L-1340-5 Search Results


maclura pomifera agglutinin mpa lectin  (Vector Laboratories)
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Vector Laboratories maclura pomifera agglutinin mpa lectin
Demonstrating the presence of specific proteins in protein elutions indicating the presence in pellicle on each particle surface from each type of saliva secretion. How proteins detected is described: CBB – Coomassie staining, PAS – PAS staining, WB – Western blotting and <t> MPA </t> – <t> lectin </t> binding using Western blotting. Symbols indicate presence (+) or absence (−) in the three elutions: SDS1, SDS2, boil elution (WMS only, not all proteins tested). * indicates that detection levels were too low to determine protein binding pattern (N/A indicates that the protein is not present in the saliva).
Maclura Pomifera Agglutinin Mpa Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/L-1340-5/Unconjugated+Maclura+Pomifera+Lectin+(MPL)/pmc04097378-100-22-27
Average 92 stars, based on 1 article reviews
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Demonstrating the presence of specific proteins in protein elutions indicating the presence in pellicle on each particle surface from each type of saliva secretion. How proteins detected is described: CBB – Coomassie staining, PAS – PAS staining, WB – Western blotting and MPA – lectin binding using Western blotting. Symbols indicate presence (+) or absence (−) in the three elutions: SDS1, SDS2, boil elution (WMS only, not all proteins tested). * indicates that detection levels were too low to determine protein binding pattern (N/A indicates that the protein is not present in the saliva).

Journal: Colloids and Surfaces. B, Biointerfaces

Article Title: What interactions drive the salivary mucosal pellicle formation?

doi: 10.1016/j.colsurfb.2014.05.020

Figure Lengend Snippet: Demonstrating the presence of specific proteins in protein elutions indicating the presence in pellicle on each particle surface from each type of saliva secretion. How proteins detected is described: CBB – Coomassie staining, PAS – PAS staining, WB – Western blotting and MPA – lectin binding using Western blotting. Symbols indicate presence (+) or absence (−) in the three elutions: SDS1, SDS2, boil elution (WMS only, not all proteins tested). * indicates that detection levels were too low to determine protein binding pattern (N/A indicates that the protein is not present in the saliva).

Article Snippet: The membrane was then left to expose onto photographic film, developed and then fixed in the dark, followed by a water wash. Maclura pomifera agglutinin (MPA) lectin (Vector Laboratories Ltd., Peterborough, UK) was also used to visualise proteins containing a Galβ,1-3GalNAc group.

Techniques: Staining, Western Blot, Binding Assay, Protein Binding