xenograft prostate tumor growth in high-fat diet fed SCID mice. Male SCID mice were fed either a high-fat (HF) diet or a regular low-fat (LF) diet (n = 10 per group). One week later, mice were inoculated subcutaneously with 5 × 10 5 androgen-sensitive human prostate cancer LAPC-4 cells. The intervention started one week post tumor cell inoculation when tumors reached a volume of around 10 mm 3 . Mice were treated with either arctigenin at 50 mg/kg or vehicle control. Tumor size was measured once a week using calipers and tumor volume ( A ) calculated using the formula: length × width × height × 0.5236. Tumor weight ( B ) was measured at mouse sacrifice. Arctigenin was detected in blood and tumor from arctigenin supplemented mice ( C ) with HPLC coupled with CoulArray electrochemical detector after extraction with ethyl acetate. Data are presented as mean ± SD. Con: vehicle control; Arc: arctigenin. *Compared to control, P

    Journal: Scientific Reports

    Article Title: Arctigenin inhibits prostate tumor growth in high-fat diet fed mice through dual actions on adipose tissue and tumor

    doi: 10.1038/s41598-020-58354-3

    Figure Lengend Snippet: Arctigenin inhibits xenograft prostate tumor growth in high-fat diet fed SCID mice. Male SCID mice were fed either a high-fat (HF) diet or a regular low-fat (LF) diet (n = 10 per group). One week later, mice were inoculated subcutaneously with 5 × 10 5 androgen-sensitive human prostate cancer LAPC-4 cells. The intervention started one week post tumor cell inoculation when tumors reached a volume of around 10 mm 3 . Mice were treated with either arctigenin at 50 mg/kg or vehicle control. Tumor size was measured once a week using calipers and tumor volume ( A ) calculated using the formula: length × width × height × 0.5236. Tumor weight ( B ) was measured at mouse sacrifice. Arctigenin was detected in blood and tumor from arctigenin supplemented mice ( C ) with HPLC coupled with CoulArray electrochemical detector after extraction with ethyl acetate. Data are presented as mean ± SD. Con: vehicle control; Arc: arctigenin. *Compared to control, P

    Article Snippet: Cell culture and adipocyte differentiation Androgen-sensitive LNCaP and LAPC-4 human prostate cancer cell lines were purchased from ATCC (Chicago, IL, USA), maintained in RPMI 1640 medium supplemented with 10% (v:v) of fetal bovine serum (FBS), 100 IU/mL of penicillin and 100 µg/mL of streptomycin at 37 °C in a 5% CO2 incubator.

    Techniques: Mouse Assay, High Performance Liquid Chromatography