HY-18749 Search Results


p akt agonist sc79  (MedChemExpress)
99
MedChemExpress p akt agonist sc79
Lactate modulated hepatic IL-33 and neutrophilic ST2 expression via AKT/Fos and JAK/STAT3 pathways. A KEGG pathway analysis of DEGs in hepatocytes was performed. B Heatmap showing expression profiles of PI3K-AKT pathway-related genes. C Western blot analysis of p-AKT, AKT and β-ACTIN in hepatocytes exposed to H/R with or without lactate. Densitometric analysis was performed by expressing phospho-AKT as the ratio of phospho/total AKT. D Representative flow cytometry profiles (left) and quantification (right) of p-AKT MFI in hepatocytes exposed to H/R with or without lactate. E Bioinformatic prediction of c-Fos-binding sites in the IL-33 promoter region. F Relative Fos mRNA levels in hepatocytes from HIRI mice ± lactate. G Representative flow cytometry profiles (left) and quantification (right) of c-Fos MFI in hepatocytes exposed to H/R ± lactate. H Flow cytometry of c-Fos and IL-33 expression levels of hepatocytes with different conditions (H/R, lactate, <t>SC79</t> and MK2206). I KEGG pathway analyses of downregulated DEGs in neutrophils. J Bioinformatic prediction of STAT3-binding sites in the ST2 promoter region. K Relative Stat3 mRNA levels in neutrophils from HIRI mice with or without lactate. L Western blot analysis of phospho STAT3, STAT3 and β-ACTIN in neutrophils stimulated with LPS with or without lactate. Densitometric analysis was performed by expressing phospho-STAT3 as the ratio of phospho/total STAT3. M Representative flow cytometry profiles (left) and quantification (right) of p-STAT3 MFI in neutrophils stimulated with LPS with or without lactate. N Flow cytometry of ST2 expression levels of neutrophils with different conditions (LPS, lactate, ML115 and Stattic). O Proposed mechanism of lactate regulation in hepatocytes and neutrophils during HIRI. Data shown represent mean ± SD. D , G , H , M , and N Each group included five biologically independent replicates. C , F , K and L Each group included three biologically independent replicates. F , K Two-tailed unpaired Student’s t test. (C, D, G, L, H, M, and N) One-way ANOVA followed by Tukey’s correction. * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001, ns, no significance
P Akt Agonist Sc79, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/HY-18749/SC79/pmc12895952-58-27-31
Average 99 stars, based on 1 article reviews
p akt agonist sc79 - by Bioz Stars, 2026-09
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Lactate modulated hepatic IL-33 and neutrophilic ST2 expression via AKT/Fos and JAK/STAT3 pathways. A KEGG pathway analysis of DEGs in hepatocytes was performed. B Heatmap showing expression profiles of PI3K-AKT pathway-related genes. C Western blot analysis of p-AKT, AKT and β-ACTIN in hepatocytes exposed to H/R with or without lactate. Densitometric analysis was performed by expressing phospho-AKT as the ratio of phospho/total AKT. D Representative flow cytometry profiles (left) and quantification (right) of p-AKT MFI in hepatocytes exposed to H/R with or without lactate. E Bioinformatic prediction of c-Fos-binding sites in the IL-33 promoter region. F Relative Fos mRNA levels in hepatocytes from HIRI mice ± lactate. G Representative flow cytometry profiles (left) and quantification (right) of c-Fos MFI in hepatocytes exposed to H/R ± lactate. H Flow cytometry of c-Fos and IL-33 expression levels of hepatocytes with different conditions (H/R, lactate, SC79 and MK2206). I KEGG pathway analyses of downregulated DEGs in neutrophils. J Bioinformatic prediction of STAT3-binding sites in the ST2 promoter region. K Relative Stat3 mRNA levels in neutrophils from HIRI mice with or without lactate. L Western blot analysis of phospho STAT3, STAT3 and β-ACTIN in neutrophils stimulated with LPS with or without lactate. Densitometric analysis was performed by expressing phospho-STAT3 as the ratio of phospho/total STAT3. M Representative flow cytometry profiles (left) and quantification (right) of p-STAT3 MFI in neutrophils stimulated with LPS with or without lactate. N Flow cytometry of ST2 expression levels of neutrophils with different conditions (LPS, lactate, ML115 and Stattic). O Proposed mechanism of lactate regulation in hepatocytes and neutrophils during HIRI. Data shown represent mean ± SD. D , G , H , M , and N Each group included five biologically independent replicates. C , F , K and L Each group included three biologically independent replicates. F , K Two-tailed unpaired Student’s t test. (C, D, G, L, H, M, and N) One-way ANOVA followed by Tukey’s correction. * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001, ns, no significance

Journal: Cell Communication and Signaling : CCS

Article Title: Lactate attenuates hepatic ischemia-reperfusion injury by reducing intrahepatic inflammation and suppressing hepatocyte-neutrophil interaction via the IL-33/ST2 pathway

doi: 10.1186/s12964-026-02662-0

Figure Lengend Snippet: Lactate modulated hepatic IL-33 and neutrophilic ST2 expression via AKT/Fos and JAK/STAT3 pathways. A KEGG pathway analysis of DEGs in hepatocytes was performed. B Heatmap showing expression profiles of PI3K-AKT pathway-related genes. C Western blot analysis of p-AKT, AKT and β-ACTIN in hepatocytes exposed to H/R with or without lactate. Densitometric analysis was performed by expressing phospho-AKT as the ratio of phospho/total AKT. D Representative flow cytometry profiles (left) and quantification (right) of p-AKT MFI in hepatocytes exposed to H/R with or without lactate. E Bioinformatic prediction of c-Fos-binding sites in the IL-33 promoter region. F Relative Fos mRNA levels in hepatocytes from HIRI mice ± lactate. G Representative flow cytometry profiles (left) and quantification (right) of c-Fos MFI in hepatocytes exposed to H/R ± lactate. H Flow cytometry of c-Fos and IL-33 expression levels of hepatocytes with different conditions (H/R, lactate, SC79 and MK2206). I KEGG pathway analyses of downregulated DEGs in neutrophils. J Bioinformatic prediction of STAT3-binding sites in the ST2 promoter region. K Relative Stat3 mRNA levels in neutrophils from HIRI mice with or without lactate. L Western blot analysis of phospho STAT3, STAT3 and β-ACTIN in neutrophils stimulated with LPS with or without lactate. Densitometric analysis was performed by expressing phospho-STAT3 as the ratio of phospho/total STAT3. M Representative flow cytometry profiles (left) and quantification (right) of p-STAT3 MFI in neutrophils stimulated with LPS with or without lactate. N Flow cytometry of ST2 expression levels of neutrophils with different conditions (LPS, lactate, ML115 and Stattic). O Proposed mechanism of lactate regulation in hepatocytes and neutrophils during HIRI. Data shown represent mean ± SD. D , G , H , M , and N Each group included five biologically independent replicates. C , F , K and L Each group included three biologically independent replicates. F , K Two-tailed unpaired Student’s t test. (C, D, G, L, H, M, and N) One-way ANOVA followed by Tukey’s correction. * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001, ns, no significance

Article Snippet: In some experiments, primary hepatocytes (PHCs) were stimulated in the presence or absence of 20 mM sodium lactate, 2.5 μM p-AKT inhibitor MK2206 (HY-108232, MCE), 10 μM p-AKT agonist SC79 (HY-18749, MCE), 5 ng/mL TGF-β1 (HY-P7117, MCE), PDGF-BB (HY-P7087, MCE), 25 ng/mL IL-1β (HY-P7073, MCE), 20 ng/mL TNF-α (HY-P7090, MCE), 10 ng/mL IFNγ (HY-P7071, MCE), 15 mM LDHA inhibitor Oxamate (HY-W013032A, MCE) and 5 mM GPR81 antagonist 3-Hydroxybutyric acid sodium (HY-W010452, MCE).

Techniques: Expressing, Western Blot, Flow Cytometry, Binding Assay, Two Tailed Test