Journal: International journal of pharmaceutics

Article Title: Polymeric nanofiber leveraged co-delivery of anti-stromal PAK1 inhibitor and paclitaxel enhances therapeutic effects in stroma-rich 3D spheroid models.

doi: 10.1016/j.ijpharm.2024.124078

Figure Lengend Snippet: Fig. 2. Characterization of drug-loaded electrospun NFs. (A) SEM images of empty-, PTX-, FRAX- and combi (PTX + FRAX597) NFs. Scale bar of main images: 20 µm, zoom-in images: 5 µm. (B) NFs diameter distribution histograms and non-linear gaussian regression curves. Bar charts representing (C) mean diameter and (D) mean pore area of NFs. (E) Non-linear regression curves showing cumulative drug release profiles of PTX and FRAX597 from single drug-loaded PTX NF and FRAX NF, respectively and Combi NF. Graphical data represent mean ± SEM (n = 3).

Article Snippet: To prepare PTX (LC Laboratories, MA) and FRAX597 (FRAX; MedChemExpress, NJ)incorporated NFs, PTX and/or FRAX597 were added to the polymer solution at a final concentration of 144 x 10-6 M and stirred for an additional 24 h. The electrospinning solution mixtures were placed into 10-ml syringes and loaded on a programmed syringe pump.

Techniques:

Journal: International journal of pharmaceutics

Article Title: Polymeric nanofiber leveraged co-delivery of anti-stromal PAK1 inhibitor and paclitaxel enhances therapeutic effects in stroma-rich 3D spheroid models.

doi: 10.1016/j.ijpharm.2024.124078

Figure Lengend Snippet: Fig. 4. Inhibitory effects of PTX monotherapy and in combination with FRAX597 on 3D murine breast tumor spheroid model. (A, C) Growth curves and (B, D) representative microscopic images of 4T1 homospheroids and 4T1/3T3 heterospheroids treated with increasing PTX concentrations with/or without FRAX597, respectively. Arrows indicate the onset of treatment. Scale bar: 500 µm. The bar charts show the quantitation of the effect on day 12. The changes in the perimeter and shape of spheroids are drawn manually. (E) Bar chart showing the comparative effect of combined PTX + FRAX597 versus PTX alone (set at 100 %) at different concentrations in 4T1 homo- and 4T1/3T3 heterospheroids on day 12 of treatment. Graphical data represent mean ± SEM (n = 6). Statistical analysis represents two- tailed unpaired student t-test for 2-group comparisons, while One-Way ANOVA, using Tukey post-hoc test for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: To prepare PTX (LC Laboratories, MA) and FRAX597 (FRAX; MedChemExpress, NJ)incorporated NFs, PTX and/or FRAX597 were added to the polymer solution at a final concentration of 144 x 10-6 M and stirred for an additional 24 h. The electrospinning solution mixtures were placed into 10-ml syringes and loaded on a programmed syringe pump.

Techniques: Quantitation Assay, Two Tailed Test

Journal: Brazilian Journal of Medical and Biological Research

Article Title: FRAX486, a PAK inhibitor, overcomes ABCB1-mediated multidrug resistance in breast cancer cells

doi: 10.1590/1414-431X2024e13357

Figure Lengend Snippet: Cytotoxicity of FRAX486 in parental and ABCB1-overexpressing cells.

Article Snippet: We purchased FRAX486, paclitaxel, doxorubicin, cisplatin, and verapamil from MedChemExpress (USA).

Techniques:

Journal: Brazilian Journal of Medical and Biological Research

Article Title: FRAX486, a PAK inhibitor, overcomes ABCB1-mediated multidrug resistance in breast cancer cells

doi: 10.1590/1414-431X2024e13357

Figure Lengend Snippet: Effect of FRAX486 on intracellular accumulation and efflux of doxorubicin in drug-sensitive and drug-resistant cells. A , Accumulation. B , Efflux. Data are reported as means±SD. *P<0.05 (ANOVA).

Article Snippet: We purchased FRAX486, paclitaxel, doxorubicin, cisplatin, and verapamil from MedChemExpress (USA).

Techniques: