Journal: International Journal of Molecular Sciences
Article Title: Cinobufagin Exerts Anticancer Activity in Oral Squamous Cell Carcinoma Cells through Downregulation of ANO1
Figure Lengend Snippet: Effect of cinobufagin on cell viability and migration in CAL-27 cells. ( A ) Effect of cinobufagin on cell viability in CAL-27, and ANO1 knockout (KO) CAL-27 cells. These cells were treated with cinobufagin at the indicated concentrations for 48 h, and MTS assay was performed to estimate cell viability (mean ± S.E., n = 5). ( B , C ) Scratch wound healing assay was conducted in CAL-27 cells expressing high levels of ANO1. Cells were treated with cinobufagin at the indicated concentration, and time-lapse images were acquired every 2 h after wound generation (mean ± S.E., n = 3). Scale bars represent 300 μm. * p
Article Snippet: Cinobufagin was purchased from MedChem Express (Monmouth Junction, NJ, USA).
Techniques: Migration, Knock-Out, MTS Assay, Wound Healing Assay, Expressing, Concentration Assay