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  • lapc4  (ATCC)
    96
    ATCC lapc4
    Chronic-ADT in hypoxia induces resistance to targeted disruption of androgen/AR-axis. a Schematic representation of repeated-ADT treatment of LNCaP and <t>LAPC4</t> cells in hypoxia (1% O 2 ) or normoxia (20% O 2 ). Cells were cultured in charcoal-stripped serum (CSS) media with or without 1 nM synthetic androgen R1881 (R1881 or ADT). Each round/cycle of treatment lasted 48 h, and the survived cells were recovered and re-plated for the same treatment in the following cycle. b Dose-dependent sensitivity of LNCaP clones to enzalutamide. Cells derived from a were cultured with CSS + R1881, and treated by increasing doses of enzalutamide in normoxia or hypoxia for 96 h. Afterwards, cell growth and viability were determined based on viable cells quantitated with SYTO 60 fluorescence staining and imaging. The AdtHs is represented by purple line. All values were mean ± s.d. relative to solvent-treated controls, n = 3. * P
    Lapc4, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1344 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher lapcs
    Chronic-ADT in hypoxia induces resistance to targeted disruption of androgen/AR-axis. a Schematic representation of repeated-ADT treatment of LNCaP and <t>LAPC4</t> cells in hypoxia (1% O 2 ) or normoxia (20% O 2 ). Cells were cultured in charcoal-stripped serum (CSS) media with or without 1 nM synthetic androgen R1881 (R1881 or ADT). Each round/cycle of treatment lasted 48 h, and the survived cells were recovered and re-plated for the same treatment in the following cycle. b Dose-dependent sensitivity of LNCaP clones to enzalutamide. Cells derived from a were cultured with CSS + R1881, and treated by increasing doses of enzalutamide in normoxia or hypoxia for 96 h. Afterwards, cell growth and viability were determined based on viable cells quantitated with SYTO 60 fluorescence staining and imaging. The AdtHs is represented by purple line. All values were mean ± s.d. relative to solvent-treated controls, n = 3. * P
    Lapcs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    BioLegend apc anti human cd24
    Chronic-ADT in hypoxia induces resistance to targeted disruption of androgen/AR-axis. a Schematic representation of repeated-ADT treatment of LNCaP and <t>LAPC4</t> cells in hypoxia (1% O 2 ) or normoxia (20% O 2 ). Cells were cultured in charcoal-stripped serum (CSS) media with or without 1 nM synthetic androgen R1881 (R1881 or ADT). Each round/cycle of treatment lasted 48 h, and the survived cells were recovered and re-plated for the same treatment in the following cycle. b Dose-dependent sensitivity of LNCaP clones to enzalutamide. Cells derived from a were cultured with CSS + R1881, and treated by increasing doses of enzalutamide in normoxia or hypoxia for 96 h. Afterwards, cell growth and viability were determined based on viable cells quantitated with SYTO 60 fluorescence staining and imaging. The AdtHs is represented by purple line. All values were mean ± s.d. relative to solvent-treated controls, n = 3. * P
    Apc Anti Human Cd24, supplied by BioLegend, used in various techniques. Bioz Stars score: 95/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Becton Dickinson lapc 4 cells
    Tumor concentration of unconjugated steroids (4-dione, T, DHT and ADT) in <t>LAPC-4</t> cells xenografted in nude mice and treated for 63 days. At the end of the xenograft experiment (see Fig 2 ), the tumors were collected and steroids measured by LC/MS-MS. CTX+4-dione group is significantly different (p
    Lapc 4 Cells, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    DDC Medical lapc 4 cells
    Functional Characterization of Total MEIS Knockdown <t>LAPC-4</t> Cells (A) Assessment of MEIS1 and MEIS2 knockdown in LAPC-4 cell using RT-PCR (top) and Western blot analysis (bottom). Two shRNA constructs against MEIS1 and two shRNA constructs against MEIS2 were utilized to control for off-target effects. To determine MEIS1 and MEIS2 protein levels after shRNA depletion, western blot analyses was conducted using a mouse monoclonal targeting both MEIS1 and MEIS2 (Pan-MEIS). (B) Xenograft growth of MEIS1 and MEIS2 double knockdown LAPC-4 cells injected subcutaneously on the flanks of hormonally-intact male nude mice compared to non-silencing controls. Differences in tumor volumes at days 25, 27 and 29 post-injection were statistically significant (Control N=8; double knockdown N=8; * p -value
    Lapc 4 Cells, supplied by DDC Medical, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Chronic-ADT in hypoxia induces resistance to targeted disruption of androgen/AR-axis. a Schematic representation of repeated-ADT treatment of LNCaP and LAPC4 cells in hypoxia (1% O 2 ) or normoxia (20% O 2 ). Cells were cultured in charcoal-stripped serum (CSS) media with or without 1 nM synthetic androgen R1881 (R1881 or ADT). Each round/cycle of treatment lasted 48 h, and the survived cells were recovered and re-plated for the same treatment in the following cycle. b Dose-dependent sensitivity of LNCaP clones to enzalutamide. Cells derived from a were cultured with CSS + R1881, and treated by increasing doses of enzalutamide in normoxia or hypoxia for 96 h. Afterwards, cell growth and viability were determined based on viable cells quantitated with SYTO 60 fluorescence staining and imaging. The AdtHs is represented by purple line. All values were mean ± s.d. relative to solvent-treated controls, n = 3. * P

    Journal: Nature Communications

    Article Title: Interplay between hypoxia and androgen controls a metabolic switch conferring resistance to androgen/AR-targeted therapy

    doi: 10.1038/s41467-018-07411-7

    Figure Lengend Snippet: Chronic-ADT in hypoxia induces resistance to targeted disruption of androgen/AR-axis. a Schematic representation of repeated-ADT treatment of LNCaP and LAPC4 cells in hypoxia (1% O 2 ) or normoxia (20% O 2 ). Cells were cultured in charcoal-stripped serum (CSS) media with or without 1 nM synthetic androgen R1881 (R1881 or ADT). Each round/cycle of treatment lasted 48 h, and the survived cells were recovered and re-plated for the same treatment in the following cycle. b Dose-dependent sensitivity of LNCaP clones to enzalutamide. Cells derived from a were cultured with CSS + R1881, and treated by increasing doses of enzalutamide in normoxia or hypoxia for 96 h. Afterwards, cell growth and viability were determined based on viable cells quantitated with SYTO 60 fluorescence staining and imaging. The AdtHs is represented by purple line. All values were mean ± s.d. relative to solvent-treated controls, n = 3. * P

    Article Snippet: Androgen-dependent prostate cancer cell lines LNCaP and LAPC4 were purchased from ATCC.

    Techniques: Cell Culture, Clone Assay, Derivative Assay, Fluorescence, Staining, Imaging

    FOXO1 interacts directly with ERG. A, Western blot analysis of endogenous FOXO1 and ERG proteins in VCaP cells immunoprecipitated by indicated antibody or non-specific IgG. B, Western blot analysis of ectopically expressed proteins immunoprecipitated by indicated antibody or non-specific IgG in PC-3 cells transfected with indicated Flag- and Myc-tagged plasmids. C , Schematic depicting a set of GST-ERG recombinant protein constructs. D, Coomassie Blue staining of GST and GST-ERG recombinant proteins purified from bacteria (bottom), and Western blot analysis of FOXO1 proteins in LAPC-4 cells pulled down by GST recombinant proteins (top). Red arrow, non-specific band. * in red, unexpected, but reproducible band shift. * in black, denotes expected fragment size. E, Schematic depicting a set of GST-FOXO1 recombinant protein constructs. F, GelCode Blue staining of GST and GST-FOXO1 recombinant proteins purified from bacteria (bottom), and Western blot analysis of in vitro transcribed and translated ERG proteins pulled down by GST recombinant proteins (top).

    Journal: Cancer research

    Article Title: Loss of FOXO1 cooperates with TMPRSS2-ERG overexpression to promote prostate tumorigenesis and cell invasion

    doi: 10.1158/0008-5472.CAN-17-0686

    Figure Lengend Snippet: FOXO1 interacts directly with ERG. A, Western blot analysis of endogenous FOXO1 and ERG proteins in VCaP cells immunoprecipitated by indicated antibody or non-specific IgG. B, Western blot analysis of ectopically expressed proteins immunoprecipitated by indicated antibody or non-specific IgG in PC-3 cells transfected with indicated Flag- and Myc-tagged plasmids. C , Schematic depicting a set of GST-ERG recombinant protein constructs. D, Coomassie Blue staining of GST and GST-ERG recombinant proteins purified from bacteria (bottom), and Western blot analysis of FOXO1 proteins in LAPC-4 cells pulled down by GST recombinant proteins (top). Red arrow, non-specific band. * in red, unexpected, but reproducible band shift. * in black, denotes expected fragment size. E, Schematic depicting a set of GST-FOXO1 recombinant protein constructs. F, GelCode Blue staining of GST and GST-FOXO1 recombinant proteins purified from bacteria (bottom), and Western blot analysis of in vitro transcribed and translated ERG proteins pulled down by GST recombinant proteins (top).

    Article Snippet: The cell lines VCaP, PC-3, and LAPC-4 were purchased from ATCC (Manassas, VA) and authenticated via STR profiling.

    Techniques: Western Blot, Immunoprecipitation, Transfection, Recombinant, Construct, Staining, Purification, Electrophoretic Mobility Shift Assay, In Vitro

    Inhibition of xenograft tumor growth, blood and tissue levels of arctigenin, and reduced expression of growth factors in SCID mice. Male SCID mice (n=10 per group) were inoculated subcutaneously with 5×10 5 androgen-sensitive LAPC-4 prostate tumor cells. The intervention treatments started either one week after (A, B) or two weeks prior to (C, D) the tumor cell inoculation. Arctigenin was administered daily through oral gavage at dose of 50mg/kg or 100mg/kg bw. Tumor size was measured twice a week using calipers and tumor volume calculated using the formula: length × width × height × 0.5236. Tumor weight was measured at mouse sacrifice. Blood and tumor levels of arctigenin (E) was detected with HPLC coupled with CoulArray electrochemical detector after extraction with ethyl acetate. The concentrations of 8 growth factors in tumor tissues were simultaneously measured using a Human Growth Factor ELISA Strip II kit (F). Data are presented as mean ± SD. Arc: arctigenin. Compared to control, *P

    Journal: Clinical nutrition experimental

    Article Title: Arctigenin inhibits prostate tumor cell growth in vitro and in vivo

    doi: 10.1016/j.yclnex.2017.04.001

    Figure Lengend Snippet: Inhibition of xenograft tumor growth, blood and tissue levels of arctigenin, and reduced expression of growth factors in SCID mice. Male SCID mice (n=10 per group) were inoculated subcutaneously with 5×10 5 androgen-sensitive LAPC-4 prostate tumor cells. The intervention treatments started either one week after (A, B) or two weeks prior to (C, D) the tumor cell inoculation. Arctigenin was administered daily through oral gavage at dose of 50mg/kg or 100mg/kg bw. Tumor size was measured twice a week using calipers and tumor volume calculated using the formula: length × width × height × 0.5236. Tumor weight was measured at mouse sacrifice. Blood and tumor levels of arctigenin (E) was detected with HPLC coupled with CoulArray electrochemical detector after extraction with ethyl acetate. The concentrations of 8 growth factors in tumor tissues were simultaneously measured using a Human Growth Factor ELISA Strip II kit (F). Data are presented as mean ± SD. Arc: arctigenin. Compared to control, *P

    Article Snippet: The androgen-sensitive LNCaP and LAPC-4 human prostate cancer cell lines were purchased from ATCC (Chicago, IL, USA).

    Techniques: Inhibition, Expressing, Mouse Assay, High Performance Liquid Chromatography, Enzyme-linked Immunosorbent Assay, Stripping Membranes

    TIMP-1 promotes in vivo growth of prostate cancers. A . Establishment of pooled populations of PC3, 22RV1, and LAPC-4 cells expressing v5 epitope tagged TIMP-1 or transduced with the empty expression vector alone (controls). Secreted v5-tagged TIMP-1 was detected by anti-v5 mAb (Invitrogen). B . TIMP-1 promotes PC3 and 22RV1 prostate cancer growth in vivo . Weights of the tumors derived from PC3 and 22RV1 prostate cancer cells expressing TIMP-1v5 or infected with the empty expression constructs (PC3-control and 22RV1-control) were measured 5 or 10-weeks, respectively, after subcutaneous implantation of the cancer cells into Rag-2/II2rg mice (Taconic). n=6. * p

    Journal: PLoS ONE

    Article Title: TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression

    doi: 10.1371/journal.pone.0077366

    Figure Lengend Snippet: TIMP-1 promotes in vivo growth of prostate cancers. A . Establishment of pooled populations of PC3, 22RV1, and LAPC-4 cells expressing v5 epitope tagged TIMP-1 or transduced with the empty expression vector alone (controls). Secreted v5-tagged TIMP-1 was detected by anti-v5 mAb (Invitrogen). B . TIMP-1 promotes PC3 and 22RV1 prostate cancer growth in vivo . Weights of the tumors derived from PC3 and 22RV1 prostate cancer cells expressing TIMP-1v5 or infected with the empty expression constructs (PC3-control and 22RV1-control) were measured 5 or 10-weeks, respectively, after subcutaneous implantation of the cancer cells into Rag-2/II2rg mice (Taconic). n=6. * p

    Article Snippet: Human prostate cancer LAPC-4 cells were obtained from the ATCC Patent Depository.

    Techniques: In Vivo, Expressing, Transduction, Plasmid Preparation, Derivative Assay, Infection, Construct, Mouse Assay

    TIMP-1 enhances expression of Snail, MMP-2, and MMP-9 in prostate cancers. Expression levels of several EMT markers, such as Snail (A, E, I, M), Slug (B, F, J, N), MMP-2 (C, G, K, O), and MMP-9 (D, H, L, P) were assessed on the tumor sections derived from PC3/LAPC-4 control cells (A-D and I-L, respectively) and PC3/LAPC-4-TIMP-1 cells (E-H and M-P, respectively). The results show that TIMP-1 enhances expression of Snail, MMP-2, and MMP-9 but not Slug in the prostate cancer tissues. Bar, 50 µm in A, B, E, F, I, J, M, N and 100 µm in C, D, G, H, K, L, O, P.

    Journal: PLoS ONE

    Article Title: TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression

    doi: 10.1371/journal.pone.0077366

    Figure Lengend Snippet: TIMP-1 enhances expression of Snail, MMP-2, and MMP-9 in prostate cancers. Expression levels of several EMT markers, such as Snail (A, E, I, M), Slug (B, F, J, N), MMP-2 (C, G, K, O), and MMP-9 (D, H, L, P) were assessed on the tumor sections derived from PC3/LAPC-4 control cells (A-D and I-L, respectively) and PC3/LAPC-4-TIMP-1 cells (E-H and M-P, respectively). The results show that TIMP-1 enhances expression of Snail, MMP-2, and MMP-9 but not Slug in the prostate cancer tissues. Bar, 50 µm in A, B, E, F, I, J, M, N and 100 µm in C, D, G, H, K, L, O, P.

    Article Snippet: Human prostate cancer LAPC-4 cells were obtained from the ATCC Patent Depository.

    Techniques: Expressing, Derivative Assay

    Developmental reprogramming of PCa Cells to a stem-like intermediate ( A ) Chronic AD of LNCaP cells upregulated genes associated with N/NC stem cells and derivative tissues. LNCaP cells (Left) cultured in androgen-depleted medium for 15-days undergo a morphological transformation (Middle), and overexpress genes associated with N/NC stem cells and derivative tissues shown on heat-mapping (Right). ( B ) Representative phase-contrast images of LNCaP cells over two weeks of culture in STM. ( C ) Representative phase-contrast images of VCaP, LAPC4 or 22Rv1 spheroids after 14-day STM-mediated reprogramming. Scale bars represent 100 μm. ( D ) Proliferation assays of parental LNCaP (P) and reprogrammed stem-like LNCaP cells (SL) showed that the doubling time of LNCaP-SL is significantly greater than parentals. Mean ± standard error; ** P

    Journal: Oncotarget

    Article Title: Therapy-induced developmental reprogramming of prostate cancer cells and acquired therapy resistance

    doi: 10.18632/oncotarget.14850

    Figure Lengend Snippet: Developmental reprogramming of PCa Cells to a stem-like intermediate ( A ) Chronic AD of LNCaP cells upregulated genes associated with N/NC stem cells and derivative tissues. LNCaP cells (Left) cultured in androgen-depleted medium for 15-days undergo a morphological transformation (Middle), and overexpress genes associated with N/NC stem cells and derivative tissues shown on heat-mapping (Right). ( B ) Representative phase-contrast images of LNCaP cells over two weeks of culture in STM. ( C ) Representative phase-contrast images of VCaP, LAPC4 or 22Rv1 spheroids after 14-day STM-mediated reprogramming. Scale bars represent 100 μm. ( D ) Proliferation assays of parental LNCaP (P) and reprogrammed stem-like LNCaP cells (SL) showed that the doubling time of LNCaP-SL is significantly greater than parentals. Mean ± standard error; ** P

    Article Snippet: Cell culture and xenografts LNCaP, VCaP, 22Rv1 (ATCC, Manassas, VA) and LAPC4 cell line (obtained from Dr. Charles Sawyer), were maintained in phenol red-free ATCC-recommended media.

    Techniques: Cell Culture, Transformation Assay

    Increased regulation of SGK1 by the GR following AR antagonism. A mRNA was collected from LAPC4 and 22Rv1 cells treated for 2 h under various hormonal conditions, and SGK1 gene expression was assessed by qRT-PCR. B Both 22Rv1 and LAPC4 cells were treated for 3 days in either vehicle, R1881 (1 nM), or R1881 + MDV3100 (10 μM)-containing media. Conditions specifying dexamethasone treatment were stimulated with Dex (100 nM) for 1 h prior to chromatin harvest. Targeted qPCR was performed for the region within the SGK1 promoter after ChIP, and y -values represent fold chromatin enrichment (relative to IgG controls for each condition). Error bars represent standard deviation of mean cycle threshold values, and asterisk indicates p

    Journal: Hormones & cancer

    Article Title: Glucocorticoid Receptor Activity Contributes to Resistance to Androgen-Targeted Therapy in Prostate Cancer

    doi: 10.1007/s12672-014-0173-2

    Figure Lengend Snippet: Increased regulation of SGK1 by the GR following AR antagonism. A mRNA was collected from LAPC4 and 22Rv1 cells treated for 2 h under various hormonal conditions, and SGK1 gene expression was assessed by qRT-PCR. B Both 22Rv1 and LAPC4 cells were treated for 3 days in either vehicle, R1881 (1 nM), or R1881 + MDV3100 (10 μM)-containing media. Conditions specifying dexamethasone treatment were stimulated with Dex (100 nM) for 1 h prior to chromatin harvest. Targeted qPCR was performed for the region within the SGK1 promoter after ChIP, and y -values represent fold chromatin enrichment (relative to IgG controls for each condition). Error bars represent standard deviation of mean cycle threshold values, and asterisk indicates p

    Article Snippet: The LAPC4 human PC cell line was cultured in IMDM (ATCC) supplemented with 10 % FCS, 1 % penicillin/streptomycin, and 1 nM R1881 (Sigma-Aldrich), and VCaP cells lines were cultured in DMEM (Mediatech) supplemented with 10 % FCS and 1 % penicillin/streptomycin.

    Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Chromatin Immunoprecipitation, Standard Deviation

    GR expression in prostate cancer following androgen signaling inhibition. A Western blot of GR and AR expression for PC cell lines PC3, DU145, LNCaP, LAPC4, CWR-22Rv1, and VCaP. B NRC31 ( GR ) mRNA expression ( above ) and protein expression ( below ) in PC cell lines after long-term ( > 30 days) treatment with AR antagonist MDV3100. Error bars represent standard error of the mean, and asterisk indicates p

    Journal: Hormones & cancer

    Article Title: Glucocorticoid Receptor Activity Contributes to Resistance to Androgen-Targeted Therapy in Prostate Cancer

    doi: 10.1007/s12672-014-0173-2

    Figure Lengend Snippet: GR expression in prostate cancer following androgen signaling inhibition. A Western blot of GR and AR expression for PC cell lines PC3, DU145, LNCaP, LAPC4, CWR-22Rv1, and VCaP. B NRC31 ( GR ) mRNA expression ( above ) and protein expression ( below ) in PC cell lines after long-term ( > 30 days) treatment with AR antagonist MDV3100. Error bars represent standard error of the mean, and asterisk indicates p

    Article Snippet: The LAPC4 human PC cell line was cultured in IMDM (ATCC) supplemented with 10 % FCS, 1 % penicillin/streptomycin, and 1 nM R1881 (Sigma-Aldrich), and VCaP cells lines were cultured in DMEM (Mediatech) supplemented with 10 % FCS and 1 % penicillin/streptomycin.

    Techniques: Expressing, Inhibition, Western Blot

    GR expression/activation levels following AR inhibitor therapy. Hormonal therapies utilized included: R1881 (1 nM), a synthetic androgen; dexamethasone (Dex, 100 nM), a GR agonist; and MDV3100 (MDV, 10 μM), an AR antagonist. A 22Rv1 and LAPC4 GR mRNA expression analysis by qRT-PCR after 3 days of treatment; normalized to GAPDH and depicted as fold change relative to vehicle. B Western blot analysis of 22Rv1 and LAPC4 GR and AR protein expression after 3 and 7 days of treatment. All conditions were treated with Dex and R1881. Asterisk denotes AR splice variants within 22Rv1 cells. C 22Rv1 and LAPC4 cells were treated for 14 days under various conditions

    Journal: Hormones & cancer

    Article Title: Glucocorticoid Receptor Activity Contributes to Resistance to Androgen-Targeted Therapy in Prostate Cancer

    doi: 10.1007/s12672-014-0173-2

    Figure Lengend Snippet: GR expression/activation levels following AR inhibitor therapy. Hormonal therapies utilized included: R1881 (1 nM), a synthetic androgen; dexamethasone (Dex, 100 nM), a GR agonist; and MDV3100 (MDV, 10 μM), an AR antagonist. A 22Rv1 and LAPC4 GR mRNA expression analysis by qRT-PCR after 3 days of treatment; normalized to GAPDH and depicted as fold change relative to vehicle. B Western blot analysis of 22Rv1 and LAPC4 GR and AR protein expression after 3 and 7 days of treatment. All conditions were treated with Dex and R1881. Asterisk denotes AR splice variants within 22Rv1 cells. C 22Rv1 and LAPC4 cells were treated for 14 days under various conditions

    Article Snippet: The LAPC4 human PC cell line was cultured in IMDM (ATCC) supplemented with 10 % FCS, 1 % penicillin/streptomycin, and 1 nM R1881 (Sigma-Aldrich), and VCaP cells lines were cultured in DMEM (Mediatech) supplemented with 10 % FCS and 1 % penicillin/streptomycin.

    Techniques: Expressing, Activation Assay, Quantitative RT-PCR, Western Blot

    Increased regulation of PSA by the GR following AR antagonism. A mRNA was collected from LAPC4 and 22Rv1 cells treated for 2 h under various hormonal conditions, and PSA gene expression was assessed by qRT-PCR. B Both 22Rv1 and LAPC4 cells were treated for 3 days in either vehicle, R1881 (1 nM), or R1881 + MDV3100 (10 μM) containing media. Conditions specifying dexamethasone treatment were stimulated with Dex (100 nM) for 1 h prior to chromatin harvest. Targeted qPCR was performed for the PSA promoter after ChIP, and y -values represent fold chromatin enrichment (relative to IgG controls for each condition). Error bars represent standard deviation of mean cycle threshold values, and asterisk indicates p

    Journal: Hormones & cancer

    Article Title: Glucocorticoid Receptor Activity Contributes to Resistance to Androgen-Targeted Therapy in Prostate Cancer

    doi: 10.1007/s12672-014-0173-2

    Figure Lengend Snippet: Increased regulation of PSA by the GR following AR antagonism. A mRNA was collected from LAPC4 and 22Rv1 cells treated for 2 h under various hormonal conditions, and PSA gene expression was assessed by qRT-PCR. B Both 22Rv1 and LAPC4 cells were treated for 3 days in either vehicle, R1881 (1 nM), or R1881 + MDV3100 (10 μM) containing media. Conditions specifying dexamethasone treatment were stimulated with Dex (100 nM) for 1 h prior to chromatin harvest. Targeted qPCR was performed for the PSA promoter after ChIP, and y -values represent fold chromatin enrichment (relative to IgG controls for each condition). Error bars represent standard deviation of mean cycle threshold values, and asterisk indicates p

    Article Snippet: The LAPC4 human PC cell line was cultured in IMDM (ATCC) supplemented with 10 % FCS, 1 % penicillin/streptomycin, and 1 nM R1881 (Sigma-Aldrich), and VCaP cells lines were cultured in DMEM (Mediatech) supplemented with 10 % FCS and 1 % penicillin/streptomycin.

    Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Chromatin Immunoprecipitation, Standard Deviation

    Tumor concentration of unconjugated steroids (4-dione, T, DHT and ADT) in LAPC-4 cells xenografted in nude mice and treated for 63 days. At the end of the xenograft experiment (see Fig 2 ), the tumors were collected and steroids measured by LC/MS-MS. CTX+4-dione group is significantly different (p

    Journal: PLoS ONE

    Article Title: Investigation of the In Vitro and In Vivo efficiency of RM-532-105, a 17β-hydroxysteroid dehydrogenase type 3 inhibitor, in LAPC-4 prostate cancer cell and tumor models

    doi: 10.1371/journal.pone.0171871

    Figure Lengend Snippet: Tumor concentration of unconjugated steroids (4-dione, T, DHT and ADT) in LAPC-4 cells xenografted in nude mice and treated for 63 days. At the end of the xenograft experiment (see Fig 2 ), the tumors were collected and steroids measured by LC/MS-MS. CTX+4-dione group is significantly different (p

    Article Snippet: After receiving 4-dione for 3 days, 24 castrated mice as well as 6 non-castrated mice were inoculated by subcutaneous (s.c.) injection of 10 million LAPC-4 cells (in 0.1 mL of growth medium containing 30% Matrigel (BD Biosciences, Bedford, MA, USA)) into both mouse flanks via a 2.5-cm long 22-gauge needle.

    Techniques: Concentration Assay, Mouse Assay, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    Metabolism of 4-dione in LAPC-4 cells in the presence of RM-532-105 or Dutasteride. [ 14 C]-4-dione (50 nM) was incubated with 20,000 LAPC-4 cells in the absence or presence of RM-532-105 (0.1, 1, 5 and 10 μM) or Dutasteride (0.1 and 1 μM). After 3 days ( A ) or 5 days ( B ) of treatment, steroids were extracted from the culture medium, analyzed by TLC, and quantified.

    Journal: PLoS ONE

    Article Title: Investigation of the In Vitro and In Vivo efficiency of RM-532-105, a 17β-hydroxysteroid dehydrogenase type 3 inhibitor, in LAPC-4 prostate cancer cell and tumor models

    doi: 10.1371/journal.pone.0171871

    Figure Lengend Snippet: Metabolism of 4-dione in LAPC-4 cells in the presence of RM-532-105 or Dutasteride. [ 14 C]-4-dione (50 nM) was incubated with 20,000 LAPC-4 cells in the absence or presence of RM-532-105 (0.1, 1, 5 and 10 μM) or Dutasteride (0.1 and 1 μM). After 3 days ( A ) or 5 days ( B ) of treatment, steroids were extracted from the culture medium, analyzed by TLC, and quantified.

    Article Snippet: After receiving 4-dione for 3 days, 24 castrated mice as well as 6 non-castrated mice were inoculated by subcutaneous (s.c.) injection of 10 million LAPC-4 cells (in 0.1 mL of growth medium containing 30% Matrigel (BD Biosciences, Bedford, MA, USA)) into both mouse flanks via a 2.5-cm long 22-gauge needle.

    Techniques: Incubation, Thin Layer Chromatography

    Metabolism of 4-dione in LAPC-4 cells. [ 14 C]-4-dione (50 nM) was incubated with 20,000 LAPC-4 cells for 0, 3 and 5 days. Steroids were extracted from the culture medium, analyzed by TLC, and quantified.

    Journal: PLoS ONE

    Article Title: Investigation of the In Vitro and In Vivo efficiency of RM-532-105, a 17β-hydroxysteroid dehydrogenase type 3 inhibitor, in LAPC-4 prostate cancer cell and tumor models

    doi: 10.1371/journal.pone.0171871

    Figure Lengend Snippet: Metabolism of 4-dione in LAPC-4 cells. [ 14 C]-4-dione (50 nM) was incubated with 20,000 LAPC-4 cells for 0, 3 and 5 days. Steroids were extracted from the culture medium, analyzed by TLC, and quantified.

    Article Snippet: After receiving 4-dione for 3 days, 24 castrated mice as well as 6 non-castrated mice were inoculated by subcutaneous (s.c.) injection of 10 million LAPC-4 cells (in 0.1 mL of growth medium containing 30% Matrigel (BD Biosciences, Bedford, MA, USA)) into both mouse flanks via a 2.5-cm long 22-gauge needle.

    Techniques: Incubation, Thin Layer Chromatography

    Plasma concentration of unconjugated steroids (4-dione, T, DHT and ADT) in LAPC-4 cells xenografted in nude mice and treated for 63 days. At the end of the xenograft experiment (see Fig 2 ) and 3 h after the last injection, the blood was collected and steroids measured by LC/MS-MS. CTX+4-dione group is significantly different (p

    Journal: PLoS ONE

    Article Title: Investigation of the In Vitro and In Vivo efficiency of RM-532-105, a 17β-hydroxysteroid dehydrogenase type 3 inhibitor, in LAPC-4 prostate cancer cell and tumor models

    doi: 10.1371/journal.pone.0171871

    Figure Lengend Snippet: Plasma concentration of unconjugated steroids (4-dione, T, DHT and ADT) in LAPC-4 cells xenografted in nude mice and treated for 63 days. At the end of the xenograft experiment (see Fig 2 ) and 3 h after the last injection, the blood was collected and steroids measured by LC/MS-MS. CTX+4-dione group is significantly different (p

    Article Snippet: After receiving 4-dione for 3 days, 24 castrated mice as well as 6 non-castrated mice were inoculated by subcutaneous (s.c.) injection of 10 million LAPC-4 cells (in 0.1 mL of growth medium containing 30% Matrigel (BD Biosciences, Bedford, MA, USA)) into both mouse flanks via a 2.5-cm long 22-gauge needle.

    Techniques: Concentration Assay, Mouse Assay, Injection, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    Proliferation of androgen-sensitive LAPC-4 (AR + ) cells under different treatments. A) Effect of antiandrogen Casodex on proliferation induced by DHT. 3,000 cells were pre-incubated for 48 h, washed 4 times and treated 6 days with DHT, a natural potent androgen, and different concentrations (1.0, 2.0, 5.0 and 10 μM of Casodex). Significantly different from the control (CTL) p

    Journal: PLoS ONE

    Article Title: Investigation of the In Vitro and In Vivo efficiency of RM-532-105, a 17β-hydroxysteroid dehydrogenase type 3 inhibitor, in LAPC-4 prostate cancer cell and tumor models

    doi: 10.1371/journal.pone.0171871

    Figure Lengend Snippet: Proliferation of androgen-sensitive LAPC-4 (AR + ) cells under different treatments. A) Effect of antiandrogen Casodex on proliferation induced by DHT. 3,000 cells were pre-incubated for 48 h, washed 4 times and treated 6 days with DHT, a natural potent androgen, and different concentrations (1.0, 2.0, 5.0 and 10 μM of Casodex). Significantly different from the control (CTL) p

    Article Snippet: After receiving 4-dione for 3 days, 24 castrated mice as well as 6 non-castrated mice were inoculated by subcutaneous (s.c.) injection of 10 million LAPC-4 cells (in 0.1 mL of growth medium containing 30% Matrigel (BD Biosciences, Bedford, MA, USA)) into both mouse flanks via a 2.5-cm long 22-gauge needle.

    Techniques: Incubation, CTL Assay

    Tumor growth of human LAPC-4 cells xenografted in nude mice. Sham and castrated (CTX) mice received only the vehicle daily, whereas all the other castrated mice received daily s.c. 4-dione (200 μg), TP (200 μg) or 4-dione (200 μg) + RM-532-105 (50 mg/kg body weight) for 63 days. Data represent the mean ± SEM.

    Journal: PLoS ONE

    Article Title: Investigation of the In Vitro and In Vivo efficiency of RM-532-105, a 17β-hydroxysteroid dehydrogenase type 3 inhibitor, in LAPC-4 prostate cancer cell and tumor models

    doi: 10.1371/journal.pone.0171871

    Figure Lengend Snippet: Tumor growth of human LAPC-4 cells xenografted in nude mice. Sham and castrated (CTX) mice received only the vehicle daily, whereas all the other castrated mice received daily s.c. 4-dione (200 μg), TP (200 μg) or 4-dione (200 μg) + RM-532-105 (50 mg/kg body weight) for 63 days. Data represent the mean ± SEM.

    Article Snippet: After receiving 4-dione for 3 days, 24 castrated mice as well as 6 non-castrated mice were inoculated by subcutaneous (s.c.) injection of 10 million LAPC-4 cells (in 0.1 mL of growth medium containing 30% Matrigel (BD Biosciences, Bedford, MA, USA)) into both mouse flanks via a 2.5-cm long 22-gauge needle.

    Techniques: Mouse Assay

    RM-532-105 does not modulate the transcriptional activity of AR in vitro . LAPC-4 cells were infected with the PSEBC-TSTA-fl adenovirus (MOI = 5) and treated with testosterone (T) (100 or 1,000 pg/mL). The reporter system is able to quantitatively assess AR activity in LAPC-4 cells, even with castrated (CTX) levels of T (100 pg/mL). The addition of RM-532-105 compound to the CTX level of T (100 pg/mL) does not significantly contribute (p

    Journal: PLoS ONE

    Article Title: Investigation of the In Vitro and In Vivo efficiency of RM-532-105, a 17β-hydroxysteroid dehydrogenase type 3 inhibitor, in LAPC-4 prostate cancer cell and tumor models

    doi: 10.1371/journal.pone.0171871

    Figure Lengend Snippet: RM-532-105 does not modulate the transcriptional activity of AR in vitro . LAPC-4 cells were infected with the PSEBC-TSTA-fl adenovirus (MOI = 5) and treated with testosterone (T) (100 or 1,000 pg/mL). The reporter system is able to quantitatively assess AR activity in LAPC-4 cells, even with castrated (CTX) levels of T (100 pg/mL). The addition of RM-532-105 compound to the CTX level of T (100 pg/mL) does not significantly contribute (p

    Article Snippet: After receiving 4-dione for 3 days, 24 castrated mice as well as 6 non-castrated mice were inoculated by subcutaneous (s.c.) injection of 10 million LAPC-4 cells (in 0.1 mL of growth medium containing 30% Matrigel (BD Biosciences, Bedford, MA, USA)) into both mouse flanks via a 2.5-cm long 22-gauge needle.

    Techniques: Activity Assay, In Vitro, Infection

    Functional Characterization of Total MEIS Knockdown LAPC-4 Cells (A) Assessment of MEIS1 and MEIS2 knockdown in LAPC-4 cell using RT-PCR (top) and Western blot analysis (bottom). Two shRNA constructs against MEIS1 and two shRNA constructs against MEIS2 were utilized to control for off-target effects. To determine MEIS1 and MEIS2 protein levels after shRNA depletion, western blot analyses was conducted using a mouse monoclonal targeting both MEIS1 and MEIS2 (Pan-MEIS). (B) Xenograft growth of MEIS1 and MEIS2 double knockdown LAPC-4 cells injected subcutaneously on the flanks of hormonally-intact male nude mice compared to non-silencing controls. Differences in tumor volumes at days 25, 27 and 29 post-injection were statistically significant (Control N=8; double knockdown N=8; * p -value

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: MEIS1 and MEIS2 Expression and Prostate Cancer Progression: A Role For HOXB13 Binding Partners in Metastatic Disease

    doi: 10.1158/1078-0432.CCR-17-3673

    Figure Lengend Snippet: Functional Characterization of Total MEIS Knockdown LAPC-4 Cells (A) Assessment of MEIS1 and MEIS2 knockdown in LAPC-4 cell using RT-PCR (top) and Western blot analysis (bottom). Two shRNA constructs against MEIS1 and two shRNA constructs against MEIS2 were utilized to control for off-target effects. To determine MEIS1 and MEIS2 protein levels after shRNA depletion, western blot analyses was conducted using a mouse monoclonal targeting both MEIS1 and MEIS2 (Pan-MEIS). (B) Xenograft growth of MEIS1 and MEIS2 double knockdown LAPC-4 cells injected subcutaneously on the flanks of hormonally-intact male nude mice compared to non-silencing controls. Differences in tumor volumes at days 25, 27 and 29 post-injection were statistically significant (Control N=8; double knockdown N=8; * p -value

    Article Snippet: Cell authentication of LAPC-4 cells was confirmed via DDC Medical services (Fairfield, OH).

    Techniques: Functional Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot, shRNA, Construct, Injection, Mouse Assay