Journal: Respiratory Research

Article Title: Systemic IL-26 correlates with improved asthma control in children sensitized to dog allergen

doi: 10.1186/s12931-024-02773-7

Figure Lengend Snippet: Serum cytokine concentrations in allergen-sensitized subjects vs. controls. IL-26 ( A ) and IL-17 A ( B ) serum concentrations were measured by ELISA in both non-allergen-sensitized and allergen-sensitized study subjects ( n = 17 and n = 60, respectively). Statistical significance was assessed using the Mann-Whitney test. Error bars represent mean ± SEM. Dashed horizonal line represents the lower limit of detection of the ELISA assay

Article Snippet: IL-26 was measured from serum using the Human IL-26 ELISA kit from Invitrogen (Carlsbad, CA, USA), which has an assay range between 3.3 and 800 pg/mL.

Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Respiratory Research

Article Title: Systemic IL-26 correlates with improved asthma control in children sensitized to dog allergen

doi: 10.1186/s12931-024-02773-7

Figure Lengend Snippet: Serum cytokine concentrations in subjects with and without asthma. Serum concentrations of IL-26 ( A ) and IL-17 A ( B ) were measured by ELISA in both non-allergen-sensitized and allergen-sensitized study subjects ( n = 17 and n = 60, respectively). Comparisons were made in subjects with or without asthma. Statistical significance was assessed using the Kruskal-Wallis test for multiple comparisons. Error bars represent mean ± SEM. Dashed horizonal line represents the lower limit of detection of the ELISA assay

Article Snippet: IL-26 was measured from serum using the Human IL-26 ELISA kit from Invitrogen (Carlsbad, CA, USA), which has an assay range between 3.3 and 800 pg/mL.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Respiratory Research

Article Title: Systemic IL-26 correlates with improved asthma control in children sensitized to dog allergen

doi: 10.1186/s12931-024-02773-7

Figure Lengend Snippet: Serum cytokine concentrations in subjects with and without eczema. Serum concentrations of IL-26 ( A ) and IL-17 A ( B ) were measured by ELISA in both non-allergen-sensitized and allergen-sensitized study subjects ( n = 17 and n = 60, respectively). Comparisons were made in subjects with or without eczema. Statistical significance was assessed using the Kruskal-Wallis test for multiple comparisons. Error bars represent mean ± SEM. Dashed horizonal line represents the lower limit of detection of the ELISA assay

Article Snippet: IL-26 was measured from serum using the Human IL-26 ELISA kit from Invitrogen (Carlsbad, CA, USA), which has an assay range between 3.3 and 800 pg/mL.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Respiratory Research

Article Title: Systemic IL-26 correlates with improved asthma control in children sensitized to dog allergen

doi: 10.1186/s12931-024-02773-7

Figure Lengend Snippet: Serum cytokine concentrations in subjects with and without allergic rhinitis. Serum concentrations of IL-26 ( A ) and IL-17 A ( B ) were measured by ELISA in both non-allergen-sensitized and allergen-sensitized study subjects ( n = 17 and n = 60, respectively). Comparisons were made in subjects with or without allergic rhinitis. Statistical significance was assessed using the Kruskal-Wallis test for multiple comparisons. Error bars represent mean ± SEM. Dashed horizonal line represents the lower limit of detection of the ELISA assay

Article Snippet: IL-26 was measured from serum using the Human IL-26 ELISA kit from Invitrogen (Carlsbad, CA, USA), which has an assay range between 3.3 and 800 pg/mL.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Respiratory Research

Article Title: Systemic IL-26 correlates with improved asthma control in children sensitized to dog allergen

doi: 10.1186/s12931-024-02773-7

Figure Lengend Snippet: Serum cytokine concentrations in subjects with and without food allergies. Serum concentrations of IL-26 ( A ) and IL-17 A ( B ) were measured by ELISA in both non-allergen-sensitized and allergen-sensitized study subjects ( n = 17 and n = 60, respectively). Comparisons were made in subjects with or without a self-reported history of one or more food allergies. Statistical significance was assessed using the Kruskal-Wallis test for multiple comparisons. Error bars represent mean ± SEM. Dashed horizonal line represents the lower limit of detection of the ELISA assay

Article Snippet: IL-26 was measured from serum using the Human IL-26 ELISA kit from Invitrogen (Carlsbad, CA, USA), which has an assay range between 3.3 and 800 pg/mL.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Respiratory Research

Article Title: Systemic IL-26 correlates with improved asthma control in children sensitized to dog allergen

doi: 10.1186/s12931-024-02773-7

Figure Lengend Snippet: Serum IL-26 concentrations correlate with serum IL-17 A in allergen-sensitized subjects. Serum concentrations of IL-26 and IL-17 A were measured by ELISA in allergen-sensitized subjects. Concentrations of each cytokine were plotted relative to each other for ( A ) all allergen-sensitized subjects ( n = 60) or ( B ) limited to only allergen-sensitized subjects with asthma ( n = 51). Statistical significance was assessed using the Spearman correlation coefficient

Article Snippet: IL-26 was measured from serum using the Human IL-26 ELISA kit from Invitrogen (Carlsbad, CA, USA), which has an assay range between 3.3 and 800 pg/mL.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Respiratory Research

Article Title: Systemic IL-26 correlates with improved asthma control in children sensitized to dog allergen

doi: 10.1186/s12931-024-02773-7

Figure Lengend Snippet: Serum IL-26 concentrations correlate with Asthma Control Test (ACT) Score in allergen-sensitized subjects. Serum concentrations of IL-26 were measured by ELISA in allergen-sensitized subjects with asthma ( n = 51) and plotted relative to ACT scores. Statistical significance was assessed using the Spearman correlation coefficient

Article Snippet: IL-26 was measured from serum using the Human IL-26 ELISA kit from Invitrogen (Carlsbad, CA, USA), which has an assay range between 3.3 and 800 pg/mL.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Respiratory Research

Article Title: Systemic IL-26 correlates with improved asthma control in children sensitized to dog allergen

doi: 10.1186/s12931-024-02773-7

Figure Lengend Snippet: Increasing serum IL-26 concentrations correlate with surrogate markers of asthma severity in allergen-sensitized subjects. Serum concentrations of IL-26 were measured by ELISA in allergen-sensitized subjects with asthma ( n = 51). ( A ) IL-26 concentrations were plotted relative to each subject’s daily inhaled corticosteroid dose. Statistical significance was assessed using the Spearman correlation coefficient. ( B ) IL-26 concentrations were compared between subjects who had no prior hospitalizations vs. one or more hospitalizations for asthma in the 12 months preceding study enrolment. ( C ) IL-26 concentrations were compared between subjects who had received no oral steroid vs. those receiving one or more courses of oral steroids in the 12 months preceding study enrolment. ( B - C ) Statistical significance was assessed using the Mann-Whitney test. Error bars represent mean ± SEM. Dashed horizonal line represents the lower limit of detection of the ELISA assay

Article Snippet: IL-26 was measured from serum using the Human IL-26 ELISA kit from Invitrogen (Carlsbad, CA, USA), which has an assay range between 3.3 and 800 pg/mL.

Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Critical Care

Article Title: Interleukin-26 is overexpressed in human sepsis and contributes to inflammation, organ injury, and mortality in murine sepsis

doi: 10.1186/s13054-019-2574-7

Figure Lengend Snippet: Serum IL-26 levels at admission were elevated in the patients with sepsis. a IL-26 concentrations were measured by ELISA in serum samples collected from 52 patients with sepsis, 18 non-septic ICU controls, and 30 healthy control subjects. b IL-26 concentrations in serum samples collected from septic shock patients and patients without shock. c IL-26 concentrations in serum samples collected from survivors and nonsurvivors in the patients with sepsis. d IL-26 concentrations in serum samples collected from septic patients with bacteremia and patients without bacteremia. Non-parametric Mann–Whitney U test or Kruskal-Wallis test followed by Dunn’s multiple comparisons post test was used to compare results between groups. Horizontal bars represent median values, and dots represent individual participants

Article Snippet: The concentration of human IL-26 was determined by human IL-26 ELISA kit (LifeSpan Biosciences).

Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Critical Care

Article Title: Interleukin-26 is overexpressed in human sepsis and contributes to inflammation, organ injury, and mortality in murine sepsis

doi: 10.1186/s13054-019-2574-7

Figure Lengend Snippet: Serum IL-26 levels at admission correlated with disease severity in septic patients. a Correlation of IL-26 levels with Sequential Organ Failure Assessment (SOFA) scores in the patients with sepsis. b Correlation of IL-26 levels with the length of ICU stay in the patients with sepsis. c Correlation of IL-26 levels with procalcitonin (PCT) levels in the patients with sepsis. d Correlation of IL-26 levels with C-reactive protein (CRP) levels in the patients with sepsis. Spearman’s correlation coefficient was used to evaluate the correlation between concentrations of IL-26 and SOFA scores, the length of ICU stay, PCT, or CRP levels. Horizontal bars represent median values, and dots represent individual participants

Article Snippet: The concentration of human IL-26 was determined by human IL-26 ELISA kit (LifeSpan Biosciences).

Techniques:

Journal: Critical Care

Article Title: Interleukin-26 is overexpressed in human sepsis and contributes to inflammation, organ injury, and mortality in murine sepsis

doi: 10.1186/s13054-019-2574-7

Figure Lengend Snippet: Receiver operating characteristic curve (ROC) of IL-26 for diagnosis of sepsis. Areas under the ROC curve for IL-26, 0.786 ( p = 0.01)

Article Snippet: The concentration of human IL-26 was determined by human IL-26 ELISA kit (LifeSpan Biosciences).

Techniques:

Journal: Critical Care

Article Title: Interleukin-26 is overexpressed in human sepsis and contributes to inflammation, organ injury, and mortality in murine sepsis

doi: 10.1186/s13054-019-2574-7

Figure Lengend Snippet: a Receiving operating characteristic (ROC) curve of IL-26, SOFA score, PCT, and CRP at admission for predicting 28-day mortality in septic patients. Area under the ROC curve, 0.690 ( p = 0.036) for IL-26, 0.759 ( p = 0.019) for SOFA score, 0.552 ( p = 0.091) for PCT, and 0.370 ( p = 0.265) for CRP. b Receiving operating characteristic (ROC) curve of IL-26 in combination with SOFA score for predicting 28-day mortality in septic patients. Area under ROC curve for the combination of IL-26 and SOFA score, 0.771 ( p = 0.017)

Article Snippet: The concentration of human IL-26 was determined by human IL-26 ELISA kit (LifeSpan Biosciences).

Techniques:

Journal: Critical Care

Article Title: Interleukin-26 is overexpressed in human sepsis and contributes to inflammation, organ injury, and mortality in murine sepsis

doi: 10.1186/s13054-019-2574-7

Figure Lengend Snippet: Effect of recombinant human IL-26 on cecal ligation and puncture (CLP)-induced inflammation and injury of vital organs and death. a Survival of CLP mice ( n = 20 per group) after treatment with recombinant human IL-26 in the absence or presence of CLP-induced sepsis. Recombinant human IL-26 was injected intraperitoneally at 0.5–1.0 μg/injection immediately after CLP, and PBS was delivered in a similar fashion as control solutions. Comparison between groups was done by Kaplan–Meier analysis followed by log-rank tests. Results are representative of three independent experiments. b Serological markers of organ injury including alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and creatinine in mice ( n = 5 per group) treated with or without recombinant IL-26 (0.5 μg) at 24 h after CLP. Statistical difference was denoted by the horizontal bracket (Mann–Whitney U test). c Representative examples of hematoxylin and eosin (H&E)-stained lung, liver, spleen, and kidney tissues from mice ( n = 5 per group) treated with or without recombinant human IL-26 (0.5 μg) at 24 h after CLP. d Histological scores for lung, liver, spleen, and kidney tissues from mice ( n = 5 per group) treated with or without recombinant human IL-26 (0.5 μg) at 24 h after CLP. Statistical difference was denoted by the horizontal bracket (Mann–Whitney U test)

Article Snippet: The concentration of human IL-26 was determined by human IL-26 ELISA kit (LifeSpan Biosciences).

Techniques: Recombinant, Ligation, Injection, MANN-WHITNEY, Staining

Journal: Critical Care

Article Title: Interleukin-26 is overexpressed in human sepsis and contributes to inflammation, organ injury, and mortality in murine sepsis

doi: 10.1186/s13054-019-2574-7

Figure Lengend Snippet: Administration with recombinant human IL-26 exaggerated the production of proinflammatory cytokines and chemokines in CLP-induced sepsis. Cytokine and chemokine concentrations in peritoneal lavage fluid (PLF) and blood from septic mice ( n = 5) treated with or without recombinant human IL-26 (0.5 μg) were determined by ELISA at 24 h after CLP. Statistical difference was denoted by the horizontal bracket (Mann–Whitney U test)

Article Snippet: The concentration of human IL-26 was determined by human IL-26 ELISA kit (LifeSpan Biosciences).

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Critical Care

Article Title: Interleukin-26 is overexpressed in human sepsis and contributes to inflammation, organ injury, and mortality in murine sepsis

doi: 10.1186/s13054-019-2574-7

Figure Lengend Snippet: Administration with recombinant human IL-26 increased bacterial clearance in CLP-induced sepsis. Dilutions of peritoneal lavage fluid (PLF), spleen homogenates, and blood obtained from CLP mice ( n = 5) treated with or without recombinant human IL-26 (0.5 μg) were cultured on blood agar plates, and the number of bacterial colonies was counted as colony-forming unit (CFU). Statistical difference was denoted by the horizontal bracket (Mann–Whitney U test)

Article Snippet: The concentration of human IL-26 was determined by human IL-26 ELISA kit (LifeSpan Biosciences).

Techniques: Recombinant, Cell Culture, MANN-WHITNEY

Journal: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research

Article Title: Adipose-Derived Mesenchymal Stem Cells from Arthritis Patients: Differential Modulation of CD4 + T Cell Activation and Cytokine Production

doi: 10.12659/MSM.945273

Figure Lengend Snippet: Details of the kits used for the detection of soluble factors in co-culture supernatants.

Article Snippet: IL-26 , Human IL-26 ELISA Kit , FineTest , 15.625–1000 pg/ml.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Increased interleukin-26 in the peripheral joints of patients with axial spondyloarthritis and psoriatic arthritis, co-localizing with CD68-positive synoviocytes

doi: 10.3389/fimmu.2024.1355824

Figure Lengend Snippet: Increased IL-26 levels in the serum of psoriatic arthritis (PsA) and rheumatoid arthritis (RA) patients. IL-26 serum levels were quantified by ELISA in 35 HCs (white), 29 axial spondyloarthritis (axSpA) (black), 20 PsA (red) and 15 RA (blue) patients. Preincubation with heat-aggregated bovine IgG and signal amplification with biotinyl-tyramide were applied in order to minimize false results through interfering antibodies . Statistical analysis: mean ± SEM, ** p < 0.01, *** p < 0.001 (one-way ANOVA followed by Dunnett post-test for multiple comparisons).

Article Snippet: Serum levels of IL26 were analyzed with a modified IL26 ELISA kit (Elabscience, Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Amplification

Journal: Frontiers in Immunology

Article Title: Increased interleukin-26 in the peripheral joints of patients with axial spondyloarthritis and psoriatic arthritis, co-localizing with CD68-positive synoviocytes

doi: 10.3389/fimmu.2024.1355824

Figure Lengend Snippet: IL26 -expressing cells are only slightly increased in circulating CD4 + and CD8 + T cells from patients with axSpA and PsA. (A) Representative flow cytometry plots of total IL26 + CD4 + T cells with the PrimeFlow assay in a PsA patient. RPLA13A represents the positive control for the assay. (B) Percentages of total IL26 + CD4 + T cells (left) and percentages of IL26 + cells of CD26 + CD161 + CD4 + T cells (right) for HCs ( n = 15; white) and axSpA ( n = 15; black) and PsA ( n = 14; red) patients gated from PBMCs stimulated in vitro with PMA and ionomycin in the PrimeFlow assay. (C) Representative flow cytometry plots of total IL-1R1 + Th17 cells in a PsA patient comparing IL-2 stimulation to unstimulated conditions. (D) Percentages of IL-1R1 + Th17 cells (Th17 cells gated as CD4 + CD45RO + CD161 + CCR6 + CCR4 + CXCR3 − ) of CD4 + cells from HCs ( n = 21; white) and axSpA ( n = 22; black), PsA ( n = 14; red), and RA ( n = 13; blue) patients after in vitro stimulation with IL-2 (1,000 IU/ml) overnight (left) and percentages of IL-1R1 + CD26 + CD161 + cells of CD4 + cells (right). (E) Percentages of total IL26 + CD8 + T cells (left) and percentages of IL26 + cells of CD26 + CD161 + CD8 + T cells (right) for HCs ( n = 15; white) and axSpA ( n = 15; black) and PsA ( n = 14; red) patients gated from PBMCs stimulated in vitro with PMA and ionomycin. Statistical analysis: mean ± SEM, * p < 0.05, ** p < 0.01 (one-way ANOVA followed by Dunnett post-test for multiple comparisons).

Article Snippet: Serum levels of IL26 were analyzed with a modified IL26 ELISA kit (Elabscience, Wuhan, China).

Techniques: Expressing, Flow Cytometry, Positive Control, In Vitro

Journal: Frontiers in Immunology

Article Title: Increased interleukin-26 in the peripheral joints of patients with axial spondyloarthritis and psoriatic arthritis, co-localizing with CD68-positive synoviocytes

doi: 10.3389/fimmu.2024.1355824

Figure Lengend Snippet: CD4 + T cells from axSpA patients are prone to IL26 expression under typical Th17-favoring conditions in vitro . (A) Total CD4 + T cells from HCs ( n = 20; white) and axSpA ( n = 19; black), PsA ( n = 18; red), and RA n = 13; blue) patients were incubated with different Th17-favoring cytokine combinations in vitro for 6 days, and the expression of IL26 and IL17A was analyzed by qPCR in triplicates. Expression was normalized to two control genes ( ACT and RPL13A ) and is shown as fold expression in relation to basal stimulation with IL-2 only. (B) CD4 + T cells from the same cohort as in (A) were stimulated with IL-2, IL-1β, and IL-23 for 6 days in vitro , and fold expression (triplicates each) of the respective genes is shown in relation to HCs after normalizations for ACT and RLP13A . Statistical analysis: mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001 [ (A) repeated-measures ANOVA and (B) one-way ANOVA followed by Dunnett post-test for multiple comparisons].

Article Snippet: Serum levels of IL26 were analyzed with a modified IL26 ELISA kit (Elabscience, Wuhan, China).

Techniques: Expressing, In Vitro, Incubation, Control