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mir 190a 5p  (MedChemExpress)
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MedChemExpress mir 190a 5p
Effect of transfection of human colonic epithelial NCM460 <t>cells</t> <t>with</t> <t>miR-190a-5p</t> mimic on the level of mRNA expression of: ( A ) ZO1 and ( B ) LGR5. Human colonic epithelial NCM460 cells were transfected with either a miRNA mimic negative control (200 nM) or miR-190a-5p mimic (200 nM) or miR-190a-5p mimic (200 nM) for 48 h, and then ZO1 and LGR5 mRNA expression levels were quantified by RT-qPCR as described in . Data from RT-qPCR were normalized to the internal control β-actin. Data are presented as means ± SE from 3–4 independent experiments (* p < 0.01).
Mir 190a 5p, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of transfection of human colonic epithelial NCM460 cells with miR-190a-5p mimic on the level of mRNA expression of: ( A ) ZO1 and ( B ) LGR5. Human colonic epithelial NCM460 cells were transfected with either a miRNA mimic negative control (200 nM) or miR-190a-5p mimic (200 nM) or miR-190a-5p mimic (200 nM) for 48 h, and then ZO1 and LGR5 mRNA expression levels were quantified by RT-qPCR as described in . Data from RT-qPCR were normalized to the internal control β-actin. Data are presented as means ± SE from 3–4 independent experiments (* p < 0.01).

Journal: Nutrients

Article Title: Biotin Deficiency Alters the Expression Profile of Colonic microRNAs: Possible Contribution to the Alterations in Expression of Proteins Involved in the Maintenance of Colonic Physiology and Inflammation

doi: 10.3390/nu18040612

Figure Lengend Snippet: Effect of transfection of human colonic epithelial NCM460 cells with miR-190a-5p mimic on the level of mRNA expression of: ( A ) ZO1 and ( B ) LGR5. Human colonic epithelial NCM460 cells were transfected with either a miRNA mimic negative control (200 nM) or miR-190a-5p mimic (200 nM) or miR-190a-5p mimic (200 nM) for 48 h, and then ZO1 and LGR5 mRNA expression levels were quantified by RT-qPCR as described in . Data from RT-qPCR were normalized to the internal control β-actin. Data are presented as means ± SE from 3–4 independent experiments (* p < 0.01).

Article Snippet: Transient transfection of NCM460 cells was done using 200 nM miRNA mimic miR-190a-5p (MCE MedChemExpress, Cat# HY- R00361 ) and miR-199a-5p (MCE MedChemExpress, Cat# HY- R00399 ) with appropriate negative control miRNA mimic (MCE MedChemExpress, Cat# HY- R04602 ) for 48 h, using RNAiMax (Cat# 13778150) transfection reagent to achieve maximum transfection efficiency (Invitrogen, Carlsbad, CA, USA).

Techniques: Transfection, Expressing, Negative Control, Quantitative RT-PCR, Control